12
Introduction
T1
N1
T2
N2
When you load of the sample mixture to the AccuChip, please be careful
not to make bubbles.
!
NOTE
Concentration out of this range will result in errors.
Refer to page 17 for more information about errors.
!
NOTE
Counting cell
General Operation
Sample
preparation
3. Thoroughly mix the cell pellet by vortexing.
4. Check visually if any cell clumps or agglomerates are remained.
[AccuChip 2x]
[AccuChip 4x]
Total Cell
Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
T
.
2) Voltex the tube vigorously.
3) Load
23 µL
sample mixture to the
AccuChip on
T
channel. Then, wait
1 minute for the sample settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
N
.
2) Voltex the tube vigorously.
3) Load
23 µL
sample mixture to the
AccuChip on
N
channel. Then, wait
1 minute for the sample settling.
Total Cell
Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
T
.
2) Voltex the tube vigorously.
3) Load
13 µL
sample mixture to
the
AccuChip on
T1
or
T2
channel.
Then, wait 1 minute for the sample
settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
N
.
2) Voltex the tube vigorously.
3) Load
13 µL
sample mixture to the
AccuChip on
N1
or
N2
channel.
Then, wait 1 minute for the sample
settling.
Total
Total
T/N2
T/N1
Non-viable
Non-viable
1.
Cultivate the required number of cells.
2.
Add an appropriate volume of growth media or PBS to dilute to a final
concentration of 5 x 10 cells/mL to 4 x 10
cells/mL.
4
6