4-5
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2.
Wait for the pump pressure to be stable (about 1/2 hour). Start the CHDF Software.
Follow the software manual instructions for data collection and particle size distribution
results.
3.
With the manual injector valve in the Load position, insert the prepared sample syringe in
the manual injector valve (Figure 4.5).
Note: The loop volume of the instrument is 20
µ
L. Load a minimum of 80
µ
L of sample to ensure
accurate results.
Figure 4.5 Loading Sample Into the Manual Injector Valve
Note: The manual injector valve must be in the Load position when you load the sample.
4.
Load the sample into the manual injector valve. Do not remove the syringe until you have
completed step 4.
5.
Rotate the handle of the manual injector valve to the Inject position to inject the sample
and start the selected method. The Analyze/Monitor Samples screen appears (Figure 4.5).
Remove the sample syringe.
6.
Rinse the injection valve with GR-500 eluant. Repeat the above steps to inject the marker
solution. The recommended marker injection time is 60 seconds.
7.
During the fractionation, the results will be displayed on the screen shown in Figure 4.4.
Also, relevant measurements such as the eluant temperature, pressure, flow rate, eluant pH,
eluant conductivity, and detection wavelength are displayed. Repeat the above steps for
new samples. Select Stop Pump or Home Screen to end your session.
8.
Review your results. If they are satisfactory, you are ready to analyze the next sample. If
the results are not satisfactory, refer to Chapter 5, Troubleshooting the CHDF 2000, to
locate the source of the problem.
Handle
Manual
injector
valve
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