NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
76
11. Apply sample to the sample window and press the sample button to
initiate the measurement.
C
ALCULATIONS
Absorbance Calculation:
Absorbance is formally defined as the decimal logarithm (base 10) of the reciprocal of
transmittance:
A
= log
�
1
T
�
=
- log T
T
=
10
(-A)
Note
: Corresponding absorbance value e.g. Absorbance value (
λ
= 230) etc. normalized to
10 mm path length
%Transmittance Calculations (cuvette mode only)
In the wavelength application it is possible to measure the absorbance (A) and % transmittance
(%T) of a sample with respect to a reference at a specific wavelength. Transmittance is the ratio
of light intensity remaining after it has passed through the sample (
I
) to the initial incident light
intensity (
I
0
):
𝑇𝑇 =
𝐼𝐼
𝐼𝐼
0
%𝑇𝑇 =
𝐼𝐼
𝐼𝐼
0
× 100
M
ORE
A
PPS
:
W
AVESCAN
M
ETHOD
O
VERVIEW
Using the wavescan application it is possible to obtain the complete spectral scan for a defined
wavelength range between 200-900 nm (NP80/N60/C40) or from 200-650 nm (N50).
M
EASUREMENT
P
ROTOCOL
1. Select the More Apps icon from the Home screen and the Wavescan
icon from the More Apps screen
2.
To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
