Hygiena BAX System X5, Руководство пользователя

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Chapter III. The BAX® System X5 PCR Assays
30 BAX® System X5 User Guide
Positive E. coli O157:H7 MP Melting Curve Profiles
Samples that are positive for E. coli O157:H7 will display two target peaks. These target peaks usually appear
between 81-90°C.
The graph also displays one INPC, or control peak, which usually appears between 79-82°C. If a sample displays a
strong positive result, the INPC may be very small in comparison to the target peaks.
Examples of Typical Melt Curves for Positive E. coli O157:H7 Samples
Negative E. coli O157:H7 MP Melting Curve Profiles
Samples that are negative for E. coli O157:H7 will display either one target peak on the melt curve (usually between
81-90°C), or no target peaks.
The graph also displays one INPC, or control peak, which usually appears between 79-82°C. If a large target peak is
also present, the INPC may be very small in comparison to the target peak.
Examples of Typical Melt Curves for Negative E. coli O157:H7 Samples
7. Confirm Positive Results
Method approved by AOAC
Positive results can be confirmed according to the AOAC confirmation method by following the reference culture
method appropriate for the sample type. To confirm results with the FDA-BAM method, see the protocol described in
the Bacteriological Analytical Manual. To confirm results with the USDA-FSIS method, see the protocol described in
the Microbiology Laboratory Guidebook.