NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
44
4.
N
ANO
P
HOTOMETER
®
A
PPLICATIONS
The NanoPhotometer
®
comes with pre-programmed applications as well as the ability to create
custom applications. Any application method can be selected by tapping the icon once or
clicking on the icon (computer based software).
Note
: Within each method selection of nanovolume or cuvette mode (NP80/C40) is possible.
The “Change to Cuvette” or “Change to NanoVolume” button is located at the bottom of the
parameter area.
N
UCLEIC
A
CIDS
M
ETHOD
O
VERVIEW
Nucleic acids in solution absorb light with a peak in the ultraviolet region of 260 nm. For
determination of nucleic acid concentration in solution the absorbance at wavelength 260 nm is
used along with the Beer-Lambert law. In addition to calculating concentrations of nucleic acids,
absorbance measurements are also useful for estimating purity of nucleic acids by calculating
the 260/280 nm and 260/230 nm ratios. Further, it is possible to determine the degree of
labeling of nucleic acids with probes including fluorescent dyes.
Sample Control™ gives useful information about sample conditions. It recognizes air bubbles,
sample impurities, turbidity, lint residues and potential contaminations. If Sample Control™
detects any inconstancy an alert icon
is shown in the result/table area. A click on the alert
icon shows additional information about the inconstancy.
M
EASUREMENT
P
ROTOCOL
1. Select the Nucleic Acids icon on the home screen.
2.
To change between NanoVolume and cuvette application (NP80/C40
only) use the Change to Cuvette/NanoVolume button below the
parameter area.
3. To change the nucleic acid type click on dsDNA and a list with available options opens on
the right side.
Options are: dsDNA, ssDNA, RNA miRNA, miRNA Sequence, Oligo, Oligo Sequence and
Custom (see Table 1).
• The miRNA sequence and Oligo sequence options allow the sequence to be entered
and the extinction coefficient will be automatically calculated. A minimum of 4 bases is
required.
• Custom is set to 50 as default and allows the extinction coefficient (1/
Ɛ) to be manually
