7)
Check the effect achieved when the collector lens focus knob is adjusted
and continue to make minor adjustments to the arc position and the mirror
position until the light is as even and centered in the FOV as possible.
8)
Remove the plastic slide and place a known good biological fluorescence
sample on the stage and again check the effect achieved when the collector lens
knob is adjusted and continue to make minor adjustments to the arc position and
the mirror position until the FOV displays an even an image as possible.
9) If a shadow is seen the FOV, you do not have the field iris centered AND the
field iris not open all-the-way.
2.6 Bulb Alignment – MT Models
After the bulb has burned in, the system is ready for arc lamp alignment.
There are many methods to accomplish arc lamp alignment if one were to go
look on the internet. The following is only one of many methods.
1)
If the arc lamp is not already burning, turn on the power supply and let the
arc lamp “settle” for at least 5 minutes.
2)
On the stage of the MT, place a piece of plastic on the stage that
fluoresces with any of the filter sets installed as shown below. One may have to
find the appropriate color of plastic that fluoresces with the filter sets you have or
buy a set of fluorescing slides from Chroma Technology Inc or
