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NanoPhotometer

®

 

NP80/N60/N50/C40

 

User Guide Version 3.1

 

 

3  

 

Contents

 

1.

 

N

ANO

P

HOTOMETER

®

 AT A 

G

LANCE

 ....................................................................... 8

 

N

ANO

P

HOTOMETER

®

 

O

VERVIEW

 .............................................................................................. 8

 

N

ANO

P

HOTOMETER

®

 

NP80 ................................................................................................................ 9

 

N

ANO

P

HOTOMETER

®

 

N60 ................................................................................................................. 10

 

N

ANO

P

HOTOMETER

®

 

N50 ................................................................................................................. 11

 

N

ANO

P

HOTOMETER

®

 

C40 ................................................................................................................. 12

 

I

NSTRUMENT 

R

EAR 

P

ANEL 

(NP80/N60/N50/C40) .............................................................................. 13

 

I

NSTRUMENT 

B

OTTOM 

V

IEW 

(NP80/N60/N50/C40) ............................................................................ 13

 

A

CCESSORIES

 ....................................................................................................................... 14

 

S

TANDARD 

A

CCESSORIES

 ................................................................................................................. 14

 

O

PTIONAL 

A

CCESSORIES

 .................................................................................................................. 14

 

C

ONNECTIVITY

....................................................................................................................... 18

 

N

ANO

P

HOTOMETER

®

 

S

PECIFICATIONS

 .................................................................................... 19

 

2.

 

G

ETTING 

S

TARTED

 .............................................................................................. 21

 

S

PECTROPHOTOMETER 

I

NSTALLATION

 .................................................................................... 21

 

S

AFETY 

I

NFORMATION

 ...................................................................................................................... 21

 

U

NPACKING AND 

P

OSITIONING

 .......................................................................................................... 22

 

S

OFTWARE 

I

NSTALLATION

 ...................................................................................................... 23

 

NPOS

 

O

VERVIEW

 ............................................................................................................................. 23

 

R

EQUIREMENTS AND 

C

OMPATIBILITY

 ................................................................................................. 23

 

I

NSTALLING 

S

OFTWARE ON COMPUTER

 .............................................................................................. 24

 

I

NSTALLING 

N

ANO

P

HOTOMETER

®

 

A

PP ON 

T

ABLET OR 

S

MARTPHONE

 .................................................. 25

 

F

IRST STEPS AND CONFIGURATION WIZARD

 ........................................................................................ 26

 

P

RINTER 

I

NSTALLATION

 .................................................................................................................... 26

 

3.

 

N

ANO

P

HOTOMETER

®

 

B

ASICS

 .............................................................................. 27

 

A

PPLICATIONS 

O

VERVIEW

 ...................................................................................................... 27

 

I

CONS

 ................................................................................................................................... 29

 

B

UTTONS

 .............................................................................................................................. 30

 

S

IDE 

T

AB BAR

 .................................................................................................................................. 31

 

D

ATA PROCESSING DIALOGS

 ............................................................................................................. 33

 

B

ASIC 

O

PERATION

 ................................................................................................................. 36

 

N

ANO

V

OLUME 

M

EASUREMENT 

B

ASICS 

(N50/N60/NP80) ................................................................... 36

 

C

UVETTE 

M

EASUREMENT 

B

ASICS 

(C40/NP80) .................................................................................. 37

 

S

UBMICROLITER 

C

ELL 

M

EASUREMENTS 

B

ASICS 

(C40)....................................................................... 39

 

S

AMPLE 

H

ANDLING 

T

IPS

 ................................................................................................................... 40

 

D

ATA 

T

RANSFER

 .............................................................................................................................. 42

 

Содержание N50

Страница 1: ...NanoPhotometer NP80 N60 N50 C40 User Manual Version 3 1 Software Version 3 1 13220...

Страница 2: ...47 CFR Part15 15 107 and 15 109 EN 301 489 1 V1 9 2 Radio and ancillary equipment for portable use portable equipment EUT Operating frequency range 2 4 2 4835 GHz EN 300 328 V1 8 1 Electromagnetic co...

Страница 3: ...21 SPECTROPHOTOMETER INSTALLATION 21 SAFETY INFORMATION 21 UNPACKING AND POSITIONING 22 SOFTWARE INSTALLATION 23 NPOS OVERVIEW 23 REQUIREMENTS AND COMPATIBILITY 23 INSTALLING SOFTWARE ON COMPUTER 24 I...

Страница 4: ...COL 62 CALCULATIONS 66 KINETICS 66 METHOD OVERVIEW 66 MEASUREMENT PROTOCOL 66 CALCULATIONS 68 OD600 68 METHOD OVERVIEW 68 MEASUREMENT PROTOCOL 70 CALCULATIONS 72 MORE APPS 73 MORE APPS WAVELENGTH 73 M...

Страница 5: ...92 ABOUT 93 LANGUAGE 93 ILLUMINATION SAMPLE WINDOW 94 ENABLE NANOVOLUME 94 DYES 94 WARNING MESSAGES 95 BLANK CONTROL 95 SAMPLE QUALITY CONTROL 95 NETWORK 96 WIFI 97 NETWORK PRINTER 97 REPORT CONFIGUR...

Страница 6: ...AATSEN 113 12 APPENDIX 3 INSTALLATION DU SPECTROPHOTOM TRE 114 INFORMATIONS DE S CURIT 114 D BALLAGE ET POSITIONNEMENT 115 13 APPENDIX 4 INSTALLATION SPECTROPHOTOMETER 117 SICHERHEITSINFORMATIONEN 117...

Страница 7: ...0 C40 User Guide Version 3 1 7 S KERHETSINFORMATION 126 UPPACKNING OCH PLACERING 127 18 APPENDIX 9 INSTALA O DE ESPECTROFOT METRO 128 INFORMA ES DE SEGURAN A 128 DESEMBALAMENTO E POSICIONAMENTO 129 19...

Страница 8: ...nmatched precision and accuracy without the need for dilutions Combined with our True Path Technology the system offers lifetime accuracy and precision without the need for maintenance or recalibratio...

Страница 9: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 9 NANOPHOTOMETER NP80 Item Number Touchscreen Battery Pack NP80 NP80 Touch NP80 Mobile...

Страница 10: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 10 NANOPHOTOMETER N60 Item Number Touchscreen Battery Pack N60 N60 Touch N60 Mobile...

Страница 11: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 11 NANOPHOTOMETER N50 Item Number Touchscreen Battery Pack N50 N50 Touch...

Страница 12: ...NanoPhotometer NP80 N60 N50 C40 User Guide Version 3 1 12 NANOPHOTOMETER C40 Item Number Touchscreen Battery Pack C40 C40 Touch C40 Mobile...

Страница 13: ...3 seconds initiate a hard reset Only activate a hard reset of the unit when necessary To avoid excess hard reboots it is recommended to power down the unit from the onboard touch screen by clicking o...

Страница 14: ...ation NanoPhotometer Power Adapter Power adapter for the NanoPhotometer and for charging the optional built in battery pack Note Use only the power adapter supplied with your instrument or a replaceme...

Страница 15: ...noPhotometer N50 Submicroliter Cell C40 only The submicroliter cell provides the option of upgrading the NanoPhotometer C40 to a device that handles nanovolume samples as well as cuvette samples The s...

Страница 16: ...The imported name can then be edited or replaced completely Barcode readers that have been tested and verified compatible 1D Honeywell Voyager 95X0 Single Line Laser Scanner Datalogic Touch65 1D 2D A...

Страница 17: ...3 1 17 Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450 Note The printouts are optimized for the label size of the DYMO LabelWriter 4XL All other DYMO printers can be used However t...

Страница 18: ...We recommend using FAT FAT32 formatted 2 0 USB flash drives Encrypted USB flash drives are not compatible with the NanoPhotometer Note Cordless Bluetooth mice are not supported Use wired mice only No...

Страница 19: ...s limited to 40 measurements per dataset Larger datasets can be saved on the NanoPhotometer itself HDMI There is an HDMI port located on the rear panel of the NanoPhotometer which is compatible with H...

Страница 20: ...artphone and tablet Software Compatibility Windows 7 32 and 64 bit Windows 8 32 and 64 bit OS X iOS and Android Min Requirement Smartphone Tablet 4 inch screen Apple iPad 2 iPhone 5 and iOS 6 Android...

Страница 21: ...sure when measuring without lid Do not use damaged power cords accessories and other peripherals with your NanoPhotometer Use only the delivered and specified power supply charger Do not expose the Na...

Страница 22: ...ge 10 40 C If the cuvette heating is used the range is 10 30 C If the instrument is subjected to extreme temperature changes it may be necessary to allow the instrument to equilibrate Turn the instrum...

Страница 23: ...r until the NanoPhotometer NPOS software is installed on the computer REQUIREMENTS AND COMPATIBILITY The NPOS user interface is designed that all features can be operated by using a touchscreen If the...

Страница 24: ...the update on your computer 2 Launch the NPOS installation file NanoPhotometer msi and follow the installation routine The installer should be logged in with full administration rights If you have ins...

Страница 25: ...tch off the browser safety This may interfere with the NPOS running on your computer INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE The NanoPhotometer App can be installed as an application on...

Страница 26: ...tometer with previous software versions printer driver installation is necessary 1 Switch NanoPhotometer on home screen 2 Update to newest firmware version Update Process a Copy NPOS bin to a USB flas...

Страница 27: ...an be performed with 10 mm 5 mm 2 mm 1 mm and 0 5 mm path length quartz glass or plastic cuvettes with a center height of 8 5 mm APPLICATIONS OVERVIEW The NanoPhotometer comes with pre programmed appl...

Страница 28: ...secondary method screen Wavelength Define one or multiple wavelength between 200 900 nm N50 200 650 nm for absorbance measurements Wavescan Define desired full scan range anywhere between 200 900 nm...

Страница 29: ...Delete Deletes added functions in parameter empties input windows Delete Data Opens a delete dialog pop up Email Data Opens an email dialog pop up computer and tablet versions only Save Data Opens a...

Страница 30: ...applications Selecting the Change to Cuvette button activates the cuvette compartment opens the sliding cover and switches the red arrow LED on Only cuvette measurements are possible when this mode i...

Страница 31: ...parameter area it is possible to define all necessary parameters for a measurement as well as turn on cuvette mode and initiate cuvette heating The default measurement screen includes the parameter ar...

Страница 32: ...asurements will be automatically overlaid To change the overlaid graphs use the tick boxes in the table Note It is only possible to overlay up to 30 graphs in a chart If more than 30 data are selected...

Страница 33: ...rk please disconnect the installed USB printer Note The print icon is only shown if a printer is available Note It is only possible to operate one printer at a time Do not connect more than one printe...

Страница 34: ...lways all measurements saved no selection possible Save as Type With the save type option it is possible to specify the file type for saving File type options include Excel PDF and Implen Document Sou...

Страница 35: ...he file Allowed characters are A Z a z 0 9 _ Note Blank character is not allowed Storage Selection option to choose where you would like the data to be saved The options include NanoPhotometer USB fla...

Страница 36: ...0 l Standard Cuvette applications can be performed with 10 mm 5 mm 2 mm 1 mm and 0 5 mm path length quartz glass or plastic cuvettes with a center height of 8 5 cm Note NanoVolume application in the...

Страница 37: ...e to enter a sample name for each sample in the input window enter sample name Note Allowed characters are A Z a z 0 9 _ Note Blank character is not allowed 8 Raise the sample arm and pipette the appr...

Страница 38: ...elow the parameter area NP80 only 3 Add a blank solution to a cuvette and ensure that the filling volume is sufficient to allow light path to pass through the solution 4 Insert cuvette into the cell h...

Страница 39: ...tion We guarantee our submicroliter cell specifications only for Implen submicroliter cells which are aligned to the NanoPhotometer C40 by Implen 3 Set the parameter for the measurement Choose the dil...

Страница 40: ...on of measurement remove the lid clean the surfaces and apply the next sample SAMPLE HANDLING TIPS Nanovolume Methods The NanoPhotometer includes an integrated vortexer N60 NP80 only to assure sample...

Страница 41: ...on the cuvette type used it is necessary that the light passes through the sample for accurate measurements Center height is 8 5 mm Note The cuvette holder is not removable Do not pour any cleaning so...

Страница 42: ...ord secured WiFi connection To access transfer data from the NanoPhotometer network drive via LAN connection the serial number or the NanoPhotometer IP needs to be entered in the address bar of the Wi...

Страница 43: ...arge the battery at this status If the battery is not charged there will be a second warning message when the battery is empty and several beep sounds before the instrument is automatically shutting d...

Страница 44: ...0 nm ratios Further it is possible to determine the degree of labeling of nucleic acids with probes including fluorescent dyes Sample Control gives useful information about sample conditions It recogn...

Страница 45: ...mm path length for samples with concentrations 420 ng l dsDNA For cuvette application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm For NanoVolume a...

Страница 46: ...ons are 320 nm 340 nm It is also possible to add any wavelength in the range of 220 350 nm 7 8 Air bubble recognition is disabled as a default Set to on it detects air bubbles lint residues and bad sa...

Страница 47: ...surement and select blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences 12 Use a lint free laboratory wipe to clean both the sample window a...

Страница 48: ...round wavelength 10 mm path Manual dilution factor nuc Extinction coefficient nucleic acid factor in ng cm l Table 1 Nucleic acids extinction coefficients nuc Type nuc dsDNA 50 ng cm l ssDNA 37 ng cm...

Страница 49: ...cid factor in ng cm l cfdye Dye dependent correction factor at 260 nm Manual dilution factor Note Dye correction is only available for single dye selection Dye Concentration For dye labeled nucleic ac...

Страница 50: ...0 ABKG cfdye Amax dye ABKG With background correction without dye correction FOI 324 5 Amax dye ABKG dye 106 A260 ABKG nuc Without background correction and with dye correction FOI 324 5 Amax dye dye...

Страница 51: ...000 0 05 NP80 N60 C40 Cy5 5 675 250 000 0 05 NP80 N60 N50 C40 Oyster 500 503 78 000 0 29 NP80 N60 N50 C40 Oyster 550 553 150 000 0 05 NP80 N60 N50 C40 Oyster 556 560 155 000 0 03 NP80 N60 C40 Oyster...

Страница 52: ...shown in the results table area A click on the alert icon shows additional information The ranges for acceptable ratio values can be defined in preferences The ratios are calculated with or without ba...

Страница 53: ...er determines protein concentration by performing calculations based on specific values either pre programmed in the instrument or entered manually by the user Extinction coefficient values at 280 nm...

Страница 54: ...the right side Options are BSA SA Mouse SA Human IgG Mouse IgG Human IgE Human Lysozyme OD1 Molar Extinction Coefficient and Extinction Coefficient For Mol Ext Coefficient enter Molecular Weight defa...

Страница 55: ...en changing the dilution lid 5 The background correction is enabled at 320 nm as a default It is possible to disable the background correction with the toggle switch Selection options are 320 nm 340 n...

Страница 56: ...dye is not available in the list please go to preferences and add a custom dye to the dye list There is an option for dye correction which can be enabled disabled with a toggle switch Note Dye correc...

Страница 57: ...button to initiate the measurement CALCULATIONS Protein UV280 Concentration The protein concentration in the Protein UV method is calculated with the absorbance value of the sample at 280 nm along wi...

Страница 58: ...tion factor at 280 nm see Table 4 The dye concentration is calculated with or without background dye correction for are as follows With background and with dye correction C A280 ABKG cfdye Amax dye AB...

Страница 59: ...ath dye Extinction coefficient of dye in M 1 cm 1 Dilution factor Degree of Labeling DOL DOL is the degree of labeling based on the average number of dye molecules coupled to a protein molecule The de...

Страница 60: ...40 Alexa Fluor 546 554 104 000 0 12 NP80 N60 N50 C40 Alexa Fluor 555 555 150 000 0 08 NP80 N60 N50 C40 Alexa Fluor 568 577 91 300 0 46 NP80 N60 N50 C40 Alexa Fluor 594 590 73 000 0 56 NP80 N60 C40 Ale...

Страница 61: ...nge at the appropriate wavelength for each assay and compared against a standard curve prepared by serial dilution of a protein standard of known concentration A linear linear zero or 2nd order regres...

Страница 62: ...h a standard protein at 750 nm this is usually BSA bovine serum albumin Note Detailed protocols are customarily supplied with these assay kits and must be closely followed to ensure that accurate resu...

Страница 63: ...s of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 5 If it is desired to...

Страница 64: ...ons are linear regression zero regression forces the straight line through the origin and 2nd order regression 8 Select Unit 9 Add up to 20 Concentrations by clicking on the Add Concentration button A...

Страница 65: ...le to exclude single measurements from the curve calculation by switching the toggle switch off Note After the first sample measurement the standard curve can no longer be altered 12 Once a Standard c...

Страница 66: ...nding changes in the concentration of reactants or products as the reaction progresses The rate of many chemical reactions can be markedly accelerated by the presence of catalysts which remain chemica...

Страница 67: ...ts Basics Required sample volume for each lid is listed to the right Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch...

Страница 68: ...nge of parameters is only possible before starting the kinetic readings Save and delete data is only available after the kinetic session is stopped Note Auto print and cryo label print is not availabl...

Страница 69: ...sample Therefore if results from different spectrophotometers are to be compared they must be normalized first using appropriate calibration curves A calibration curve can be constructed by comparing...

Страница 70: ...o Cuvette NanoVolume button below the parameter area NP80 C40 only 3 For Cuvette Application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm For NanoVo...

Страница 71: ...e the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Default wavelen...

Страница 72: ...samples 10 Insert cuvette with the reference sample and select blank button to initiate the measurement 11 Insert cuvette with the sample and select the sample button to initiate the measurement CALC...

Страница 73: ...d transmittance Trans only in cuvette mode of a sample at specific wavelengths It is possible to add up to 20 different wavelengths MEASUREMENT PROTOCOL 1 Select the More Apps icon from the home scree...

Страница 74: ...tch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 5 Enter desired wavelength to be...

Страница 75: ...les 9 Apply the blank ddH20 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading Note The illumination of the sample window can be switched...

Страница 76: ...asure the absorbance A and transmittance T of a sample with respect to a reference at a specific wavelength Transmittance is the ratio of light intensity remaining after it has passed through the samp...

Страница 77: ...5 mm and 10 mm For NanoVolume application with submicroliter cell C40 only Select Lid depending on your sample concentration for concentration ranges of different lids see page 39 Submicroliter Cell...

Страница 78: ...nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 7 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 defau...

Страница 79: ...it is possible to change to the Transmittance mode If a peak of interest is not shown in the results the peak can be selected by clicking on the graph The peak can then be added to the results by tap...

Страница 80: ...r sample concentration for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when c...

Страница 81: ...ions Off 1 boxcar 11 default 2 boxcar 21 and 3 boxcar 61 8 Option to set calculate a dilution factor for manual diluted samples 9 Apply the blank ddH20 or buffer to the illuminated sample window for t...

Страница 82: ...for a sample by determining the absorbance at a specific wavelength relative to a reference The concentration is then obtained by multiplying the measured absorbance by a specific factor This factor m...

Страница 83: ...e concentration for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing...

Страница 84: ...lt Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50...

Страница 85: ...t blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences 12 Use a lint free laboratory wipe to clean both the sample window and mirror prior to...

Страница 86: ...s of the same type with unknown concentrations This application provides an extremely useful tool with which to integrate expedite and simplify the measurement and calculations involved in determining...

Страница 87: ...r each lid is shown Note A new Blank is recommended when changing the dilution lid 4 If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette ho...

Страница 88: ...tometer NP80 N60 N50 C40 User Guide Version 3 1 88 6 Select the curve fit type Options are linear regression zero regression forces the straight line through the origin and 2nd order regression 7 Sele...

Страница 89: ...ll be used for concentration calculations in the method It might be necessary to do a blank measurement 11 Apply sample and press the sample button to initiate the measurement Note Once the sample mea...

Страница 90: ...the left side the subfolders of that storage folder and on the right side the individual files On the right side of the screen all saved result files of the selected folder are shown and can be opene...

Страница 91: ...lder shows on the left side the subfolders of the storage folder and on the right side the method files On the right side of the screen all saved method files of the selected folder are shown and can...

Страница 92: ...eral in the Preferences menu opens a window to the right of the preferences menu with the following options Display built in display only About WiFi Access Point and Illumination Sample Window NP80 N6...

Страница 93: ...following languages English Chinese French German Japanese Portuguese Russian or Spanish The language is changed for the user interface printouts and saved Excel and PDF files Note The change will aff...

Страница 94: ...s To toggle between the nucleic acid and protein list click on the Nucleic Acid Protein buttons in the header Each dye has either a lock icon in front of the dye name indicating that the dye is locked...

Страница 95: ...1 and dye dependent correction factor There is a toggle switch available to lock the dye to prevent deleting a dye from the dye list accidentally WARNING MESSAGES BLANK CONTROL Toggle switch to switc...

Страница 96: ...the DHCP protocol is selected Connect the NanoPhotometer with the Ethernet and the IP is automatically set during the startup of the NanoPhotometer If no IP Address is listed for Current IPv4 click o...

Страница 97: ...d or iPhone to the NanoPhotometer To set up a WiFi network select Connect to WiFi Network in the dropdown Select an available WiFi network from the dropdown list or choose other for hidden networks an...

Страница 98: ...e highest priority and will be the printer used by default when selecting Print on NanoPhotometer In order to print utilizing a printer on the network please disconnect the installed USB printer REPOR...

Страница 99: ...If the instrument does not pass the test a message window will appear explaining the reason for the failed test along with the recommended solution If the ok button is selected the window is closed an...

Страница 100: ...ble on computer and tablet versions only When report a problem is highlighted in the left side assistance menu the right side will have a form to fill out with information including first name last na...

Страница 101: ...before updating Note Make sure that the NanoPhotometer is connected to power and that the power connection is not interrupted during the update It is recommended to do the update via the built in touc...

Страница 102: ...he Install Driver for Printers button 5 Wait until the following message is shown Installed driver for printer successfully 6 Go to home screen 7 Connect DYMO HP printer via USB cable 8 Wait for 30 se...

Страница 103: ...contact the Implen team directly Europe Asia South Pacific Middle East and Africa Implen GmbH Schatzbogen 52 81829 M nchen Germany Phone 49 89 72637180 Fax 49 89 726371854 Email support implen de www...

Страница 104: ...on certification using filters traceable to international standards during production and quality control certified engineers and calibrated test equipment approved to ISO 9001 standard automatic self...

Страница 105: ...r bubble lint residue or a bad sample like e g a turbid sample Check sample clean the pedestal and mirror in the lid thoroughly and reapply sample carefully To avoid air bubbles apply sample by revers...

Страница 106: ...er is always empty Files are saved in the Download Folder of the Android tablet smartphone directory Instrument connected to control device Do you really want to intercept This message is shown on the...

Страница 107: ...s a higher software version than the installed one NPOS software is not downward compatible Initialization failed problem with optical path Please contact Implen customer support Check if the cuvette...

Страница 108: ...d Please contact Implen customer support Make sure that the sample name is NPOS bin without any additions or extensions like from multiple downloads otherwise rename file to be NPOS bin exactly NPOS b...

Страница 109: ...ted to ensure that it meets its published specification The warranty included in the conditions of supply is valid for 12 months and is valid only if the product has been used according to the instruc...

Страница 110: ...typ m kapalin jako je nap klad siln d nebo vlhkost Nevhazujte p stroj do ohn proto e se m e zdeformovat nebo explodovat baterie Neskladujte ani nepou vejte p stroj v bl zkosti jak hokoli tepeln ho zdr...

Страница 111: ...sestupnost k 50 p i 40 C P stroj mus b t um st n na stabiln m rovn m povrchu s nosnost 4 a 5 kg a mus b t zaji t n voln cirkulace vzduchu okolo p stroje Zajist te aby po zapnut p stroje dn materi ly n...

Страница 112: ...vermelde voeding lader Stel de NanoPhotometer niet bloot aan sterke magnetische elektrische velden water chemische stoffen of andere vloeistoffen zoals zware regen of vocht Plaats het instrument niet...

Страница 113: ...ochtigheid niet condenserend 80 en tot 31 C lineair afnemend tot 50 bij 40 C Het instrument moet op een stabiel vlak oppervlak worden geplaatst dat 4 5 kg kan dragen en moet zo worden geplaatst dat lu...

Страница 114: ...que le bloc d alimentation chargeur fourni N exposez pas le NanoPhotometer de puissants champs magn tiques lectriques de l eau des produits chimiques ou d autres types de liquide par exemple de forte...

Страница 115: ...emp ratures de 10 40 C si vous utilisez le chauffage de la cuvette la plage de temp ratures est de 10 30 C Si l instrument est soumis des changements de temp rature extr mes il peut s av rer n cessair...

Страница 116: ...rie de v rifications d autodiagnostic Veuillez contacter imm diatement le fournisseur d origine si vous rencontrez des difficult s techniques ou de manipulation d chantillons Remarque si cet quipement...

Страница 117: ...die integrierte Batterie explodieren kann Nicht in der N he von W rmequellen aufstellen bzw verwenden insbesondere keinen Temperaturen ber 60 C aussetzten Betreiben sie das NanoPhotometer nicht auf Ih...

Страница 118: ...er t sollte so positioniert sein dass im Falle eines Notfalls der Stromstecker leicht identifiziert und entfernt werden kann Beim Transport des Ger tes nicht am optionalen Display oder Hebelarm tragen...

Страница 119: ...forti campi elettrici e o magnetici acqua sostanze chimiche o qualsiasi tipo di liquido ad esempio pioggia fitta o umidit Non porre lo strumento sul fuoco poich pu gonfiarsi o esplodere la batteria N...

Страница 120: ...su una superficie stabile e orizzontale in grado di sostenere un peso di 4 5 kg e tale da consentire la libera circolazione dell aria intorno allo strumento Assicurarsi che quando lo strumento acceso...

Страница 121: ...tarczonego w zestawie i zgodnego ze specyfikacj zasilacza lub adowarki Nie wolno nara a urz dzenia NanoPhotometer na dzia anie mocnych p l magnetycznych i elektrycznych wody chemikali w ani jakichkolw...

Страница 122: ...rzewanie kuwety zakres ten wynosi 10 30 C W przypadku ekstremalnych zmian temperatury mo e by konieczne zr wnowa enie temperatury urz dzenia W tym celu nale y je wy czy i w czy ponownie po osi gni ciu...

Страница 123: ...technicznych lub z pr bk nale y niezw ocznie skontaktowa si z pierwotnym dostawc urz dzenia Uwaga Eksploatacja urz dzenia w spos b niezgodny ze specyfikacj lub w warunkach niesprzyjaj cych bezpiecznem...

Страница 124: ...s intensos ni tampoco a agua productos qu micos o ning n otro tipo de l quido como lluvia o humedad elevada No arroje el instrumento al fuego pues podr a inflamarse o explotar la bater a No lo guarde...

Страница 125: ...condensaci n del 80 y hasta 31 C con reducci n lineal hasta 50 a 40 C El instrumento debe colocarse sobre una superficie estable y plana que pueda soportar 4 5 kg y que permita que el aire circule con...

Страница 126: ...hotometer f r starka magneter elektriska f lt vatten kemikalier eller n gon typ av v tska som kraftigt regn eller fukt L gg inte instrumentet i eld det kan d sv lla och explodera batteriet F rvara int...

Страница 127: ...rande 80 och upp till 31 C linj rt minskande till 50 vid 40 C Instrumentet m ste placeras p en stabil j mn yta som kan b ra 4 5 kg och till ter att luft fritt kan cirkulera runt instrumentet Kontrolle...

Страница 128: ...xcessivas gua produtos qu micos ou qualquer outro tipo de l quido como a chuva forte ou a humidade N o submeta o instrumento a fogo dado que pode dilatar ou explodir bateria N o guarde nem use o instr...

Страница 129: ...igue o instrumento e volte a ligar depois de alcan ar o equil brio t rmico 2 3 horas A humidade relativa m xima n o condensa o de 80 e at 31 C reduzindo progressivamente at 50 a 40 C O instrumento dev...

Страница 130: ...130 Nota Se este equipamento for utilizado de forma n o especificada ou em condi es ambientais n o adequadas para o funcionamento em seguran a a prote o proporcionada pelo equipamento pode ficar comp...

Страница 131: ...say 61 Biuret Assay 61 Blank Control 8 95 Bradford Assay 61 Buttons 30 C Cleaning 105 Compatibility 23 Solvent 41 Concentration 82 Calculations 85 Measurements Protocol 82 Connecting Cable 14 Connecti...

Страница 132: ...Protocol 70 On Off Switch 9 10 11 12 13 P Parameter area 31 PDF 35 Positioning 22 Collocazione 120 Instalaci n 125 Plaatsen 113 Placering 127 Platzieren 117 Posicionamento 129 Positionnement 115 Umiej...

Страница 133: ...alibration Test 99 Side Tab Bar 31 Software Reset 101 Update 101 Solvent Compatibility 41 Standard Curve 86 Calculations 89 Measurements Protocol 86 Standard Solution 15 Stored Methods 91 Stored Resul...

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