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The LI-3100C now uses the entire 25 cm measurement width for high resolution
(0.1 mm

2

) mode, so it is important to compensate for the above-mentioned edge

effect for the calibration procedure. While round discs like those previously used are
simple to manufacture with a precise area, this shape has the least amount of edge
(in this case circumference) compared to its area.

As a result, large changes could be made to the calibration adjustment with little
effect on the resulting readout. A large adjustment that makes the average reading
appear closer to the area of the round disc could now increase the errors in samples
with a high ratio of edge to area.

To address this issue, LI-COR is providing a new 10 cm

2

calibration plate with a

higher edge to area ratio. The user will find that the calibration adjustment is very
sensitive to small changes with this new calibration standard. This “edge-intensive”
standard is run through the area meter in a number of positions along the meas-
urement width, after which the round calibration discs are run through in a similar
fashion; the round discs should still produce results within the basic accuracy spe-
cifications. Thus, the user can view the response of the instrument to the different
extremes of sample geometry, and is assured of a better calibration. In addition, for
exacting measurements, the user can see which area of the measurement zone yields
the highest precision and accuracy.

Calibration procedure

1

Start the lamp and allow it to warm up for 5 minutes or more. Make sure that the camera position
and lens settings match the marks indicated for 0.1 mm

2

resolution (see

Changing measurement

resolution

on page 4-1). If transparent belt flaws, or debris on the belt is causing spurious counts

on the display, you may need to clean or replace the belt, as necessary.

2

Run the edge-intensive standard through the area meter at least 10 times, and average the read-
ings. Repeat over all of the areas of the sample width area. For example, use 5 zones from front to
back, and repeat to total 10 readings. If the error exceeds 2%, carefully turn the CAL screw a small
amount, clockwise to increase, or counterclockwise to decrease. Avoid changing the adjustment
too much. It is better to perform several trials, as normal sampling statistics will cause each trial to
vary slightly.

3

As a confirmation, perform the same 10 measurements with the 10 cm

2

round calibration disc. The

errors should remain within the specified limits. It is not necessary to turn the CAL screw until the
errors are zero; turning the screw more than a few degrees can actually increase errors in meas-
urement accuracy.

Section 3. Operation

3-3

Calibration procedure

Содержание LI-3100C

Страница 1: ...LI 3100C Area Meter Instruction Manual...

Страница 2: ......

Страница 3: ...da envsales licor com Regional Offices LI COR Biosciences GmbH Siemensstra e 25A 61352 Bad Homburg Germany Phone 49 0 6172 17 17 771 envsales gmbh licor com LI COR Biosciences UK Ltd St John s Innovat...

Страница 4: ...nting History Copyright 2004 2021 LI COR Inc All rights reserved Publication Number 984 07704 1st Printing June 2004 Updated September 2021 removed information about old operating systems minor reorde...

Страница 5: ...3 1 Belt tension and tracking adjustment 3 2 Calibration 3 2 Some history 3 2 Calibration procedure 3 3 Pressing roller adjustment 3 4 Section 4 Changing measurement resolution Changing from 1 0 mm to...

Страница 6: ...s 6 3 Section 7 Maintenance Changing the fluorescent tube 7 1 Changing the transparent belts 7 1 Alternative belt removal technique 7 3 Replacing the transparent belts 7 3 Cleaning the transparent bel...

Страница 7: ...d circuit board mounted on the instrument rear plate Figure 2 1 on page 2 2 Data are presented on the light emitting diode LED display Decimal location on the display is changed to suit the 1 0 or 0 1...

Страница 8: ...Section 1 General information 1 2 LI 3100C Area Meter Manual...

Страница 9: ...orescent tube Using the 5 32 4 mm hexagonal key provided remove the four retaining screws one at each end one at the center front edge on top and one at the center bottom edge at the rear Lift the hou...

Страница 10: ...push the sliding bear ing block The knurled knobs located on the pulley bearing blocks must be loosened to allow block movement Figure 2 1 Area Meter with rear housing removed A Main printed circuit...

Страница 11: ...on the next page 2 Hold the new tube vertically and gently tap it to cause any internal debris to collect at one end Internal debris can cause spurious counting 3 Slide the tube into the exposed hole...

Страница 12: ...Section 2 Preparations Figure 2 3 Area meter with fluorescent tube connector housing removed A fluorescent tube connector housing B fluorescent tube with exposed pins 2 4 LI 3100C Area Meter Manual...

Страница 13: ...oltage The instrument will not function on 100 VAC The voltage selector is changed by sliding the circuit board outward from its mount and repositioning it so that a different number is visible as app...

Страница 14: ...Section 2 Preparations 2 6 LI 3100C Area Meter Manual...

Страница 15: ...2 seconds If the fluorescent tube does not illuminate repeat the procedure l Press the Zero button to clear the display Figure 2 2 on page 2 3 Display response The display will rapidly accumulate num...

Страница 16: ...ly decrease the accuracy of finely detailed samples Some history Two calibration discs were included with LI 3100 Area Meters so that users could make minor adjustments of area readout using the CAL s...

Страница 17: ...remes of sample geometry and is assured of a better calibration In addition for exacting measurements the user can see which area of the measurement zone yields the highest precision and accuracy Cali...

Страница 18: ...n for use with the thinnest leaf types is shown in Figure 3 2 on the facing page To raise the pressing roller the lower belt tension must be released to prevent stretching To do this simply loosen the...

Страница 19: ...Figure 3 2 Press roller in position for use with thin leaves Section 3 Operation 3 5 Pressing roller adjustment...

Страница 20: ...Section 3 Operation 3 6 LI 3100C Area Meter Manual...

Страница 21: ...belts causes excessive spurious counts Changing from 1 0 mm to 0 1 mm resolution The procedures for the resolution change are outlined in the following steps 1 Disconnect the power 2 Remove the rear h...

Страница 22: ...red for 0 1 mm2 operation 6 Lightly tighten the set screws 7 Be sure that the lens cover has been removed and that no other obstruction is in the optical path 8 Replace the rear housing Calibration sh...

Страница 23: ...t screws on the outer camera pressure rail Figure 4 2 LI 3100C camera position configured for 1 0 mm2 operation 5 Align the yellow focus mark to the red lens index mark 6 Lightly tighten the set screw...

Страница 24: ...Section 4 Changing measurement resolution 4 4 LI 3100C Area Meter Manual...

Страница 25: ...o enter optional remarks for each logged area value The res ulting data file can be opened by most common text editor or spreadsheet pro grams l Supports serial and USB connections Installing the soft...

Страница 26: ...nection The RS 232 serial cable included has 9 pin connectors on both ends either end plugs into the serial connector on the back panel of the LI 3100C Use this cable to con nect to your computer s 9...

Страница 27: ...t of the area counter along with the selected resolution The PC area counter can be reset by the user this also resets calculated values for width and length Resetting the counter on the software does...

Страница 28: ...ced in the file header are optional The user may choose to be prompted for up to three remarks each time an area is logged to the file Columns for subtotal of area max imum width average width length...

Страница 29: ...pressed by checking the Zero values after Record check box Or values can be manually zeroed by pressing the Reset Counter button under the software area counter The Subtotal column represents an accu...

Страница 30: ...0C and the PC during operation a red asterisk will appear to the right of the area display window on the LI 3100C software This asterisk indicates that a data packet has been missed The next time data...

Страница 31: ...d into a spreadsheet Using the LI 3000A readout The LI 3100C serial port can be converted to connect to the readout console of the LI 3000A Portable Area Meter for those users who desire this function...

Страница 32: ...Section 5 Using the Windows interface software 5 8 LI 3100C Area Meter Manual...

Страница 33: ...2 5 Altern atively tip the instrument to about 45 by raising the front end to slide the debris to the end of the tube l Low line voltage Check it and provide proper voltage to the instrument l Belts...

Страница 34: ...l Portions of the sample protrude beyond the sensitive zone as defined by the sample guides Be sure that samples remain within the sensitive zone l Static electricity may be causing movement of dry sa...

Страница 35: ...e activated by connecting the two pins together momentarily Laboratory conditions Do not allow exposure to corrosive vapor Many laboratory procedures cause excess ive corrosive vapor concentrations as...

Страница 36: ...Section 6 Troubleshooting 6 4 LI 3100C Area Meter Manual...

Страница 37: ...Changing the transparent belts The following steps are used to remove the transparent belts An alternative tech nique is outlined in Alternative belt removal technique on page 7 3 1 Disconnect the po...

Страница 38: ...not loosen those screws 7 Remove the front plate slowly keeping it parallel to prevent binding on the shafts Be certain that all pulleys and idlers remain in place resting on the support brackets Thi...

Страница 39: ...te bearing Be sure that the peripheral end groove is toward the front and that it rests in the pul ley support bracket Replacing the transparent belts 1 Slide the lower belt onto the pulleys This requ...

Страница 40: ...s lens paper Cleaning the interior of the camera Cleaning within the camera housing is discouraged unless there is evidence of debris on the scanner spurious counts Removing the lens requires two reta...

Страница 41: ...brush Section 7 Maintenance 7 5 Cleaning the motor and belt drive system...

Страница 42: ...Section 7 Maintenance 7 6 LI 3100C Area Meter Manual...

Страница 43: ...connector located near the instrument front plate and under the belt drive pulleys This connector may remain mated if the front plate can rest near the instrument without straining the cord see Figure...

Страница 44: ...2 3 on page 2 4 A 7 64 278 cm hexagonal key is required for the socket set screw This key is not provided with the instrument Then remove the upper pulley 12 Replace the front plate connect the fluor...

Страница 45: ...one of measurement e g the center Samples with a high proportion of edges such as conifers roots wires etc will raise uncertainties to 5 and more Use only 0 1 mm2 resolution for these types of samples...

Страница 46: ...Appendix A Specifications Operating Temperature 15 to 55 C Storage Temperature 20 to 65 C Size 25 0 H 60 0 W 73 0 cm L 9 8 23 6 28 7 Weight 43 kg 95 lb A 2 LI 3100C Area Meter Manual...

Страница 47: ...d by act of God or other casualty 4 The purchaser whether it is a DISTRIBUTOR or direct customer of LI COR or a DISTRIBUTOR S cus tomer packs and ships or delivers the instrument to LI COR Inc at LI C...

Страница 48: ...apply whether included by this contract or by law are limited to the time period of this warranty which is a twelve month period commencing from the date the instrument is shipped to a user who is a...

Страница 49: ......

Страница 50: ...al Offices LI COR Biosciences GmbH Siemensstra e 25A 61352 Bad Homburg Germany Phone 49 0 6172 17 17 771 envsales gmbh licor com LI COR Biosciences UK Ltd St John s Innovation Centre Cowley Road Cambr...

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