31
8. Mounting troughs (‘Trufs’) onto the knife
For room temperature ultrathin sectioning disposable plastic troughs - called ‘Trufs’ - are
used to carry distilled water where the sections float after sectioning.
Fitting a Truf is simple if the Leica EM MP is used
(Fig.42)
.
Wax is melted in the bath of the Leica EM MP, glass knives are lightly warmed on the
hotplate with the knife edge overhanging the edge of the hotplate as shown in
Fig.43
.
The attachment surfaces of the Truf are coated with molten wax by putting it onto the wax
bath as shown in
Fig 43
.
The Truf is then brought into contact with the pre-warmed knife and slid up until it is levelled
with the knife edge as shown in
Fig.44
. The Truf is held in this position for a second or two
until the wax has set.
Additional wax is applied on the right side of the Truf using the spatula supplied with the
Leica EM MP for further protection against leaks
(Fig.45)
.
Содержание EM KMR2
Страница 2: ...2 ...
Страница 4: ...4 ...
Страница 8: ...8 Fig 4 Scoring angle Real knife angle Fig 5 Stress line ...
Страница 10: ...10 Fig 6 Fig 7 Fig 8 Side view Fig 8 ...
Страница 12: ...12 Fig 9 1 2 3 4 5 6 7 8 9 10 11 ...
Страница 14: ...14 Fig 10 Moveable stop at position 1 Fig 11 Fig 11a ...
Страница 16: ...16 Fig 12 Fig 13 Fig 14 Fig 15 Fig 16 Fig 17 Moveable stop at position 2 ...
Страница 18: ...18 Freshly broken side Freshly broken side Fig 21 Fig 22 Notch of the front stop Notch of the rear stop Fig 23 ...
Страница 20: ...20 Fig 24 Fig 25 Fig 26 Fig 27 ...
Страница 22: ...22 Fig 28 Fig 29 Fig 30 Fig 31 Right hand knife Left hand knife Fig 32 ...
Страница 26: ...26 Fig 34 Fig 35 Fig 36 Sliding surfaces ...
Страница 28: ...28 Fig 39 Fig 40 Fig 41 ...
Страница 30: ...30 Fig 42 Fig 43 Fig 44 Fig 45 Hotplate Wax bath ...
