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Direct Detect® Spectrometer User Guide

2

2 

Components Supplied

The Direct Detect® system includes the following components:

Figure 1.  Components supplied

1A

1B

1C

1D

1E

1F

1G

1H

1A

Direct Detect® spectrometer

1B

Instrument power adapter with 4 power cord/plug configurations (EU, UK, US, Japan)

1C

Data cable (crossover, 10Base-T Ethernet with RJ45 connectors)

1D

Dell® Netbook computer with Direct Detect® Software installed

1E

Computer power adapter with world-wide plug adapter

1F

Netbook stand

1G

Direct Detect® Assay-free cards (package of 50)

1H

Direct Detect® spotting tray
Desiccant pack replacement (1, not shown)
Torx® TX20 screw driver for replacing the IR source (not shown)
Direct Detect® Spectrometer Quick Start Card (not shown)

Содержание DDHW00010-WW

Страница 1: ...User Guide Direct Detect Spectrometer...

Страница 2: ...2014 EMD Millipore Corporation Billerica MA U S A All rights reserved 00000833 Rev D 05 14 Notice The information in this document is subject to change without notice and should not be construed as a...

Страница 3: ...uidelines 11 8 1 Buffer components 11 8 2 Materials Required but Not Supplied 11 9 Direct Detect Assay free Card and Sample Preparation 12 9 1 Direct Detect Assay free Card 12 9 2 Sample Preparation 1...

Страница 4: ...ption of Instrument Validation Tests 36 13 5 Running the Instrument Validation OQ and PQ Tests 39 13 6 Storage 39 14 Installation Operation and Process Qualification Protocol Optional 40 14 1 Installa...

Страница 5: ...asures amide bonds in protein chains accurately quantifying an intrinsic component of every protein without relying on amino acid composition dye binding properties or redox potential Protein concentr...

Страница 6: ...figurations EU UK US Japan 1C Data cable crossover 10Base T Ethernet with RJ45 connectors 1D Dell Netbook computer with Direct Detect Software installed 1E Computer power adapter with world wide plug...

Страница 7: ...ter 1 Subchapter J i Read the documentation h Catalogue number f Serial number M Manufacturer C CE conformity marking Reference Declaration of Conformity for specific directives Do not discard with co...

Страница 8: ...poration for use with the Direct Detect system Use of other power supplies may damage the system Minimize power draw from other instruments on the same power circuit Do not use with flammable or explo...

Страница 9: ...onnected to the same electrical circuit as the Direct Detect spectrometer Vibration Do not locate the Direct Detect spectrometer near vibration sources centrifuges vortexes ventilation hoods air condi...

Страница 10: ...nto the power port of the PC but do not plug the computer into a power outlet at this time 6 5 Unpacking and Setting Up the Direct Detect Spectrometer Remove all components from shipping boxes and lay...

Страница 11: ...should be left on and powered up at all times 6 5 2 Initializing the Direct Detect Spectrometer 1 Refer to the Direct Detect Software User Guide for instructions on starting up the software and loggin...

Страница 12: ...indicator light 5G Card insertion light 7 1 2 The Direct Detect Automated Card Holder The automated card holder 5C accepts only 4 sample Direct Detect Assay free cards The top of the card holder modu...

Страница 13: ...e When data acquisition is occurring the light is bright green The abbreviation TKD means Take Data 6D ACC LED The yellow ACC LED indicates that the network is accessing the optics During transition o...

Страница 14: ...dapter and power cord The POWER port is for extra low voltage connectors Use only power cords supplies that have been supplied by EMD Millipore Corporation 6K Kensington lock slot The lock slot is an...

Страница 15: ...ntaining more than 5 glycerol SDS and or Tween 20 surfactant should be avoided due to the excessive time required to achieve dryness The instrument comes with preloaded protein calibration files prepa...

Страница 16: ...t is reserved for the blank measurement The blank is the buffer solvent used to resuspend the sample but contains no protein or lipid detergent One or more of the remaining card positions are used for...

Страница 17: ...0 mM Tris 50 L of 1 mg mL protein in 50 mM Tris 50 mM Tris 50 L of 1 mg mL protein in PBS with 9 5 mM Tris PBS with 9 5 mM Tris Add 450 L of 1X PBS or exchange buffer 500 L of 0 1 mg mL protein in PBS...

Страница 18: ...the icon on the Netbook desktop Figure 9 Direct Detect Software desktop icon Each instrument user can be assigned a unique name and optional password Figure 10 NOTE If 21CFR11 mode is enabled a passwo...

Страница 19: ...free card into the instrument However for nonflammable samples the Direct Detect spectrometer has a built in drying system that can automatically dry the sample to the point where it will give an accu...

Страница 20: ...ct Detect system comes with three preloaded protein calibration methods These pre existing methods may be used for all future measurements however for accurate quantification we recommend preparation...

Страница 21: ...rd holder the software will prompt you to remove it 10 3 2 Calibration The Calibration Method Wizard Options menu Generate Calib Curve is used to create or modify calibration method files that will be...

Страница 22: ...4 positions on the card have been read the instrument will sound a tone The card will rise to the initial insertion position and can be removed CAUTION Do not remove card during the reading To stop a...

Страница 23: ...tests prior to changing the connections refer to section 14 2 The IP Address Change Utility is located in C directdetect IPAddressChanger This utility assists in the transition between direct connect...

Страница 24: ...Connection IMPORTANT This section assumes that the Direct Detect instrument is currently connected directly to the computer using the supplied crossover 10Base T cable with RJ45 connectors 1 If the re...

Страница 25: ...in the DOWN position refer to Figure 21 7 Select the Next button to proceed 8 Run one of the standard RJ45 Cat5e Ethernet cables between the ETH LAN port on the rear of the instrument 6B in Figure 6...

Страница 26: ...ion IMPORTANT This section assumes that the Direct Detect instrument and Netbook computer are currently connected to your intranet using two standard RJ45 Cat5e Ethernet cables 1 Run the IP Address Ch...

Страница 27: ...rument 6B in Figure 6 and the network LAN port on the computer 3B in Figure 3 8 Reconnect power to the instrument 9 Wait for the instrument to initialize and then select the Test Connection button on...

Страница 28: ...CPIP in the search field Select the View network connections option in the control panel section Figure 26 Accessing network connections in the Windows 7 operating system 2 Select your network connect...

Страница 29: ...ct Detect software section 11 2 Figure 28 IP address and subnet mask settings for direct connection OR For a connection between the Direct Detect instrument and the computer via your intranet consult...

Страница 30: ...ypically the same at any given time and indicate the following Green Instrument is in proper operating condition flashing green on the instrument indicates stand by mode Yellow Instrument is initializ...

Страница 31: ...meter initialization is completed several minutes IR source has not yet reached its operating temperature The warm up takes about 7 minutes As soon as the IR source has reached its operating temperatu...

Страница 32: ...er outlet Short circuit in the adapter or power cable A short circuit is accompanied by a ticking sound in the power adapter Unplug the power cord immediately and contact Technical Service Spectromete...

Страница 33: ...validation icon is red An instrument validation test has failed To find out which instrument validation test has failed click on the instrument validation icon to open the instrument validation dialog...

Страница 34: ...fe span of 44 000 hours As the end of this life span is approached the hardware diagnostics icon turns yellow When you click on the hardware diagnostics icon Figure 30 the hardware diagnostics dialog...

Страница 35: ...igure 33 Removing the IR source IR source Source plug IR source Torx screws Alignment groove 4 Loosen the two mounting screws on the IR source Figure 33 with the Torx screw driver 5 Disconnect the gre...

Страница 36: ...e that the alignment pin fits into the alignment groove Figure 34 8 Secure the source by fastening the two Torx screws Figure 35 9 Plug the green source plug into the SRC female connector Figure 35 Re...

Страница 37: ...he hardware diagnostics icon turns green If the instrument fails refer to section 5 1 in the Direct Detect Software User Guide 13 2 Replacing the Desiccant Excessive humidity inside the spectrometer m...

Страница 38: ...d as soon as possible Replace the desiccant as follows 1 Unplug the power plug 2 The desiccant is accessible from the rear side of the spectrometer Loosen the 4 Torx screws Figure 32 with the Torx scr...

Страница 39: ...r plate and fasten the 4 Torx screws again Figure 32 7 Plug the power plug into the power outlet again After the new desiccant removes the moisture from the chamber approximately 30 minutes the instru...

Страница 40: ...roceed under the expired PQ condition The PQ test protocol consists of the following three tests Signal to noise test The signal to noise ratio determines the sensitivity of the spectrometer by calcul...

Страница 41: ...iation 0 50 cm 1 0 20 cm 1 Overall Test Result PASSED Date and Signature Date and Signature Figure 39 PQ test report 13 4 2 Operational Qualification Test OQ The Operational Qualification test checks...

Страница 42: ...ocol Company Operator Instrument Type Optics Configuration Accessory Instrument Serial Number Instrument Firmw are Version OPUS DB Version Overall Test Result Test expires Test Date Time Test Spectra...

Страница 43: ...ification PQ Run Test button 1 in Figure 41 The test takes about 5 minutes Figure 41 Running OQ PQ tests 2 1 Instrument validation icon and OQ PQ Initiator 4 To run the Operational Qualification OQ te...

Страница 44: ...m starts the initialization sequence and that successful completion is indicated by the status indicator light 5E in Figure 5 turning green Document results on the IQ OQ PQ Report Summary page found i...

Страница 45: ...ics in the Direct Detect Software User Guide Correct the problem then click on the hardware diagnostics icon to verify that all of the check marks are green Select the save button to save the hardware...

Страница 46: ...on the Performance Qualification PQ Run Test button 1 Run this test regardless of the test expiration date A report displaying the PQ results will be generated and stored in C DirectDetect Validation...

Страница 47: ...mple Result tab of the main screen 8 Measure the BSA standard concentration using a UV reader at 280 nm DO NOT use standard curves built into the UV reader Use the sequence based extinction coefficien...

Страница 48: ...trument cross over cables Verify Software communication Direct Detect Software launches successfully Login successful Instrument detected 14 2 Instrument Hardware Diagnostic All functions have green c...

Страница 49: ...omplies with EN IEC 61010 1 08 2002 Safety requirements for electrical equipment for measure ment control and laboratory use EMI regulations Complies with EN IEC 61326 05 2004 Electrical equipment for...

Страница 50: ...uter with power adapter Ethernet crossover cable Netbook computer stand 50 pack of Assay free cards spotting tray spare desiccant bag Torx screw driver Quick Start Guide Power adapters are configured...

Страница 51: ...r up to date worldwide contact information You can also visit the tech service page on our web site at www millipore com techservice 18 EC Representative Millipore UK Ltd Fleming Road Kirkton Campus L...

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