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nGauge AFM User Manual

 

16

 

 

and restarting the software again.   

Stage will not move 

Restart the software and make sure that the hardware connects properly 
by watching for the “AFM Ready” notification on the splash screen. 
The sample platform can only be lowered so far before a limit switch 
prevents further travel. Once the limit switch is triggered, you will not be 
able to retract any more, but you should be able to approach. However, if 
there is no functional AFM chip affixed to the stage, the platform cannot 
approach. Override controls exist in the 

Menu

 button on the top right of 

the screen. After clicking the 

Menu

 button, the 

Motor Controls

 can be 

used to approach without a functional AFM chip. 
If the stage still will not move, contact 

[email protected]

 for help. 

Auto approach will not 
run 

An AFM chip must be affixed and running at resonance in order to start 
the Auto Approach. If an AFM is already affixed, go to the Frequency 
Sweep tab and click 

Sweep

. A smooth curve with an obvious peak should 

be shown. If the maximum value of the peak is less than 2V, click on 

Advanced

 and increase the 

Amplitude

, then sweep again. If the top of the 

curve is cut off, decrease the amplitude and sweep again. If the frequency 
sweep plot data is flat and noisy, tighten the thumb nut on the AFM chip. 
If the problem persists, the AFM chip may be damaged. 

Frequency sweep flat 

A frequency sweep should return a smooth curve with an obvious peak. If 
instead the data is flat and noisy, try tightening the thumb nut on the AFM 
chip to make a better electrical connection, and sweep again. If the data is 
still flat and noisy, the AFM chip may be damaged. Click on the 

Menu

 on 

the top right of the screen and scroll down and click 

Read All

. If any of the 

values under Resistances is greater than 1000,

 

the AFM chip is damaged 

and must be replaced.  

Peak value of frequency 
sweep is lower than 1 or 
getting cut off above 3.3 

Click on 

show advanced options

 and adjust 

Cantilever Drive Amplitude 

(%)

. Increase the value if peak value is lower than 1; decrease if greater 

than 3.3. Generally, a drive amplitude of 20-35% is appropriate. 

Drift in Z when imaging 

If there are large line-to-line variations in height, the sample may not be 
secure. For instance, if imaging a lightweight sample (e.g. piece of paper), 
air drafts may cause significant drifts - fasten the sample down with a 
heavy object. 
If the approach completes early and the image does not contain 
topography information, it is possible that the surface is charged. One way 
to verify this is to perform another frequency sweep. If a complete curve is 
obtained, with the peak at a new frequency, the sample may need to be 
discharged before imaging. 

Noisy image  

If the image results are excessively noisy, this may be caused by vibrations 
or air drafts. Try moving the stage to a different location, placing the stage 
on some soft foam or bubble wrap, or place a box over the stage to 
eliminate drafts. 

Data "plateaus"  

The Z actuator is split into Coarse Z and Fine Z controls. The Fine Z has a 
smaller range, and it’s the control that is used to track sample topology 
while scanning. If a sample has tall features that extend beyond the range 
of the Fine Z control, the image will get clipped. To increase or decrease 
the Fine Z scan range, click on the 

Menu

, then under PGAs, adjust the Fine 

Z. For maximum imaging resolution, the smallest Fine Z range should be 

Содержание nGauge AFM

Страница 1: ...User Manual Version 1 1 Atomic Force Microscope...

Страница 2: ...3 1 Where to set it up 6 3 2 How to set it up 7 3 3 Installing the Software 9 4 0 Basic Operation 10 4 1 Software Overview 10 4 2 Preparation and Approach 11 4 3 Imaging 12 4 4 Analyzing Data 14 5 0...

Страница 3: ...ollowing warnings and precautions should be observed to ensure the safety of the user and the instrument Use only the power supply provided The driving electronics are designed to accept 7 5Vdc and an...

Страница 4: ...ng DOWN in the storage box Use tweezers to pick them up The AFM chip can be placed on a flat surface with the tip facing up The AFM chip can be picked up by hand as long as you don t touch the tip Thi...

Страница 5: ...of the cantilever onto a 4 quadrant photodetector that measures any small displacements of the cantilever A piezoelectric Z scanner controls the height of the probe while a piezoelectric XY stage ras...

Страница 6: ...mage due to surface charging 3 0 Setup 3 1 Where to set it up The nGauge AFM is small simple and portable and can be set up just about anywhere However for best results it is recommended that you adhe...

Страница 7: ...ng it up near sources of electrical noise such as server rooms or rooms operating high power equipment 3 2 How to set it up Setup of the nGauge AFM is very simple 1 Remove the components from the carr...

Страница 8: ...nGauge AFM User Manual 8 3 Connect the USB cable to a computer and to the USB connector in the stage 4 Completely unscrew and remove the thumb nut from the stage...

Страница 9: ...between the tip and platform 7 Replace the thumb nut and firmly tighten it 3 3 Installing the Software The software required to operate the AFM can be found on the USB stick provided or downloaded fro...

Страница 10: ...ftware Overview Launch the software by double clicking on the nGauge program on your desktop or searching for nGauge in the start menu If the AFM is powered up and connected to the computer with the U...

Страница 11: ...the platform is low enough Position the sample so that the area to be scanned is directly under the tip Ensure the sample is stable on the sample platform and that it won t tilt roll flutter drift or...

Страница 12: ...ls above each point of the scan and ultimately determines the scan speed A smaller number in this field increases the scan speed but if the scan is too fast the resulting image may be blurry or distor...

Страница 13: ...s more force A lower setpoint can often give better results but is also more likely to damage the tip or sample and when the setpoint gets too low the imaging performance may suffer A PI controller is...

Страница 14: ...want to extract some quantitative data about the sample like the height or width of a specific feature or the RMS roughness of the surface These measurements are best acquired using Gwyddion a powerf...

Страница 15: ...ay a 3D view of the image You can rotate or scale this 3D image change the colors and save it Once you are satisfied with the post processed image you can save it as JPG PNG etc using the Save As opti...

Страница 16: ...still flat and noisy the AFM chip may be damaged Click on the Menu on the top right of the screen and scroll down and click Read All If any of the values under Resistances is greater than 1000 the AFM...

Страница 17: ...The main controls that are visible in each screen should be sufficient for normal imaging conditions However each tab in the navigation contains advanced controls that can extend the functionality or...

Страница 18: ...e line is being scanned repeatedly Leveling Direction To correct for the 15 degree tilt of the AFM chip relative to the sample a hardware leveling feature is applied Leveling Direction switches betwee...

Страница 19: ...of the Coarse and Fine Z the amplitude of the AC driving signal on the Z actuator and the amount of leveling that is added to each scan PGA values can range from 0 140 ADCs Analog to Digital Converte...

Страница 20: ...Port release the USB connection Choose Firmware currently Windows only Opens a window that allows you to locate the firmware update Firmware update files are binary bin files and can be downloaded fro...

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