6-8
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6.1.3 Cleaning and Replacing the Check Valves
This section provides the following check valve procedures:
•
Cleaning the check valves
•
Replacing an inlet check valve
•
Replacing an outlet check valve
Cleaning the Check Valves
Clean the check valves (see Figure 6-1) every six months, or more frequently if your application
warrants. It is a good idea to keep a spare set of clean check valves on hand. If you need to
remove the check valves for a full cleaning, you can install the spare check valves immediately
and clean the dirty check valves at a convenient time.
Required materials
•
If you use organic eluants – 1 L of a compatible cleaning eluant, such as a 1:1 mixture of
methanol and water with liquid detergent
•
If you use aqueous buffers – 1 L of a mixture of water and liquid detergent
On-line cleaning
To clean the check valves while they are installed in the system:
1.
Set the following operating parameter values (refer to Section 3.3, Reviewing and Editing
Operating Parameters:
•
Priming Flow Rate to 10.0 mL/min
•
Priming Pump Time to 15 min.
2.
Fill the pump eluant reservoir with at least 400 mL of cleaning eluant.
3.
Open the priming valve of the pump to be cleaned.
4.
Position a large beaker under the priming valve.
5.
Use the Prime Pumps function to pump the cleaning eluant through the pump and into the
beaker below the priming valve.
6.
Repeat steps 1 to 5 if additional cleaning is required.
7.
When you finish, close the priming valve.
8.
Restore the Priming Flow Rate and Priming Pump Time operating parameters to their
original values.
Full cleaning
If the on-line cleaning procedure is insufficient, take the check valves off the pump (as described
in the next two sections) and immerse the valves in an upright position for 10 minutes in
methanol. If a check valve does not work properly after cleaning, replace the valve.
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