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Milestone s.r.l.

MM098-002

– LOGOS ONE – Operator Manual

117

TISSUE PROCESSING SELECTION GUIDELINES

Washing

Ethanol 1 min

FRESH RENAL BIOPSIES

Soaking in Formalin up to 30 min.

Heating in Formalin

5 min. ramping time to 50°C

10 min. holding at 50°C

Ethanol 100% - 10 min total time.

Isopropanol 100% - 10 min total time.

WAX 15-20 min.

PARTIALLY FIXED
TISSUE BLOCKS

FIXED TISSUE

10/

20’: Small biopsy

30’:

3mm

60’:

5mm

Heated Fixation

complete phase

FRESH TISSUE

30’:

Small biopsy

1 hr:

3mm

1.5 hr: 5mm

2/3 hrs:5mm breast/fatty tissues

Soaking in Formalin

with agitation

Rinsing in Ethanol

Ethanol

JFC

Isopropanol

Routine

method

Fatty

tissues

ProWave
(o JFC)

One step

routine

Isopropanol

WAX

Содержание LOGOS One

Страница 1: ...LOGOSOne All in one RapidTissue Processor OperatorManual MM098 MILESTONE H E L P I N G P A T I E N T S...

Страница 2: ......

Страница 3: ...ou to read carefully this operator manual and to keep it in reach for convenient and fast consulting For any possible clarification or any request for assistance please contact either our Representati...

Страница 4: ...2 10 Log in and log out 24 2 10 1 Create a new user 25 2 11 Set system date and time 27 2 12 Set language 27 2 13 Load LOGOS ONE with wax 28 2 13 1 Wax heating setting 30 2 14 Load LOGOS ONE with reag...

Страница 5: ...95 6 2 2 Print process 97 6 2 3 Send process to customer support Milestone 98 6 2 4 Change settings 99 6 2 5 Other functions 99 6 3 Reagents Log and Events Log from the terminal to the USB key 100 7...

Страница 6: ...st electric shock Be sure to follow the instruction to prevent accidents resulting from electric shock Biohazard be careful when you execute the procedure marked with this symbol danger of biohazard c...

Страница 7: ...wax infiltration The Milestone LOGOS ONE must be operated exclusively with the reagents listed in section Compatible reagents 1 7 1 3 Technical specifications CODE 61500 R Power supply input 230V 50Hz...

Страница 8: ...defined programs I O Interface 2x USB port 2 0 1x LAN Ethernet 10 100 Mbps RJ 45 1 4 Transportation and storage conditions Temperature 20 C up to 60 C Humidity up to 80 up to 50 at 40 C 104 F non cond...

Страница 9: ...not remove the instrument accessories panels or cover Only authorized and qualified SERVICE personnel may repair the instrument and access the instrument s internal components It is important that nor...

Страница 10: ...tion WAX Histology grade Alcohol water 60 40 solution Flushing Xylene Formalin buffered or unbuffered Alcohol water 70 30 solution Milestone Prowave solution Isoparaffin Milestone MileGREEN solution E...

Страница 11: ...tal managed of equipment is mandatory It is necessary to return the used equipment to the distributor or to inquire about the presence of a local empowered system for collection and disposal of WEEE T...

Страница 12: ...vered in a wood box with these dimensions Width 920mm 36 2in Height 1320mm 52in Depth 1500mm 59in The space dimensions required to locate the unit are the following drawer and covers opened Width 1100...

Страница 13: ...LOGOS ONE COMPLETE PACKAGE 61500 R 230V 50Hz 2000W 61503 R Starter Kit LOGOS ONE 61505 R LOGOS ONE COMPLETE PACKAGE 61501 R 230V 60Hz 2000W 61503 R Starter Kit LOGOS ONE The LOGOS ONE COMPLETE PACKAG...

Страница 14: ...items CODE DESCRIPTION Q ty IMAGE 66105 Rack Transfer Kit 1 66165A 210 Split Rack 210 cassettes 2 62348 Antiscratching Spatula to remove wax traces 2 62160 USB Data Traveler containing the LogViewer S...

Страница 15: ...ITY on the RIGHT To open the MAIN CAVITY cover push the button then rotate the handle clockwise then flip up the cover To open the WAX CAVITY cover push the button then rotate the handle clockwise the...

Страница 16: ...s Length 3m 110 External diameter 69mm 2 72 Internal diameter 60mm 2 36 Insert the exhaust tube CODE 61377 on the proper outlet back panel Use the metal cable clamp CODE 50051 to fix the hose to the e...

Страница 17: ...e electronic noise originated by other instruments supplied with the same line Power supply line wires their size and distance from the switch board used in the laboratory are compliant with the label...

Страница 18: ...xternal alarm connector 3 Ethernet LAN plug 4 UPS side supply controller power supply input 5 UPS supplier socket energy output to local ups unit 6 Main power supply As separable power cord it is stri...

Страница 19: ...rotect the unit from power supply failure In fact in case of power failure user cannot remove samples from LOGOS ONE nor do any other operations For this reason Milestone s r l does NOT suggest this t...

Страница 20: ...power supply In this case LOGOS ONE stops working and the standard power failure procedure that puts tissues in safety is not activated For this reason we highly recommend to install a UPS module on a...

Страница 21: ...er input Maximum current 4A 230V frequency same as local refer to figure below A Connect the UPS power output to the UPS side supply connector type IEC320 C14 refer to figure below B Finally connect t...

Страница 22: ...address and Subnet Mask strongly preferred to DHCP service only port 80 has to be enabled without limitations LAN gateway and DNS server addresses At least 150kb s real transfer rate uploading and dow...

Страница 23: ...er the data required supplied by the Network Administrator For example IP Address 192 168 1 235 Subnet Mask 255 255 255 0 Default Gateway add 192 168 1 6 DNS server address 192 168 1 7 WINS Not requir...

Страница 24: ...ank of 5l or 1US gal MileGREEN for processing of larger fatty tissues 3mm and larger use of Milestone MileGREEN solution is recommended If an isopropanol alternative is used a flushing in isopropanol...

Страница 25: ...E under the USERS settings choosing between Favorites and Main functions see below 2 10 1 Create a new user Go to the Settings screen and select Users to create a NEW USER The USERS setting screen app...

Страница 26: ...riate FUNCTIONS for the NEW USER Pressing the arrow see figure below circle it is possible to choose the appropriate ACCESS LEVEL in the program startup here automatically Ask for USER and PASSWORD Th...

Страница 27: ...operations during the reagent management who replaces the reagents manually 2 11 Set system date and time From the HOME PAGE go into the Settings screen and select Date time see figure below In the ti...

Страница 28: ...Enter in Reag Cons screen a RED icon appears over the WAX CAVITY and the word Replace is RED Select the WAX CAVITY and press Replace reagent button figure below The screen below appears showing the W...

Страница 29: ...of LOGOS ONE and place paraffin in the cavity We suggest to clean the cavity with paper before insert new paraffin In LOGOS ONE it is possible to load solid as well as already molten new WAX It is nec...

Страница 30: ...r from the Main menu select the WAX icon on the status bar right figure below The heating settings screen appears displaying information about the Heating Status and the Cavity Level Main processing c...

Страница 31: ...em malfunction Use only under direct control of Milestone s r l technical support The WAX heating status icon indicates through a change in color the WAX levels WAX Temperature to maintain The reagent...

Страница 32: ...ks and up to 2 layers with 1 US gallons 3 8 liters Do not insert tanks with a capacity lower than 3 8 liters because the reagent will be too much contaminated Do not use tanks higher than 30cm because...

Страница 33: ...STATUS BAR of the HOME PAGE The REAGENTS CONFIGURATION screen appears figure below showing the default settings of the tanks Select the tank in position NUMBER 1 Start set up of the tanks from hose nu...

Страница 34: ...itable for the reagent you want to use in each position in case of position Nr 1 Formalin is suggested as follows press Reagents list from the Reagents configuration screen look to the color and the s...

Страница 35: ...rt the rapid connector in the corresponding position number 1 in this case Milestone suggests to make manually an hole into the original cup of the tank as shown below If you make an hole into the cup...

Страница 36: ...ENTS CONFIGURATION screen select the tank in position NUMBER 1 and press Fill the tank The following message appears click YES The screen on the right appears showing the REAGENT REPLACEMENT PROCEDURE...

Страница 37: ...LOT number When the code is inserted the following screen appears the lot number is shown into the field Press Done to complete the replacement procedure The Lot Code is saved in the Reagents Log und...

Страница 38: ...o the position number 3 see the circle in figure below must be inserted in the position 3 showed by the arrow below The same concept is required for all the other tanks It s required to insert the tan...

Страница 39: ...ke for a few minutes dispose of waste reagents and follow with two quick rinses in Ethanol Reinstalling of tanks and reagent replacement load tanks with fresh cleaning reagents put the pipes then inse...

Страница 40: ...DEFINED icon 3 Select the CAVITY LEVEL the PROTOCOL the PROGRAM and the BUTTON COLOR to change TEXT and BACKGROUND COLOR Preview is displayed on bottom left 4 Press Save Favourite to save changes 5 Th...

Страница 41: ...n to the Main functions screen If HOME PAGE is set as MAIN FUNCTIONS press Favourites to open the FAVOURITES SCREEN figure below right 2 16 Owner settings This function is available only for SERVICE a...

Страница 42: ...both exhaust fans are activated at the switching ON of the unit Always off both exhaust fans are always OFF even during the processing NOT SUGGESTED Risk of fumes in the laboratory environment Process...

Страница 43: ...is possible to lower or increase volume BUT for safety it is not possible to totally silence it By default the Unit is set up to work with 1 rinsing tank If you want to work with 2 rinsing tanks pleas...

Страница 44: ...er to run a process in two different ways AUTOMATIC mode wax will be automatically pumped from the WAX cavity to the MAIN cavity for the impregnation phase DUAL mode USER is required to MANUALLY move...

Страница 45: ...dripping of formalin or other solutions on the floor or on the LOGOS ONE unit The first rack layer must be blocked in that position Do not insert the layer from the column this will avoid stirrer rota...

Страница 46: ...the most used program Select a Favourite program figure below out of the list of FAVOURITE PROTOCOLS see chapter 2 15 The following screen appears enter the Run ID of the process to be run if required...

Страница 47: ...according to the Favorite protocol selected figure below It is not possible to change the rack of a favourite program because the rack is set in the program so pressing RACK SELECTION you will come b...

Страница 48: ...ELAY for details see chapter 3 2 6 A GO the run is started immediately B GO DELAY start of the run is delayed so that program automatically starts and is completed by the time set by the USER The foll...

Страница 49: ...de is selected the following RUN SUMMARY screen appears displaying all details of the process like RUN DURATION and the COMPLETION TIME Press Start run to run the cycle NOW On completion of first reag...

Страница 50: ...For further information press the arrow on the right of the MAIN CAVITY graph Picture on the right REAGENT TEMPERATURE STIRRER SPEED time DETAILS of the current phase highlighted in blue and RUN DETAI...

Страница 51: ...UTOMATICALLY pumped from the WAX CAVITY right to the MAIN CAVITY left An audio alarm warns when program is over press Unload reagent then Extract the rack After every WAX phase it is suggested to chec...

Страница 52: ...st reagent loading a graph is displayed on the screen with detailed processing information On completion of the process it is necessary to carry out a MAIN CAVITY CLEANING CYCLE for further informatio...

Страница 53: ...urrent phase highlighted in blue and RUN DETAILS of the total program On the bottom left all phases of the program are displayed with the completed or actual in blue To return to the left screen press...

Страница 54: ...rocess AUTOMATIC or DUAL in the MAIN CAVITY without waiting for the end of the WAX phase of the previous process run DUAL mode does NOT require a final MAIN CAVITY CLEANING CYCLE as requested instead...

Страница 55: ...to 210 three layers Press Enter In the example 60 cassettes have been selected so the software automatically sets a process of 2 layers rack and the following posts appear figure below Rack Layer 1 C...

Страница 56: ...rted Therefore when using SPLIT RACK it is possible to force the Cavity Level at 3 when starting a program with Cavity Level 2 SUPERMEGA RACK it is possible to force the Cavity Level at 3 when startin...

Страница 57: ...ions reported above in the chapter 3 2 1 3 2 3 Run two processes simultaneously In DUAL RUN mode described in the chapter 3 2 1 2 an audio alarm warns the operator that the last phase in the MAIN CAVI...

Страница 58: ...ly loaded in the MAIN CAVITY Removal of the rack must be done within 3 minutes Should this not be done the system AUTOMATICALLY pumps WAX in the MAIN CAVITY to preserve samples If the RUN MODE becomes...

Страница 59: ...as described in the chapter 3 2 1 MAIN CAVITY Cleaning to run a cleaning of the MAIN CAVITY as described in the chapter 3 3 3 2 4 Run a program using the STEP START function In the starting screen li...

Страница 60: ...rature OR Holding reagent flushing mix at room temperature By default Pre soaking reagent is selected if you want to keep the specimens immersed in Holding solution select its icon If you leave select...

Страница 61: ...and entering Min and Max fixation times see chapter 2 16 and figure below right when starting a program the system calculates the time necessary to keep samples in HOLDING SOLUTION default reagent Flu...

Страница 62: ...will be possible in the pre soaking or holding phase to add cassettes up to 140 cassettes here 50 cassettes have been selected and so only 90 cassettes is allowed to add to arrive at 140 cassettes Pr...

Страница 63: ...in this case does NOT correspond to the rack layer If you press Set to 3 CUSTOM the process will be executed with 3 levels of reagents and so it will be possible to add cassettes to fill 3 layers of t...

Страница 64: ...It is possible to recall it in every moment pressing the icon on the status bar The screen saver gives very useful information during a program for example when a cavity is free for a new process is...

Страница 65: ...at are visualized in the cleaning page Choose the default program according to the cleaning reagents loaded in the REAGENT CONFIGURATION Only one program can be chosen by default and will be the one t...

Страница 66: ...big routine samples 2 isopropanols are required in overnight run In this case it is suggested to choose GROUP WITH 2 RINSINGS TANK NR TANK REAGENT REAGENT 1 FORMALIN FORMALIN 2 FLUSHING MIX ETHANOL f...

Страница 67: ...ROPANOL 100 8 CLEANING ISOPARAFFIN or XYLENE ISOPARAFFIN or XYLENE 9 CLEANING ETHANOL ETHANOL from 96 to 100 CASE 3 HistosMATE NOT available to clean the rack 3 cleaning tanks required AND Specimens t...

Страница 68: ...PROPANOL 100 7 CLEANING ISOPARAFFIN or XYLENE ISOPARAFFIN or XYLENE 8 CLEANING ETHANOL ETHANOL from 96 to 100 9 CLEANING WATER WATER 3 4 Reagents group and programs The system allows ONLY TO SERVICE P...

Страница 69: ...o the possibility to run the cleaning cycle By default WAX for cleaning is checked for Performed cycles 5 cycles but the Administrator can change the setting in the WAX cleaning window The cleaning cy...

Страница 70: ...t Configuration screen under the WAX cleaning icon number of cassettes processed performed cycles and loading date are RESET to zero but not the EXPIRATION LIMITS in the reagent configuration screen D...

Страница 71: ...lowing screen appears figure below showing on the left all the SAVED PROCESSING FILES sorted by DATE The dates are automatically created by the software Select one date in the example below 4 Sep 2014...

Страница 72: ...098 002 LOGOS ONE Operator Manual 72 File names indicate type of program and cavity where ended FINISH TYPE Date End time M finished in MAIN CAVITY Nothing processing W finished in WAX CAVITY Cleaning...

Страница 73: ...e different saved processing PHASES Heated phases graphs show up to 2 colored lines describing the PRESET TEMPERATURE blue and the REAL TEMPERATURE red output values about the current step In this cas...

Страница 74: ...a program DEFAULT PROTOCOLS the Owner is MILESTONE and USER can only COPY them USER PROTOCOLS the Owner is USER and he can MODIFY DELETE and COPY them if any This function is NOT to create a NEW PROG...

Страница 75: ...PROGRAM to be copied 3 Select the PROGRAM to be copied then press Copy 4 The copy program screen is open 5 RENAME the program using the keyboard and press enter 6 Choose the parameters to be modified...

Страница 76: ...the association between a program and the rack following this logic LAYERS LOGOS ONE CAVITY LEVEL AND RACK 1 NOT PRESENT NOT PRESENT 2 Split RACK 1 140 cassettes Super Mega RACK 1 12 cassettes The max...

Страница 77: ...gram also a favourite program is correlated to one rack that is automatically selected in the favourite program In the following example a favourite program for SuperMega rack SM is set so when the fa...

Страница 78: ...your interest figure below right arrow Press Copy button figure below right rectangle re name the program and modify the fields This function is NOT to create a NEW PROGRAM For this it is necessary to...

Страница 79: ...gram of your interest figure below arrow Press Copy button figure below rectangle re name the program and modify the fields Take care to modify ONLY the Time At Temperature part of the curve Do not mo...

Страница 80: ...n on the STATUS BAR of the HOME PAGE The REAGENTS MANAGEMENT window appears showing the settings and the status of all the reagents loaded in the unit Wax included It is possible to check the reagents...

Страница 81: ...hown on the right side of the windows green square and refers to the REAGENT SETTINGS edited into the Reagent list figure above arrow See chapter 4 1 1 for further clarifications 4 1 1 Reagents settin...

Страница 82: ...N reagent to be used also as RINSING reagent Click on the relative Check boxes to activate the settings described above points 1 2 3 in the chapter 4 1 regarding date of next change number of maximum...

Страница 83: ...for the processed cassettes has a limit of 630 the REAGENTS STATUS icon is green until 419 cassettes one full rack before reaching the limit then turns to yellow from 420 to 629 and then to red at 63...

Страница 84: ...s icon on the STATUS BAR of the HOME PAGE The REAGENTS CONFIGURATION screen appears figure below automatically the expired tank in position NUMBER 2 blinks Select the EXPIRED reagent in this case Flus...

Страница 85: ...rom the drop down menu figure below circle Press Done to confirm and return directly to the REAGENT CONFIGURATION screen Close the tanks holder doors and check the reagent level working label Settings...

Страница 86: ...4 2 1 3 After the replacement the software resets the reagent management count to zero and renames the old RINSING1 reagent as new DEHYDRATION reagent figure below The software renames the other tanks...

Страница 87: ...from the Reagents status icon on the STATUS BAR The icons that are active are colored the inactive are grayed out During the reagents replacement procedure a padlock appears on the WAX tank because i...

Страница 88: ...iminate replace it as follows 1 If WAX reuse life has expired a RED icon appears over the WAX CAVITY REAGENT CONFIGURATION screen and the word Replace is RED Select the WAX CAVITY and press Replace re...

Страница 89: ...open After the replacement the software resets WAX management count to zero and the loading date automatically changes to the current date 3 Wait until the new solid WAX loaded has melted 100 minutes...

Страница 90: ...e safety position The Terminal Computer remains active during the countdown BUT If during this time the main power comes back on the system will restart from the point the program stopped Instead if t...

Страница 91: ...STS AND IS NOT WAX IF NEXT PHASE EXISTS AND IS WAX AVAILABLE IF NEXT PHASE EXISTS AND IS WAX NOT AVAILABLE FINISH UNLOADING FINISH UNLOADING FINISH UNLOADING LOADING NEXT REAGENT LOADING WAX IN MAIN C...

Страница 92: ...with the STEP START function starting from the phase not executed see chapter 3 2 4 for details AFTER THE 20 MINUTES If Reagent Loading Time Out occurs when nobody is in the Laboratory after 20 minut...

Страница 93: ...ase is the first the previous reagent does not exist this is the reason why it is it is necessary that operator stays close to the unit at the beginning of a new process until loading of the first rea...

Страница 94: ...luding all necessary information like the real time and temperature performed by the unit the reagent management and the Events Logs 6 1 Export processing from the terminal to the USB key Insert the U...

Страница 95: ...older Double click on the SetupLogViewer exe icon to install the program The LogViewer software is now ready to work Microsoft NET Framework 4 0 is a prerequisite to this application the setup will in...

Страница 96: ...ails of that process PHASES all details of different phases Click on Show step list for details of the selected STEP green circle GRAPH LINES info and selection For an enlarged view of a processing cu...

Страница 97: ...ummary this print preview includes all phases without graphs with process and unit information Print current phase this print preview includes phase selected with process and unit data Print full proc...

Страница 98: ...of the Print icon from the bar in the upper left of the preview page 6 2 3 Send process to customer support Milestone Click on the icon Send process to customer support green circle to forward an auto...

Страница 99: ...e mail 6 2 4 Change settings Select Settings icon green circle to change the settings configuration All settings are displayed as follows Graph style modify the color of the X Y axes show hide the gr...

Страница 100: ...og The following screen appears where it is possible to export the List of Reagent Movements press Export to copy it After exporting plug the USB in a PC a folder named Logos_ONE_S N is present contai...

Страница 101: ...Milestone s r l MM098 002 LOGOS ONE Operator Manual 101 After exporting plug the USB in a PC a folder named Logos_ONE_S N is present containing the file EventsLog s3db Summarizing the USB may contain...

Страница 102: ...e standards 7 2 After each run Ensure that LOGOS ONE is generally kept clean after each use Do not use abrasives or sharp instruments that may damage external surface etc Clean as soon as possible the...

Страница 103: ...starts automatically 4 At the end of the cycle both the rack and the main cavity are clean from wax and a new process can be started SECOND WAY HistosMATE instrument requested Xylene to clean the rack...

Страница 104: ...ding to lab practices appropriate for that reagent Cleaning procedure perform a rinse in Xylene or Isoparaffin or substitute close tank and shake for a few minutes dispose of waste reagents and follow...

Страница 105: ...REMOVE THE FILTER FROM THE LOGOS ONE MAIN CAVITY 1 Open the cover of the main cavity 2 Remove the pivot from the bottom of the cavity turning the pivot anticlockwise When the cleaning tanks are severe...

Страница 106: ...a brush remove manually any residual from the filter Then rinse it with water 5 Dry the filter with a dry cloth or paper 6 Reinsert the filter in the cavity 7 Reinsert the pivot inside the hole prese...

Страница 107: ...rther details see the chapter 2 8 In administrator screen press Service icon to get Service Screen Press Remote Assistance to start the connection At this point remote service works with your instrume...

Страница 108: ...t max UPS 190X600mm CODE 61851V UPS Holder Weight max UPS 15Kg CODE 66130 Rack for 24 Supermega Cassettes for Pathos Delta Logos and Logos One To enhance the reagents management there is a conversion...

Страница 109: ...ad the cassettes STEP 1 start loading 1 line of cassettes into the center of the rack from one side to the other STEP 2 load a second line of cassettes next to the first STEP 3 load a third line of ca...

Страница 110: ...re filter Fan air flow range 50 55m3 h cube meter per hour DO NOT BLOCK THE FILTER OUTPUT The silicon stopper that you find in the kit is NOT USED in LOGOS configuration The external fume extraction s...

Страница 111: ...the black seal 4 Remove the filter 5 Replace the filter with the new one place the black seal 6 Close the chassis of the carter and screw the six knobs 1 2 3 4 5 6 In case your LOGOS ONE is connected...

Страница 112: ...in the same run The time at temperature pre stored protocols are suited for the most sensitive tissue 2 Specimens with different thickness but all up to 5mm can be simultaneously run While the tempera...

Страница 113: ...he case sponges are used increasing of the Ethanol JFC Isopropanol and WAX processing time by half of the preset time is recommended Use ONLY Histology sponges If the Laboratory is already using parti...

Страница 114: ...ination of tissue cassettes with and without sponge biopsy pads showed that the processing artifacts are a result of water contamination of the heated ethanol step Sponge biopsy pad cassettes have the...

Страница 115: ...size cassette in less than 4 hours PROWAVE WAX 2 PROWAVE is a one step solution that can be used for tissues where overnight fixation time is uncertain since PROWAVE can also act as a secondary fixat...

Страница 116: ...resoaking guidelines PRESOAKING THICKNESS PHASE 1 PRE SOAKING Transplant 15 min 1mm 30 min 2mm 45 min 3mm 1hr 4mm 1hr 15 min 5mm 1hr 30 min 5mm fatty 2 3hr All above are indicative times range for gen...

Страница 117: ...ing at 50 C Ethanol 100 10 min total time Isopropanol 100 10 min total time WAX 15 20 min PARTIALLY FIXED TISSUE BLOCKS FIXED TISSUE 10 20 Small biopsy 30 3mm 60 5mm Heated Fixation complete phase FRE...

Страница 118: ...e to use of paper wraps that restricts the flow of reagents For larger biopsies processing time selected is inadequate for dehydration 5 Additives used in some commercial formalin fixative preparation...

Страница 119: ...completely detached from surrounding WAX when the tissue is observed the next day Tissue block very difficult to section 1 Processing time is inadequate for the tissue size selected resulting in inco...

Страница 120: ...ore critical to accommodate the larger sizes 2 Use of isopropanol as clearing agent for fatty tissues is inefficient for the removal of fats especially the larger blocks of breast 3 Reuse life of dehy...

Страница 121: ...th residual amounts of the previous solution remaining Indicated by continued bubbling originating from the tissue blocks in the final stage near the end of the WAX program Does not occur in smaller b...

Страница 122: ...he centre of the larger blocks 1 Tissue has been sufficiently dehydrated and cleared dry tissue however WAX impregnation is not complete Indicated by continued bubbling originating from the tissue blo...

Страница 123: ...here there are abundant red blood cells Present in tissues with hemorrhagic sites such as major blood vessels where there are abundant red blood cells and related breakdown deposits this especially in...

Страница 124: ...connective tissue parenchyma to hold fat cells in place connective tissue provides a framework to support fatty cells in place More likely to be seen in larger fatty tissue blocks and not in smaller f...

Страница 125: ...ol inefficient for fat clearing Use JFC as alternate clearing agent Reduce tissue block thickness Dehydration Clearing agent reuse life exceeded Replace Dehydration or Clearing reagent Collapse of blo...

Страница 126: ...ading procedure 3 Check that the reagent tanks hoses are not bent or occluded by anything Then press Clear to repeat the unloading procedure 4 Check that the hose connector is clean and properly inser...

Страница 127: ...d by the instrument see back label Voltage must be the same 10 of that indicated on this label 5 Call your customer service if the issue can t be fixed and press Help Desk to manage specimens while th...

Страница 128: ...estart the cycle with samples using step start procedure Select the correct step to start from in the following table NOT GOOD WETNESS A Follow the recovery laboratory internal protocols for air dried...

Страница 129: ...Running tap water for 30 minutes 4 Place in formol glycerol solution until tissues become soft and pliable with gentle pressure Tissues may remain in formol glycerol for up to 8 hours without adverse...

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