Chapter III. The BAX® System X5 PCR Assays
36
BAX® System X5 User Guide
Negative Genus Listeria Melting Curve Profile
Samples that are negative for
Listeria
species will display no target peaks. The graph displays only the INPC, or
control peak, which usually appears between 76-81°C.
Example of Typical Melt Curve for Negative Genus Listeria Sample
7. Confirm Positive Results
Method approved by AOAC
Positive results can be confirmed according to the AOAC confirmation method by following the reference culture
method appropriate for the sample type. To confirm results with the FDA-BAM method, see the protocol described in
the Bacteriological Analytical Manual. To confirm results with the USDA-FSIS method, see the protocol described in
the Microbiology Laboratory Guidebook.
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