Hygiena BAX System X5, Руководство пользователя

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Chapter III. The BAX® System X5 PCR Assays
BAX® System X5 User Guide 53
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When transferring samples, begin and complete one column at a time as shown in the BAX® System Ready
Reference. Cap tubes after finishing a single row of samples to avoid cross-contamination.
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Do not shake or mix enrichments before transferring samples to cluster tubes. If enrichments have been
agitated, let sit for at least 10 minutes before transferring aliquots to cluster tubes.
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For sample enrichments with particulates, pipette samples from the upper layer of the sample, not the
bottom of the enrichment. Double-check the pipette to make sure it has retrieved the sample.
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If sample enrichments are slimy, insert only the tip of the pipette into the enrichment. Wipe off any excess
material by scraping the tip against the side of the sample container. In severe cases, you can use a clean
wipe or tissue (fresh for each sample), but care must be taken to avoid cross-contamination.
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For sample enrichments with an oil/fat layer, pipette samples from the aqueous layer below the oil/fat layer.
Wipe off any coating of oil/fat from the tip by scraping the tip against the side of the sample container or by
using a new clean wipe or tissue for each sample. If necessary, you can transfer an aliquot from the
enrichment to a secondary tube to facilitate a clean transfer to lysis tubes.
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When transferring samples from cluster tubes, draw sample from the middle of the tube so that only the
liquid is drawn into the pipette. Avoid drawing sample from the bottom of the tube.
Tips for PCR Tablet Hydration
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Do not remove the PCR cooling block from the refrigerator until it is needed. Finish using the cooling block
within 30 minutes of removing it from the refrigerator.
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Remove only the required number of PCR tubes from the Mylar bag. Re-seal the bag tightly and
immediately refrigerate any unused PCR tubes.
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When hydrating tablets, only remove the caps from one strip of tubes at a time to avoid cross-contamination.
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Visually check that each PCR tube contains a white, complete tablet before adding sample lysate. If tablets
have shrunken or appear pink, discard tubes and replace with new ones before proceeding.
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PCR tablets must be hydrated and re-sealed within 10 minutes after loosening the domed caps from the
PCR tubes.
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Keep samples chilled at 2-8°C until immediately before loading them into the instrument.