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H–8
IDEXX VetTest* Chemistry Analyzer Operator’s Manual
Calcium/Ca
2+
Calcium is an essential element which is involved in many body systems. These
include the skeleton, enzyme activation, muscle metabolism, blood coagulation, and
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Factors governing the total plasma or serum concentration are complex and include
interaction with other chemical moieties, proteins, and hormones.
Calcium, phosphorus, and albumin metabolism are interdependent.
Principal reasons for performing the test:
As an indicator of certain neoplasias, bone disease, parathyroid disease, and
eclampsia.
Most common abnormality indicated by the test:
Neoplasia
Special precautions in sample collection:
Centrifugation should take place quickly after the sample has been drawn. The sample
should not be exposed to the air for long periods. Glassware must be scrupulously
clean to avoid contamination by sources of calcium (e.g., detergents). Prolonged
contact with the clot may lead to lowered calcium values due to dilution by red cell
water.
Do not use tubes containing fluoride, oxalate, citrate, or EDTA as these agents will
cause significant negative interference. Total calcium results in patients receiving these
substances therapeutically may be falsely low.
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to reach room temperature before analysis.
Limitations of procedure:
Keeping the sample in an open container at room temperature may elevate the reported
test result. This effect is caused by the loss of carbon dioxide and change in pH of
the specimen. These changes are minimized by anaerobic handling procedures and
prompt analysis.
Complementary tests:
Calcium should be determined in conjunction with measurements of inorganic
phosphate, albumin, total protein, and glucose.
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