HP D300 Digital Dispenser
44
○
To start from a new Fluids List to design a new plate configuration, select
Clear all data
and start from scratch (no fluids, empty well plate)
.
Figure 5-17
Plate Layout Wizard: Initialization dialog box
b.
If you selected the first option, the next two questions involving assay volume and the
DMSO limit will be unavailable because the previous values are used. If you selected the
second or third option, type the assay volume and DMSO limit for the new plate design into
the boxes and press the ENTER key on the keyboard.
c.
The next question involving the number of fluids you are using also changes depending on
which option was selected above. Read the question carefully, type an answer into the box,
and press the ENTER key.
d.
Click in the first column of the table to edit the names of the additional fluids, if desired, and
then type the source plate/vial, source well, and concentration information into the following
columns.
e.
Select the
NoDispense
check box if the fluid should be a no dispense fluid (fluids are
which are non-HP dispensed fluids). There needs to be at least one fluid with
NoDispense
checked in order to reserve rows or columns for controls which are not dispensed by the
HP dispenser.
f.
To apply a concentration other than the default, type the appropriate value in the box at the
bottom of the Initialization dialog box and click the
Use this concentration for all fluids
button to apply the new value to all of the new fluids.
g.
Click
Continue
to move to the next step. The Plate Layout Wizard: Titrations and
Positioning dialog box appears. See
IMPORTANT:
Once titrations and positioning are initiated, changes cannot be made to the
fluids table because changes could disrupt the design. To make changes to the fluids table,
click
Cancel
, then make changes to the fluids table before launching the Plate Layout
Wizard again.
Select one of these
three options
Answer these
questions unless
you chose to leave
all existing data
Change concentration
here if desired
Type fluid
information
in this area
if desired
Select at least one
NoDispense to
allow placement of
controls
Содержание Compaq d300 Series
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