© 2017 by Hettich AG
page 25
CW II-OM-rev1.0
Hettich AG
Laborapparate
phone:
+41 44 786 80 20
Seestrasse 204a
e-mail:
CH-8806 Baech
internet:
www.hettich.ch
9.4.5
SPIN process
Sedimentation.
The number of revolutions per minute is adjustable
from 0RPM to 3500RPM. The time-duration is
adjustable from one second to two hours (0:00:01 to
2:00:00). The default-value is 3500RPM and
30seconds (0:00:30)
The erythrocytes are sedimented by a selectable
rotation speed. The time only begins to count after
the set
rotation speed is reached. After the time has
elapsed, a quick braking follows to prevent a
resuspension of the
pellet.
It is not possible to work with an endless spin process.
If it is necessary to work with a longer single spin process, it is possible to add a loop-process for the
desired time-duration
9.4.6
DECANT process
Decanting.
The number of revolutions per minute is adjustable
from 0RPM to 2500RPM. The excess is decanted at
the selectable rotation speed. During decanting, the
rotor turns in the opposite direction of the normal
rotation so that the solution will be decanted.
The default-value is 370RPM.
The correct speed must be validated with the used tubes for the best possible results because the different
inner surfaces of the tubes, e.g. glass-tubes or plastic-tubes, there will get also different results when the
speed is optimized for 10mm-tubes but the used tubes are 12mm-tubes.
If the DECANT speed is too high then it is possible that the washed cells will also be decanted!
If the DECANT speed is too low then it is possible that too much solution will stay into the tubes and during
the next FILL process the tubes will overfilled!
9.4.7
AGIT process
Agitating, shaking.
The numbers of shakings are adjustable from 0x to
500x. The default-value is 15x. The pellet is
loosened again for the subsequent wash cycle by a
fast, brief movement of the rotor and the tube-
holders.
