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General Guidelines for Using the
LUNA™ Automated Cell Counter
In order to achieve the best results with the
LUNA™ Automated Cell Counter, users must follow the
instructions below carefully.
1. The instrument must be operated in compliance with the operating environment described on
page 7. In particular, the temperature and humidity conditions are important.
2.
Samples must be handled in an appropriate way, depending on user’s requirements.
3. Hold the LUNA
™ Cell Counting Slide by the edges to avoid touching its optical surface. Make
sure that no damage or contamination occurs on the optical surfaces of the slide.
4. After mixing the cell sample with Trypan Blue Stain, perform cell counting as soon as possible,
within 3 minutes for accurate cell viability measurement. If needed, count your sample at least 2
times (duplicate readings) and take an average.
5. Since the
LUNA™ Automated Cell Counter is calibrated before shipping, users generally do not
need to re-calibrate before use. However, if re-calibration is needed, please refer to Section 5.3
Calibrating the Counter.