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ADAM-MC2, A New Standard of Automated Cell Counter

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Introduction

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General Operation

Counting cell

Correct volume

Low volume

[Sample loading error]

Be cautious of loading the correct volume of the sample into AccuChip.

The instrument will not detect low or high sample volumes.

!

WARNING

Avoid bubbles which may negatively affect the result.

!

CAUTION

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Содержание ADAM MC2

Страница 1: ...Instruction Manual A New Standard of Automated Cell Counter Copyright NanoEnTek Inc All rights reserved ADAM MC2 ...

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Страница 3: ...Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEnTek America Inc 240 Bear Hill Road Suite 101 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 The information in this manual is described as accurately as possible Firmware and software changes and updates may change without prior consent or notification Copyright 2018 by Nan...

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Страница 5: ...fety symbols Warnings Technical Support General Operation Introduction Sample preparation Counting cell Measure Run sample Result analysis Result analysis Error code Data Data list Edit Image Mail Power off Lock Power off Getting Started Environmental requirements Power on and initial display Error messages during booting Count setting Product Description Front view of ADAM MC2 Rear view of ADAM M...

Страница 6: ...2 Introduction General Description The ADAM MC2 an automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using AccuStain Solution ...

Страница 7: ...method To address these problems NanoEnTek has developed the ADAM MC2 which is based on a fluorescent microscopy technique for counting cells The ADAM MC2 utilizes sensitive fluorescence dye staining LED optics and CMOS detection technologies to make the cell analysis more accurate and reliable To count cells using ADAM MC2 the cells are mixed with a Propidium Io dide PI stain and directly pipette...

Страница 8: ...er to measure the total concentration of cells the plasma mem branes of all the cells must be disrupted to stain all the Nuclei with PI The process of disrupting and staining is achieved by treatment with AccuStain Solution T In the second solution live cells remain intact and are not stained Only the non viable cells are stained and detected After treatment the prepared cells will be loaded into ...

Страница 9: ...oes not cover in transit damage Upon receipt store AccuChip at room temperature AccuStain Solution should be stored at 2 8 C Item Quantity Main device 1 Instruction manual 1 USB hub 1 Wifi dongle 1 Power cord 1 Adapter 1 AccuChip Kit 1 Calibration Bead 1 Item AccuChip2x Kit Cat No AD2K 200 AccuChip4x Kit Cat No AD4K 200 AccuStain Solution Cat No ADR 1000 Disposable Chip 200pcs 2 channel 200pcs 4 c...

Страница 10: ...nserted and ejected Power Power on off LCD Display processes and results START Performs all procedures of automatic counting LOCK Protects the alignment of stage from external shock when ADAM MC2 is being moved Lock ADAM MC2 before turning it off or moving it EJECT Ejects the slide holder from ADAM MC2 Functions as unload Product Description Door Power LCD ...

Страница 11: ... The rear view showing various parts of the ADAM MC2 Port Description USB Port Port for software update and save the data Power Plug Connects ADAM MC2 power cord to wall outlet Product Description USB Port PC Port Power plug Do not use the PC port This port does not recognize USB CAUTION ...

Страница 12: ... 7 Ensure minimal contact with dust or other airborne particles 8 Allow a 10 cm 4 inches minimum space around the device for proper airflow 9 Do not place any objects on the device 1 Check the connection of ADAM MC2 and power cord 2 Press the power button for 2 3 seconds If you get an error message please contact your local distributor or sales nanoentek com If booting is successful and no errors ...

Страница 13: ...lure of X axis sensor 0x00007000 Failure of Y axis sensor 0x00008000 Failure of Z axis sensor 0x06000000 Failure of Locking module sensor Getting Started It appears when booting is not working properly Turn off main power and restart device If this message still appears after restarting contact your local distributor or sales nanoentek com ...

Страница 14: ...et the AccuChip according to you are using Cell size Set the minimum and maximum size of cell Dilution factor When diluting sample set the Dilution factor Factor values for the AccuStain Solution is already applied CAUTION 2Ch 4Ch Accuchip 4Ch 2Ch Accuchip Cell size Min 5 Max 80 Dilution factor T1 N1 T2 N2 1 0 ...

Страница 15: ...omated cell count using the ADAM MC2 Please check the procedure of sample preparation and testing below For more detailed information please refer to the next page 2 Load the mixed sample Then wait 1 minute for the sample settling 1 Mix the sample with AccuStain Solution 3 Insert AccuChip Get the result AccuStain Solution 50 μL Sample 50 μL OR Result 2 channel 23μL 4 channel 13μL T channel Total c...

Страница 16: ...g 1 Add 50 µL of your sample to 50 µL supplied AccuStain Solution N 2 Voltex the tube vigorously 3 Load 23 µL sample mixture to the AccuChip on N channel Then wait 1 minute for the sample settling Total Cell Non viable Cell 1 Add 50 µL of your sample to 50 µL supplied AccuStain Solution T 2 Voltex the tube vigorously 3 Load 13 µL sample mixture to the AccuChip on T1 or T2 channel Then wait 1 minut...

Страница 17: ...2 T1 N1 T2 N2 General Operation Counting cell Correct volume Low volume Sample loading error Be cautious of loading the correct volume of the sample into AccuChip The instrument will not detect low or high sample volumes WARNING Avoid bubbles which may negatively affect the result CAUTION ...

Страница 18: ... slide The instrument will not detect if slides are inserted incorrectly See pictures below for proper insertion WARNING PleaseinsertorremovetheAccuChipwhentheslideholderisfullyejected Whenthetestisfinished pleaseremovetheAccuChipfromtheslideholder CAUTION O X X X Correctly inserted Upside down inserted Not fully inserted Wrong end inserted ...

Страница 19: ...d Cell Counter 15 Introduction Measure Run Sample Start counting process by pressing START It may take about 2 minutes longer for auto focus at the initial test While the test is in progress you can check the cell images of each channel ...

Страница 20: ... N1 5 50E5 50 HeLa_100μM H2O2 T2 2 20E6 N2 5 50E5 75 The viability will be automatically calculated by the ADAM MC2 software after each measurement of the total cells and the non viable cells First the total cell number and second non viable cell number were measured and then the cell viability is calculated as subtracting non viable cell counting numbers from total cell counting 1 10E6 1 10 X 10 ...

Страница 21: ...rs are over 50 of total counting frame O Cells are more than 4X10 cells mL H Cells are more than 2X10 cells mL L Cells are less than 4X10 cells mL U Cells are less than 5X10 cells mL Error frame Frame with error that contains cells whose diameter is larger than 100μm When this error shown in result window please check the image 4 5 6 6 ...

Страница 22: ...ay the data of the selected date EDIT View and edit the data Multiple data can be edited with the same settings IMAGE Check the cell images of each channel SAVE Save the selected data to USB PDF Excel Image DELETE Delete the selected data MAIL Send the Excel PDF and Image files of selected data to e mail ...

Страница 23: ...scription Sample Edit the sample name Cell size graph View the cell size graph each channel T N Cell size setting Set the min max size of the cell Dilution Factor Set the dilution factor of sample Factor values for the AccuStain Solution is already ap plied Frame graph View the counted cell number each frame ...

Страница 24: ...MAGE Control buttons Description Channel Select a channel Original Check the original image Counted Check the counted cell image Frame Select a frame number of the channel Zoom in out Zoom in and out to check the cell image ...

Страница 25: ...ail address from the list to send the Excel PDF and Image files of selected data The e mail address that received the files remains in the list Mail Address If you want to receive files by new e mail please enter the e mail address Send Send the files to a selected e mail address Files can be sent to only one e mail at a time ...

Страница 26: ...itions in the setting tap before counting Refer to page 10 for more information Wifi Set the wifi to use the e mail function Mail Do not change the setting in mail Update Firmware or Software update through the USB Date Time Set the current date and time Capacity Check remaining capacity ...

Страница 27: ...are the USB with update file 2 Insert the USB 3 Click the UPDATE button If connection error occurs please contact a laboratory facility manager CAUTION The AdamUpdate foldermustexistintherootpathoftheUSBfolder ADAM MC2canbeupdatedonlywhenthefirmwareorsoftwarefileexistsin the AdamUpdate folder The ADAMMC2 eve fileshouldbeinthe AdamUpdate folder Do not rename the AdamUpdate folder The folder name sh...

Страница 28: ...ere is no operation for 3 minutes the lock function will be activated automatically Whem the device is locked the screen will be changed as shown below If you press the power button 2 3 seconds then Slide to shut down your PC message will appear Slide down the screen to turn off the power ...

Страница 29: ...C2 has no replacement of consumable materials 3 Please clean the exposed surface of ADAM MC2 frequently or before testing using a soft cloth and isopropyl alcohol or deionized water Dispose of wipes in an appropriately labeled solvent contaminated waste container CAUTION Maintenance and cleaning Maintenance and cleaning ...

Страница 30: ...ing the cells When error message is shown For information on each error message see page 17 When frames with errors are over 50 of total counting frame Error message E Check the suspension of cells if all cells are fully dissociated into single cells If contaminants except cells are found prepare sample again High concentration of cells Error message H Over detection range Error message O Check if...

Страница 31: ... Use of fittings or spare parts supplied by anyone other than NanoEnTek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection items being returned must be insured against possi ble damage or loss NanoEnTek cannot be responsible for damage incurred during shipment of a repair instrument It is recommend that you save th...

Страница 32: ...2x10 cells mL 25 50 sec test DC12V 5A 4 X 4W Green LED CMOS camera Excitation filter Dichroic filter Emission filter 7 Kg 227 276 270 mm IPX0 23 µL test AccuChip 2X 13 µL test AccuChip 4X 8 6 µL test AccuChip 2X 3 4 µL test AccuChip 4X 12 5 mL Propidium Iodide PI Total cells T non viable cells N 0 30 C 2 8 C Temperature Humidity Altitude Temperature Humidity Operating environment condition Transpo...

Страница 33: ... mL AccuStain Solution T 2 12 5 mL AccuStain Solution N 1 AD4K 200 AccuChip4X Kit 200 pcs AccuChip 4X 1 12 5 mL AccuStain Solution T 2 12 5 mL AccuStain Solution N 1 ADR 1000 AccuStain 12 5 mL AccuStain Solution T 4 12 5 mL AccuStain Solution N 2 ADB 500 ADAM Calibration Bead 5 mL Calibration Bead 1 AccuChip 2x please consult your distributor or manufacture for availability ...

Страница 34: ... objects especially metallic into the air vents of the instru ment as this could result in electrical shock personal injury and equip ment damage Always set the main switch on the power supply unit to OFF before con necting the power cord to the wall outlet To avoid a potential shock hazard always connect the grounding termi nal of the instrument and that of the wall outlet properly The power cord...

Страница 35: ...rements Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standard for the US and Canada LED USB Connection When this crossed out wheeled bin symbol is attached to a product it means the product is covered by the European Directive 2012 19 EU All electrical and electronic products should be disposed of separately from the munic...

Страница 36: ...person Cover Do not remove cover or dissemble case There are no adjustable components inside the instrument If a malfunction is found refer to an authorized service person Manual Do not attempt to service the equipment This manual is only available in English Failure to heed warnings may result in injury to service provider or operator Sample handling Wear personal protective equipment during samp...

Страница 37: ... Technical resources including manuals FAQs etc Technical support contact information Additional product information and special offers NanoEnTek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEnTek America Inc 240 Bear Hill Road Suite 101 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 Email sales nanoentek com Website ww...

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Страница 40: ...i Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEnTek America Inc 240 Bear Hill Road Suite 101 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 Email sales nanoentek com Website www nanoentek com ADAMMC2 NESMU AMC2 001E V 0 0 ...

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