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10. Software STEVE
Single-frame image acquisition
How to do a single-frame image acquisition
A single frame image acquisition is an instantaneous measurement that results in a unique image of the object.
To perform a single shot, click on the 3D button or on the Single Shot from the Microscope menu. The mounting medium refractive index selection
dialog will appear (see section "How to select the mounting medium’s refractive index" previously explained) and the acquisition will start once a
refraction index is selected (Figure 26).
At the end of the acquisition, STEVE will enable to digitally stain the data and interactively explore the cell and its subcomponents. Further down,
we explain the exact steps on how to achieve this. Please refer to “How to digitally stain cells with STEVE”.
Figure 26 Acquisition type dialogue window
