Specimen holder scales
These scales allow easy identification of the specimen’s position
(co-ordinates), making it easy to return to a particular region of
interest after scanning the slide.
1. The horizontal coordinate can be read at position (1) on the
specimen holder (fig.18).
2. The vertical coordinate can be read at the index line (2).
Focusing Procedure
Working Distance (WD)
1. Rotate the coarse adjustment knob (1) clockwise so that the
objective (3) is as close as possible to the specimen (We
recommend starting with 10X). See figure 19.
2. While observing the specimen through the eyepieces, slowly
rotate the coarse adjustment knob (1) counterclockwise to lower
the stage.
3. When coarse focusing of the specimen is obtained (an image is
detected), rotate the fine adjustment knob (2) for fine focusing.
The WD refers to the distance between each objective and the
specimen, when precise focus of the specimen is obtained.
3
Adjusting the Focus
Objective
Magnification
4X
10X
40X
100X
WD (mm)
22
10.5
0.56
0.1
1
2
Cover glass
Slide glass
This is the glass plate placed on the specimen. For optimum
optical performance, the cover glass thickness, which is the
distance from its surface to the specimen surface, should be
0.17 mm.
This glass plate should ideally have a length of 76 mm, width of
2 mm ±1 mm and thickness
mm.
6
between 0.9 and 1.4
Cover glass
Slide glass
Fig. 17
Fig. 18
Fig. 19
Lx 100/200
1
2
3
WD
16
Issue 1.0
5030000-795 User Manual Lx-100/200 Printed on July,2013
