nanoString nCounter Pro, Руководство пользователя

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SEV-00184-01 nCounter Pro Analysis System User Manual
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
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4. The ‘Select sample tubes’ screen will
appear (Figure 15). Select the sample
positions that will be processed. Green
tubes will be processed, and grey tubes will
not be processed. If processing fewer than
12 tubes, begin with tube 1. Press next.
NOTE: Each prep plate contains enough
reagents for 6 cartridge lanes. If processing
more than 6 lanes, use two prep plates. If
processing 6 lanes or less, use only one prep
plate, placed in the front position, and
ensure the hybridized samples are in tubes
1-6 of the 12-tube strip.
Any number of lanes between 1–12 will still consume an entire Cartridge.
5. The ‘Warm Reagents & Cartridge’ screen
will appear (Figure 16). Cartridges and
reagent plates (prep plates) (Figure 17)
must be at room temperature prior to
processing. Remove the prep plates from
storage at 4°C and the nCounter
Cartridges from storage at -20°C. Allow
them to equilibrate to room temperature
for 10–15 minutes.
NOTE: If the cartridges and prep plates are
not at room temperature prior to use, assay
variability may increase. Do not open the
cartridge pouch until it has reached room
temperature. This will prevent
condensation on the cartridge.
Figure 15: The ‘Select sample tubes’ screen.
Figure 16: The ‘Warm Reagents & Cartridge’
screen.
Figure 17: A sealed cartridge (left) and reagent plate (‘prep plate’) (right).