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© 2017 by Hettich AG
page 24
CW II-OM-rev1.1
Hettich AG
Laborapparate
phone:
+41 44 786 80 20
Seestrasse 204a
e-mail:
CH-8806 Baech
internet:
www.hettich.ch
9.4
Process descriptions
9.4.1
Principle
A program can have at maximum 20 different proceses, but only with 1 LOOP process. It doesn’t matther with which
process a program starts except with a LOOP process or a CHECK process and it could also have only one process.
9.4.2
FILL 1 process
Filling in the physiological saline solution.
The tubes are filled with physiological saline solution
at a rotation speed of 1100RPM. The number of
revolutions per minute is adjustable from 0RPM to
2500RPM. The fill-volume is adjustable from 0,1ml to
10ml per tube. The default-value is 900RPM and
3.0ml. The physiological saline solution is injected
directly into the tubes, in order to receive a good
resuspension of the cells.
The best results are with a speed of 900RPM for both kind of rotor-types.
The device will calculate the complete volume itself for the preseted rotor
9.4.3
FILL 2 process
Filling in the secondary solution.
The tubes are filled with solution at a rotation speed
of 1100RPM. The number of revolutions per minute
is adjustable from 0RPM to 2500RPM. The fill-
volume is adjustable from 0,1ml to 10ml per tube.
The default-value is 900RPM and 3.0ml.
Only selectable if your device have installed the optional second pump (part-number
900030, 900230,
1008-02 and 1008-04)
9.4.4
DOWN process
Down.
The number of revolutions per minute is adjustable
from 0RPM to 3500RPM. The time-duration is
adjustable from 0s to 20s. The default-value is
2000RPM and 5s. Centrifugation run to centrifuge
the remaining droplets on the inner-side of the tubes
to the bottom of the tube.