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2.6 Counting Options
2.6.1 In the “Counting Options”, users can adjust Staining Option and Auto Exposure function.
<Staining Options>
2.6.2 In the
“Staining options” of “Counting Options”, users can choose one of the three staining
options as below.
Staining Options
Description
With Trypan Blue
(1:1)
This option can be used for normal bright field counting, when cell samples
are mixed with Trypan Blue dyes in a 1:1 ratio. This option can generate
cell viability data. For samples with Trypan Blue Stain, the dilution factor
must be set to value “2”. Please check.
Without Trypan Blue
When samples do not contain Trypan Blue dye, turn on this button and
follow the directions in the message boxes. These instructions set the
“Dilution Factor”. For samples with Trypan Blue dye, the dilution factor
must be set to value “2”. For samples without Trypan Blue dye, the dilution
f
actor must be set to value “1”.
Note
: The cell counting parameters of the LUNA
™ are optimized with the
use of Trypan Blue. Low contrast from no dye staining may sometimes
cause abnormal results.
