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C
Sample Collection and Preparation
The VetTest* Chemistry Analyzer is validated for animal plasma, serum or urine analysis
only, and should not be used for the analysis of other body fluids. The VetTest analyzer
is for veterinary use only.
Plasma and Serum Collection and Preparation
Minimize Stress
—Make sure the pet is calm when taking the blood sample. Use a
needle of appropriate size and gauge, and draw the blood smoothly to avoid inducing
unnecessary clotting or hemolysis. Tests most likely to show increases due to stress
include: aspartate aminotransferase (AST), creatine kinase (CK), glucose (GLU), lactate
dehydrogenase (LDH), inorganic phosphate (PHOS) and magnesium (Mg
2+
).
Fasting
—For best results, pets should fast for at least five hours before taking the
blood sample. It is often difficult, especially with cats, to be sure of when the animal
last ingested food. The effect of feeding on most test results is small compared with
changes seen in disease, unless a large meal has been consumed within five hours of
testing. The tests most likely to show important increases after ingestion of food are:
cholesterol (CHOL), glucose (GLU), inorganic phosphate (PHOS), triglycerides (TRIG)
and urea (UREA/BUN).
Plasma and Serum Sample Volumes
5IF7FU5FTUBOBMZ[FSSFRVJSFTBNJOJNVNPGÚ-PGTBNQMFUPSVOPOFUFTU5IFUBCMFT
below show sample requirements for 1–12 tests:
# Slides
Minimum Sample Volume
# Slides
Minimum Sample Volume
1
Ú-
Ú-
2
Ú-
8
Ú-
3
Ú-
Ú-
Ú-
10
Ú-
Ú-
11
Ú-
Ú-
12
Ú-
(FOFSBMMZÚ-PGXIPMFCMPPEZJFMETFOPVHITFSVNPSQMBTNBUPSVOTMJEFT
Note:
You should always consider the possibility that a dilution may be required to
follow up certain tests whose results fall out of range. See
Dilution Protocols
on page
&
for details.
General Blood Sample Collection Precautions
Use good technique to minimize stress to the animal and to avoid hemolysis.
t
Use the lowest gauge (largest) needle appropriate for the species.
t
Select the largest peripheral vein possible.
t
Always use new needles and syringes.
t
Use only the recommended collection devices.
t
Use an adequate volume of blood. Fill the collection devices to the manufacturer’s
t
recommended volumes.
Label the collection tube with the patient’s ID.
t
C–1
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