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Version 2.1, Revision K

Cleaning and Maintenance

7.2.13 Cleaning the reagent vessels

1. Remove the reagent vessels individually from the load drawer by the handle. Ensure that the

handle is in the correct position

(

→

P. 37 – 4.6.3 Prepare the reagent vessel, fill it and insert it

into the load drawer)

to prevent spilling the reagent.

Dispose of filled reagents in accordance with the local laboratory specifications.

All reagent vessels can be cleaned in a dishwasher at a maximum temperature of 65 °C using

a standard, commercially available detergent for lab dishwashers. The handles can remain

attached to the various vessels when doing so.

Warning

•

Reagent vessels are to be pre-cleaned manually before cleaning in a dishwasher. Any staining

residue that may be present is to be removed to the greatest extent possible to prevent

discoloration of the remaining reagent vessels in the dishwasher.

• Never clean plastic reagent vessels at temperatures higher than 65 °C since this can deform

reagent vessels!

7.2.14 Rack and handle

The racks are to be checked regularly for mounting medium residue and other possible

contamination.

1. The colored handle must be removed from the rack for cleaning.

2. To remove small amounts of residual mountant, soak a lint-free cloth with xylene and clean the

racks.

3. For stubborn dirt, you can place the racks in a xylene bath for up to 1–2 hours to remove dried

out residual mountant.

4. The xylene can then be washed off with alcohol.

Completely rinse the racks with clean water and then dry them.

A dishwasher can also be used for the cleaning process. The maximum temperature of 65 °C must

be observed.

Note

The maximum exposure time in a xylene bath is 1–2 hours. Completely rinse the racks with water, then

dry them. If an external oven is used for drying, the temperature must not exceed 70 °C.

Warning

•

The racks and handles may not remain in the xylene for a long time frame (e.g. several hours or

overnight), because this can lead to deformation!

Содержание 14051454200

Страница 1: ...PECTRA CV Coverslipper Instructions for Use English Order No 14 0514 80101 Revision K Always keep this manual with the instrument Read carefully before working with the instrument Version 2 1 Revision...

Страница 2: ......

Страница 3: ...illustrations and other information regarding the contents or technical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determ...

Страница 4: ...ir connection 27 4 4 Installing the accessories 27 4 4 1 Fit the drawer inserts into the unload drawer 27 4 4 2 Inserting the waste tray 28 4 4 3 Filling and inserting the needle cleaning container 29...

Страница 5: ...starting the coverslipping operation 92 6 5 1 Procedure of the coverslipping operation 92 6 6 Starting the coverslipping operation 94 6 6 1 Monitoring the coverslipping operation 97 6 6 2 Coverslippin...

Страница 6: ...ng 131 8 1 Troubleshooting 131 8 2 Power failure scenario and instrument failure 136 8 3 Manual removal of a rack in the event of instrument malfunctions 138 8 3 1 Malfunction at the coverglass recept...

Страница 7: ...escription Software symbols that have to be pressed on the input screen are displayed as bold gray and underlined text Symbol Title of the symbol Keys and switches on the instrument Main switch Descri...

Страница 8: ...the requirements of both Canadian and U S Standards or with adjacent indicator US for US only or without either indicator for Canada only Symbol Title of the symbol In vitro diagnostic medical device...

Страница 9: ...Instrument surfaces or areas which become energized during operation are marked with this symbol Therefore direct contact is to be avoided Symbol Title of the symbol Caution danger of crushing Symbol...

Страница 10: ...that needs to be protected from moisture Symbol Title of the symbol Do not stack Description Stacking of the transport package is not allowed and no load should be placed on the transport package Sym...

Страница 11: ...ator has been triggered The Instructions for Use are accompanied by a bound RFID Registration supplemental sheet This supplemental sheet contains country specific information for the user about the me...

Страница 12: ...e with all inspection and maintenance instructions along with the observance of all notes in the Instructions for Use as well as the constant inspection of the media being used for storage life and qu...

Страница 13: ...st be notified Only original spare parts and approved original Leica accessories may be used Electromagnetic compatibility emitted interference and immunity to interference are applicable as are the r...

Страница 14: ...g distance during operation Warning The product uses a class 1 laser source Attention laser radiation Do not look into the beam This can cause injury to the retina of the eye Warning LASER RADIATION D...

Страница 15: ...s the case wait at least two hours before switching on the instrument The installation of the instrument at the area of use and a possible transport to a new location can only take place with the help...

Страница 16: ...ll ignition sources at least 2 meters away from the instrument Be absolutely certain to operate the instrument with the active carbon filter In addition we highly recommend connecting the instrument t...

Страница 17: ...d surfaces are not resistant to solvents and xylene substitutes The plastic reagent vessels can be cleaned in a dishwasher at a maximum temperature of 65 C Any standard cleaning agent for laboratory d...

Страница 18: ...4 0512 47084 1 Prime bottle assembly consisting of 14 0514 53931 1 Lab bottle 150 ml 14 0514 56202 1 Screw cap 14 0478 39993 1 Prime bottle insert 14 0514 57251 1 28x3 mm O ring 14 0253 39635 1 Cleani...

Страница 19: ...formance 30 m3 h Exhaust extraction Active carbon filter and exhaust hose for connecting with an external exhaust system Heat emission 1100 J s A weighted noise level measured at 1 m distance 70 dB A...

Страница 20: ...verglass cartridge capacity Exclusively Leica consumables with 300 coverglasses per magazine Coverglasses Exclusively Leica consumables Available size 50 mm x 24 mm thickness No 1 In accordance with I...

Страница 21: ...12 3 8 13 4 9 14 5 10 15 1 Instrument hood 9 Access to oven 2 Left coverslip line L1 10 Operating switch 3 Right coverslip line L2 11 Screen with user interface 4 Load drawer 12 Fuses 5 Unload drawer...

Страница 22: ...Instrument Components and Specifications 3 3 4 General overview rear view Fig 2 4 4 3 3 1 2 5 1 Network connection 4 Access to activated carbon filter 2 Power supply 5 Height adjustable instrument fee...

Страница 23: ...1 10 Parking location 2 Right coverslip line L2 11 Prime bottle 3 Pick Place module 12 Alignment pins 4 Waste tray 13 Red bar 5 Coverglass cartridge 14 Shifter and shifter tongue 6 Needle 15 Needle cl...

Страница 24: ...ature between the storage location and the installation site and if air humidity is high at the same time A waiting time of at least two hours must be observed each time before switching on Failure to...

Страница 25: ...of the instrument Fig 1 8 is in the OFF 0 position 1 Connect the power cable to the power input socket on the rear panel of the instrument Fig 4 1 2 Plug the power plug into a grounded power socket 3...

Страница 26: ...ser confirms the removal of the rack by pressing the Ok button Then the instrument restarts The internal battery has to be charged if the instrument has been disconnected from a power supply for 4 wee...

Страница 27: ...tive carbon filter must be used for connecting to an external exhaust system as well P 117 7 2 15 Changing the active carbon filter Compliance with this is mandatory The owner operator must verify com...

Страница 28: ...ent Setup 4 Fig 8 1 3 2 4 Finally lift the unload drawer and slide it back in the instrument 4 4 2 Inserting the waste tray Remove the waste tray Fig 9 1 from the packaging and insert it into the inst...

Страница 29: ...aning container P 111 7 2 9 Removing the complete unit of the needle cleaning container has been fixed in both coverslip lines during initial installation Do not leave the needle in the park position...

Страница 30: ...annot be successfully performed During the instrument setup or if no consumables mounting medium and coverglass have been added the modules are displayed in the display as empty Fig 22 1 Switch the Ma...

Страница 31: ...initialization a verification of all stations fill level scan is carried out automatically Furthermore the level of the needle cleaning containers is checked during operation approximately every 4 ho...

Страница 32: ...ning reagent vessels in the load drawer Always fill or drain reagent vessels and the needle cleaning container outside of the instrument in compliance with safety information to avoid or reduce the ri...

Страница 33: ...rtridge into the instrument This prevents a possible sticking of the coverglass due to humidity An RFID chip is integrated into the coverglass cartridge which ensures that the consumables management s...

Страница 34: ...s been reached or exceeded the mounting medium may no longer be used Expired mounting medium is to be disposed of in accordance with the applicable on site lab guidelines Opened mounting medium has a...

Страница 35: ...mounting medium is taken out of the instrument e g in the event of transport it can be closed up again using the black plastic cap and retained However while doing so the expiration date of the opened...

Страница 36: ...crease in force Therefore proceed carefully to avoid slipping from your hand 4 Repeat the process for bottle L2 5 Slide the bottle sledge Fig 16 1 back until you feel it click into place 6 Close the h...

Страница 37: ...hed to the reagent vessel If this is not the case fasten the handle according to Fig 17 Fig 17 1 2 Correct filling of reagent vessels Note The reagent vessel must be filled with xylene P 32 4 6 Refill...

Страница 38: ...labeled covers only in cases where the reagent vessels are empty e g after shutting down the instrument A sufficient fill level is ensured if the level of the reagents is between the maximum Fig 17 1...

Страница 39: ...ress the screen displays the following main window Fig 19 after switching on 1 5 2 3 4 6 7 8 9 10 11 Fig 19 1 Status bar 7 Process status display menu 2 Process status display 8 Module status menu 3 C...

Страница 40: ...racks in the instrument load drawer unload drawer oven coverslip line Corresponding function keys are grayed out and therefore deactivated However at all times it is possible to Refill consumables mou...

Страница 41: ...gain 5 If warnings and notes are displayed during operation this notification symbol appears Pressing this symbol allows the last 20 active messages to be viewed again 6 Local time 7 The Process symbo...

Страница 42: ...if the daily Quick Prime P 121 7 3 1 Quick Prime has not yet been carried out Note Each ongoing processing is illustrated by a rack handle symbol It is shown in the same color as the actual rack hand...

Страница 43: ...levels and notifies the user of the following details Mounting medium designation Fig 22 1 Remaining number of slides Fig 22 2 Coverglass length Fig 22 3 Remaining number of coverglass Fig 22 4 The pe...

Страница 44: ...ng the load drawers the instrument automatically detects any inserted or removed racks The racks in both drawers are shown on the screen with the respective rack handle color Fig 23 1 Fig 23 2 Availab...

Страница 45: ...t status of all racks in process Here the respective handle of the rack is displayed in representational form with the respective color This display shows the standard display The Module Status Fig 19...

Страница 46: ...play depends on the configured language 1 6 2 7 3 8 4 5 9 Fig 24 1 Title bar 2 Input field 3 Delete the most recently entered character 4 Confirmation 5 Move cursor to left or right 6 Space key 7 Spec...

Страница 47: ...ot included in the special character keyboard Fig 25 hold the corresponding normal button on the keyboard 2 Example Holding the standard a button brings up other selection options Fig 26 3 Select the...

Страница 48: ...se the completely configured instrument for all routine applications It is not possible for this user group to modify programs and settings Supervisor The supervisor has the same access options as the...

Страница 49: ...ed with the respective symbol in the status bar Fig 20 on the top right Note The password configured at the factory should be changed during the initial setup To change the supervisor password proceed...

Страница 50: ...e for technicians trained by Leica and certified for this instrument type 5 8 Basic settings The Settings Fig 29 1 menu is opened by pressing the gear symbol Fig 29 Basic instrument and software setti...

Страница 51: ...r or Leica service technician can add other languages if available by using Import P 61 5 8 7 Data management 5 8 2 Regional settings Required access level Standard user supervisor Basic format settin...

Страница 52: ...Standard user supervisor The current date Fig 31 1 and local time Fig 31 2 can be configured in this menu by rotating the individual rollers Pushing the Save Fig 31 4 button saves the settings If you...

Страница 53: ...s Error and signal sounds Required access level Standard user supervisor In this menu audible note and error signals can be selected the volume can be configured and functionality can be tested The cu...

Страница 54: ...ton The Test Fig 32 5 button can be used to listen to the respective sound The volume can be adjusted incrementally by turning the roller 0 to 9 Sound Type 2 Error Fig 32 2 Warning The user must stay...

Страница 55: ...onfigured in the menu for oven settings Fig 33 The current oven settings are displayed after calling up the menu Note When starting the instrument the oven is normally enabled and the Exclude Oven Ste...

Страница 56: ...s is indicated by the button Fig 33 2 being colored red white 3 Apply the shutdown by pressing the Save Fig 33 5 button 4 Observe both of the following information messages Fig 34 1 and Fig 34 2 and a...

Страница 57: ...ig 33 1 button 2 If the oven is enabled this is indicated by the button Fig 33 1 being colored red white 3 Apply the activation by pressing the Save Fig 33 5 button and note the subsequent information...

Страница 58: ...arning Excluding the oven step leads to less effective drying and requires greater care when removing slides from the rack Including the oven step in the processing 1 To enable the oven step at the en...

Страница 59: ...th a mounting medium which has already adapted to the ambient temperature The viscosity has a direct influence on the application quantity of the mounting medium on the slide If the application quanti...

Страница 60: ...ective coverslip line L1 or L2 4 Note the subsequent information message and acknowledge it with Ok Fig 38 4 5 Exit the menu by pressing the Back Fig 38 5 button 6 To discard the settings do not press...

Страница 61: ...he event logs log files can be exported in this menu A USB memory stick connected to the USB port on the front side of the instrument Fig 1 7 is required for all exports and imports 1 2 3 4 5 Fig 39 N...

Страница 62: ...moved too soon In this case the export process must be carried out again Service export Fig 39 2 The Service Export function is used to save a lpkg file on a connected USB memory stick Fig 1 7 The enc...

Страница 63: ...ce technician Note Lab specific settings are not deleted when updating the software for the HistoCore SPECTRA CV 5 8 8 Event view Required access level Standard user supervisor A separate log file is...

Страница 64: ...HistoCore SPECTRA CV You can scroll up and down in the list and the log file using the arrow buttons Fig 41 4 Pressing the left button allows you to scroll through the event viewer page by page Pressi...

Страница 65: ...3 4 4 5 6 7 Fig 41 1 5 Time stamp 4 Arrow buttons 2 Serial number 6 Event ID 3 Installed Software version 7 Message Color Gray Indicates an event or information Color Orange Indicates a warning messa...

Страница 66: ...res having the Supervisor user status and is only possible if there is no rack in the instrument and the instrument is idle The User status only allows one parameter set to be displayed 1 2 7 8 9 3 4...

Страница 67: ...arameter set is now saved and can be selected in the parameter set menu Fig 42 L L To discard the parameter set press the Cancel Fig 43 3 button 1 2 3 Fig 43 5 9 2 Assigning a parameter set to a rack...

Страница 68: ...that the same color is assigned to the suitable parameter set in HistoCore SPECTRA CV which was created and is startable 1 2 3 Fig 44 Note All available colors are displayed in Figure Fig 45 If an abb...

Страница 69: ...played in the parameter set list L L Cancel Fig 45 3 is used to close the dialog without applying the changes 2 3 1 Fig 45 Note The color white is a wildcard color When inserting a rack with a white h...

Страница 70: ...medium for the first time P 32 4 6 Refilling consumables Changing the mounting medium is described in P 78 6 3 1 Changing the mounting medium bottle 5 9 4 Properties of the coverglass Leica offers cov...

Страница 71: ...ecking the 0 value under the given conditions and requirements e g specimen size specimen type and specimen thickness temperature and humidity in the lab and adjusting it where appropriate 1 To adjust...

Страница 72: ...e mounting medium The user must monitor the levels of the reagent vessels to ensure they are sufficient If the instrument is to be in a non running state for a moderate period of time or if the HistoC...

Страница 73: ...e rack back out of the load drawer and to place it in the other reagent vessel in the load drawer The instrument detects if a rack with a rack handle that has an unassigned color is inserted in either...

Страница 74: ...lip lines The user has access to various cleaning options for the hose system through the Prime Clean submenu Fig 49 7 1 6 2 3 4 7 5 Fig 49 1 Mounting medium name 5 Remaining amount of mounting medium...

Страница 75: ...rglass cartridge 7 Right coverslip line L2 4 Pick Place module Warning The system does not monitor the fill level of the reagent vessels in the load drawer Fig 50 1 The user is responsible for monitor...

Страница 76: ...er completing the initialization phase the main menu Fig 19 appears on the screen and the user is informed Fig 51 that a Quick Prime is required for the coverslip line in use Acknowledge the messages...

Страница 77: ...tialization of the instrument the consumables mounting medium coverglass fill level of the needle cleaning container are checked automatically P 43 5 4 Consumables Management System CMS Fig 53 If a co...

Страница 78: ...accordance with the applicable on site lab guidelines Avoid a delay while changing the mounting medium bottle in order to prevent the cannula from becoming stuck Thus do not leave the cannula in the p...

Страница 79: ...rasp the bottle sledge on the handle and move it forwards Fig 56 1 3 Carefully withdraw the cannula Fig 56 2 L2 from the mounting medium bottle opening and place in the parking location Fig 56 3 4 Rem...

Страница 80: ...ticeably clicks into place Fig 58 7 In the course of this the white protective membrane of the bottle is punctured Note Before inserting the drain tube into the new mounting medium bottle check the ca...

Страница 81: ...work Warning The warning messages in P 32 4 6 Refilling consumables must be observed when handling solvents like xylene 1 Select the Module Status P 74 5 11 Module status menu in the main menu and pre...

Страница 82: ...automatically read when it is inserted and the hood is closed Changing the coverglass cartridge in the L2 coverslip line is described in the following The same procedures also apply for the coverslip...

Страница 83: ...2 3 Remove the coverglass cartridge Fig 61 3 that is present in the instrument from the slot for the cartridge Fig 61 4 Note If there are still coverglass present in the magazine up to 30 slip of rema...

Страница 84: ...ss cartridge into the slot for the coverglass cartridge Fig 62 3 1 1 3 2 Fig 62 7 Close the instrument hood 8 After closing the hood the user is questioned as to whether the remaining coverglass have...

Страница 85: ...mported and the Module Status is updated Fig 64 Warning Wear cut resistant safety gloves P 18 3 1 Standard delivery packing list when inserting additional coverglass The maximum filling level of the c...

Страница 86: ...te tray 1 Open the hood 2 Check the waste tray for broken coverglasses 3 If broken coverglass exist remove the waste tray Fig 65 1 and clean it 4 Then insert the waste tray back in the position provid...

Страница 87: ...he load drawer opens 3 If present remove the cover for the reagent vessel 4 Check that the fill level is sufficient Fig 67 and the reagent is free of dirt In the event of contamination the xylene is t...

Страница 88: ...at the end of the work day to prevent evaporation of the filled reagent 6 3 7 Unload drawer 1 Press the drawer button on the unload drawer Fig 50 2 2 The unload drawer opens 3 Remove any racks that ma...

Страница 89: ...TRA CV cannot recognize or process any racks for 5 slides or racks produced by other manufacturers The instrument software detects the manual insertion of racks for 5 slides in the load drawer of the...

Страница 90: ...ach time the program is used For this purpose a selection menu opens on the screen automatically after inserting the rack Proceed as follows to attach or replace the rack handles Detach the handle fro...

Страница 91: ...can become damaged in the subsequent processing The stickers and or labels used on the slides must be resistant to the solvents xylene used in the instrument Labels that are used must not reach into t...

Страница 92: ...function P 121 7 3 1 Quick Prime Filling of the needle cleaning container P 81 6 3 2 Monitoring and refilling of the needle cleaning container Check for sufficient filling and correct position of the...

Страница 93: ...de back to its original position in the rack 11 The elevator moves to the next slide which the shifter transports to the coverslipping position Note In order to prevent slides from drying out during p...

Страница 94: ...e for starting and being carried out Warning The rack can be inserted only using the load drawer Accidentally inserting a rack into the unload drawer can lead to a collision and thus to an instrument...

Страница 95: ...ss the load drawer button again to close it again 6 After closing the load drawer the instrument detects the RFID chip in the colored handle 7 The detected handle color is shown in the process status...

Страница 96: ...to their original position within the rack The instrument detects and indicates when a rack has been inserted in the wrong orientation and has to be corrected by the user The system detects if a rack...

Страница 97: ...ate time process icon user status and icons indicating messages and warnings that occurred The position of the rack is detected in the load and unload drawer using RFID Note The last 20 active message...

Страница 98: ...eived indicating that the unload drawer is completely occupied Fig 76 Failure to observe the message may lead to instrument faults and specimen impairment Finished coverslipped racks remain in the ove...

Страница 99: ...utomatically pauses any coverslipping operations in progress during which coverslipping for the most recently processed slide is completed Closing the hood allows the coverslipping operations to conti...

Страница 100: ...process is being canceled Fig 79 3 The border of the window shows the color assigned to the parameter set the mounting medium used the coverslip size used and the stored volume 4 Press the Abort Rack...

Страница 101: ...HistoCore SPECTRA CV The description on the correct generation of staining programs can be found in the Instructions for Use of the HistoCore SPECTRA ST Using reagents that are incompatible with each...

Страница 102: ...ng this time period and racks cannot be inserted into the HistoCore SPECTRA CV manually The brief inspection before the daily startup P 92 6 5 Brief inspection before starting the coverslipping operat...

Страница 103: ...connected HistoCore SPECTRA CV 6 Estimated remaining time hh mm 7 Time at end of process On the HistoCore SPECTRA ST status display a symbol indicates whether there is a connection to a HistoCore SPE...

Страница 104: ...en completed are automatically placed in the unload drawer of the HistoCore SPECTRA CV and have to be removed by the user there L L As soon as a rack is placed in the HistoCore SPECTRA ST that has to...

Страница 105: ...gulations and the disposal regulations of your company lab Shutting down the instrument for longer breaks in work and switch it off at the end of the workday P 76 6 2 Switching on and shutting down th...

Страница 106: ...them outside the instrument 2 Check the xylene in the reagent vessels correct the level or replace the xylene if necessary P 32 4 6 Refilling consumables 3 Check the inside of the load drawer for reag...

Страница 107: ...ning messages in P 32 4 6 Refilling consumables must be observed when handling solvents 1 Open the load and unload drawers and check the interior space behind the drawers for broken glass and residual...

Страница 108: ...the rear area inside the instrument Fig 85 Fig 85 5 Soak a lint free cloth with xylene and remove any mounting medium residue that may be present You can use a commercially available vacuum cleaner t...

Страница 109: ...annula that prevents contamination from getting inside the internal hose system This filter is replaced every two years by a Leica service technician as part of annual maintenance 7 2 7 Cleaning the n...

Страница 110: ...hnician may exchange a needle unit An exchange of only the needle is not possible 7 2 8 Filling and changing the needle cleaning container For handling the needle cleaning container see P 81 6 3 2 Mon...

Страница 111: ...6 3 2 Monitoring and refilling of the needle cleaning container 1 2 3 Fig 88 Warning There is a danger of cutting injuries when removing the complete unit Therefore proceed with the necessary level o...

Страница 112: ...he inserts do not fall out into the unload drawer 6 Carefully reach into the interior of the instrument through the opening of the unload drawer and feel around the complete unit for the needle cleani...

Страница 113: ...ing container cannot be removed after the xylene bath the recommended course of action is to immerse the complete unit with the container in xylene for a long period In the meantime insert the needle...

Страница 114: ...e Pick Place module from dirt 3 Control the coverglass sensor pin Fig 91 3 for glued on materials and mobility L L Dispose of glass shards splinters and dust in accordance with laboratory specificatio...

Страница 115: ...ack filled with empty slides coverslipped for a function check 2 1 1 3 Fig 92 7 2 12 Cleaning the waste tray Warning Wear cut resistant safety gloves P 18 3 1 Standard delivery packing list for emptyi...

Страница 116: ...shwasher Never clean plastic reagent vessels at temperatures higher than 65 C since this can deform reagent vessels 7 2 14 Rack and handle L L The racks are to be checked regularly for mounting medium...

Страница 117: ...ulations applicable in the country of use 1 The user can access the activated carbon filter Fig 93 1 from the front of the instrument by opening the upper cover Fig 93 2 2 The activated carbon filter...

Страница 118: ...priming and cleaning Note The user must flush the hose system during daily instrument setup and when inserting a new mounting medium bottle This ensures that the needle is not blocked and the interna...

Страница 119: ...119 HistoCore SPECTRA CV Cleaning and Maintenance 7 1 3 2 Fig 94 Fig 95 3 Observe the following information message Fig 96 Fig 96...

Страница 120: ...the needle unit Fig 97 1 in the prime bottle Fig 97 3 and acknowledge the information message Fig 96 with OK Warning Do not close the hood while the needle is inserted in the prime bottle in order to...

Страница 121: ...rinsing program This step is necessary to ensure the flow rate of the mounting medium through the needle system The respective coverslip line is ready to operate only after Quick Prime 1 After the ste...

Страница 122: ...8 3 rinsing program must be carried out after each time a mounting medium bottle is replaced P 78 6 3 1 Changing the mounting medium bottle for the respective coverslip line L1 or L2 During an Extende...

Страница 123: ...e mounting quality gets poor increased bubble formation between the slide and coverslip or if the HistoCore SPECTRA CV is to be prepared for transport or not in use for a prolonged time frame longer t...

Страница 124: ...it into the cleaning bottle 10 Then confirm the information message Fig 101 with OK Fig 101 The cleaning process begins Note The cleaning process lasts approx 45 minutes The information message Fig 10...

Страница 125: ...d priming P 122 7 3 2 Extended Prime Following this the user is prompted to remove the cleaning bottle from the instrument and to insert a new bottle with mounting medium After the insertion acknowled...

Страница 126: ...If the instrument is to be prepared for transport or storage press the No Fig 103 2 button 4 Take note of the next information message Fig 105 which contains instructions and acknowledge with the Ok...

Страница 127: ...strument has been disconnected from a power supply for 4 weeks For this purpose connect the instrument to the socket and switch on the Main switch Fig 1 8 The charging time is approx 2 hours 7 4 Recom...

Страница 128: ...pins Check for residual mountant and clean if necessary Fig 3 12 E Coverslip lines L1 and L2 Check for surface dried residual mountant and clean if necessary F Reagent vessels in the load drawer Repla...

Страница 129: ...le D Touchscreen Check for dirt and clean if necessary For this purpose commercial household cleaner can be used P 106 7 2 2 TFT touchscreen E Racks For small amounts of dirt Clean the racks with a li...

Страница 130: ...ebris toward the drawer outlet and then use a commercially available vacuum cleaner to carefully clean them up A Make sure the instrument is idle P 76 6 2 Switching on and shutting down the instrument...

Страница 131: ...le cleaning container Consumables cannot be scanned RFID chip is dirty Carefully remove any contamination from the RFID chip and re scan the consumables by closing the hood RFID chip is defective Try...

Страница 132: ...reagent vessel in the HistoCore SPECTRA CV load drawer and in the last steps of the preceding staining process in the HistoCore SPECTRA ST Dispose of non compatible reagent in accordance with the app...

Страница 133: ...tongue Check the shifter and shifter tongue also below the shifter tongue for contamination clean with a lint free cloth moistened in xylene if appropriate During application of the mounting medium th...

Страница 134: ...hnician to replace the needle unit Error message L1 L2 mounting medium not detected Bottle sledge Fig 16 1 not inserted until it clicks in place Slide the bottle sledge Fig 16 1 back until you feel it...

Страница 135: ...topped up the magazine but pressed Yes or removed too many coverglasses after the message of detecting too many coverglasses in the magazine Insert a new coverglass magazine Check rack for uncoversli...

Страница 136: ...arted up The user is notified of the charging process by a note on the screen The instrument is not ready for use again until after charging After the power supply has been restored the instrument aut...

Страница 137: ...s from the instrument the instrument must be shut down Following shutting down the user can for example easily move the transport arm in order to carry out the necessary steps for removing the racks C...

Страница 138: ...the red retaining lever upwards Fig 109 2 Pull out the drawer entirely and lower it into the rescue position Fig 109 3 4 Locate rack by looking through the opening of the unload drawer Fig 109 4 5 Ins...

Страница 139: ...139 HistoCore SPECTRA CV Malfunctions and Troubleshooting 8 Note Place the extracted rack outside of the instrument and secure the slide against drying out 1 2 3 4 5 6 7 8 9 10 Fig 109...

Страница 140: ...t first check whether a coverglass has been lost during transport to the slide or whether the Pick Place module was unable to take a coverglass from the coverglass cartridge 1 To do this start by open...

Страница 141: ...e coverglass cartridge as well If there is no longer enough coverglass present insert a new coverglass cartridge 8 If the steps listed above have been carried out press the OK button and close the hoo...

Страница 142: ...or the potentially necessary removal of a rack from the elevator Paying attention to the displayed messages is mandatory Always read the messages on the display first then perform the task s described...

Страница 143: ...king Ok 1 2 Fig 114 6 Take note of the following message and close the hood 7 The instrument now attempts to move the rack to the uppermost position 8 After a short period of time a message appears on...

Страница 144: ...this position Then carefully pull the canted slide forwards Fig 115 3 remove it from the instrument Fig 115 4 and coverslip it manually 1 2 3 4 Fig 115 10 Align the shifter Fig 116 1 with the red barr...

Страница 145: ...ollowing message Before confirming execute the tasks stated in the message 13 Hold the rack with one hand Fig 118 1 loosen the locking mechanism by pulling on the black knob Fig 118 2 at the elevator...

Страница 146: ...remove the needle from the parking location Fig 120 1 and insert it into the holder Fig 120 2 of the coverslip line Make sure that the needle is positioned correctly and confirm the message 2 1 Fig 1...

Страница 147: ...the rack from the lower area of the left elevator 1 Moving the unload drawer into removal position rescue position 2 Reach to the left elevator with your left hand through the unload drawer or through...

Страница 148: ...y upwards Fig 122 3 the magnet holds the oven door in place Fig 122 4 3 If the rack is not attached to the gripper Fig 122 5 the rack can be removed from the oven Fig 122 6 Warning It is possible that...

Страница 149: ...149 HistoCore SPECTRA CV Malfunctions and Troubleshooting 8 1 6 2 7 3 8 4 5 Fig 122...

Страница 150: ...position 2 Insert your hand carefully into the instrument and remove the rack from the gripper of the transport arm To do so grasp the handle and pull it forward along with the rack toward the load d...

Страница 151: ...ation back into the HistoCore SPECTRA ST manually Fig 125 2 and remove the rack Fig 125 3 from the holder Fig 125 4 1 2 3 4 Fig 125 4 Following this remove the rack from the instrument and store it sa...

Страница 152: ...damage to the fuse holders Beware of defective fuses Potential risk of injury due to broken glass Wear suitable protective clothing safety goggles cut resistant gloves P 18 3 1 Standard delivery pack...

Страница 153: ...Rack adapter for Stainer ST5010 ST5020 14 0475 58558 Reagent vessel assembly each consisting of 1 pc 14 0512 47086 Reagent vessel 14 0512 47081 Reagent vessel cover 14 0512 47085 Reagent vessel handle...

Страница 154: ...Consumables 9 Fig 127 Exhaust hose 1 set consisting of the following Exhaust hose of length 2 m Hose clamp Order No 14 0422 31974 Fig 128 Active carbon filter 1 set consisting of 2 pcs Order No 14 05...

Страница 155: ...SPECTRA CV Optional Accessories and Consumables 9 Fig 130 Needle cleaning container 1 kit consisting of 2 pcs Order No 14 0514 54195 Fig 131 Reagent vessel Assembly incl reagent vessel cover Order No...

Страница 156: ...pecimen slides 3 pcs per package Color Order No yellow 14 0512 52476 light blue 14 0512 52477 dark blue 14 0512 52478 pink 14 0512 52479 red 14 0512 52480 light green 14 0512 52481 black 14 0512 52482...

Страница 157: ...al Accessories and Consumables 9 Fig 135 Cleaning bottle Assembly Order No 14 0514 57248 Fig 136 Suction cup 1 pcs Order No 14 3000 00403 Fig 137 Storage rails for the unload drawer 1 set consisting o...

Страница 158: ...5 5x150 Leica brush T16A fuse Order No 14 0514 54189 Fig 140 Rack adapter for Stainer ST5010 ST5020 To be used to stain slides in SPECTRA racks in the ST5010 or ST5020 Order No 14 0475 58558 Consumab...

Страница 159: ...ica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Yearly Preventive Maintenance Leica recommends to conduct a yearly preventive m...

Страница 160: ...el Please refer to the Chapter P 105 7 Cleaning and Maintenance and the Chapter Decontamination Certificate P 161 12 Decontamination Certificate at the end of these Instructions for Use for notes on c...

Страница 161: ...product menu This template must be used to enter all required data If a product is returned a copy of the completed and signed decontamination certificate must either be enclosed or handed over to a s...

Страница 162: ...162 Version 2 1 Revision K Notes...

Страница 163: ......

Страница 164: ...LeicaBiosystems com Version 2 1 Revision K 07 2021 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 19 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems c...

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