OPERATION OF THE JUNIOR-PAM
CHAPTER 5
measured during an post-pulse interval with only the far red il-
lumination switched on (Fo’-Mode, Box (7)).
5.1.5
Box (5) - Online Data
Online data in Box (5) represent digital values of current measurements
of the <Online> data introduced above (see <Val.> sidebar). Checking
the PAR* will add the PAR measured by an external quantum sensor to
the currently selected actinic PAR of the JUNIOR-PAM light intensity
as defined in the internal PAR list. Therefore, in the absence of external
light measurement, the PAR* in Box (5) corresponds to the actinic light
of the JUNIOR-PAM. In the case of the JUNIOR-PAM, the UBat indi-
cates the voltage supplied by the USB socket.
5.1.6
Box (6) - SAT-Pulse Trigger
In Box (6), both, the <Fo, Fm> and the <SAT> button, manually re-
leases a saturating light flash of interval and intensity defined in <Set-
tings>. Clicking the <Fo, Fm> determines the fluorescence yields <Fo>
and <Fm>. By definition, the <Fo> and <Fm> levels are properties of
dark-acclimated photosynthesis. With light-exposed samples, saturating
pulse analyses is initiated by the <SAT> button to determine F’, Fo’ and
Fm’. In total, WinControl-3 derives 9 different fluorescence quotients
from the 5 types of fluorescence yield (see Box (3) and Box (4)).
Box (6) displays the current value of Y(II)=(Fm’-F’)/Fm’ together with
the corresponding fluorescence trace during saturating pulse analysis. In
the graph, dashed lines indicate the levels of Fm’ and F’ used for Y(II)
calculation. The graph is viewed enlarged after clicking the <Graph>
tab.
5.1.7
Box (7) - Experimental Parameters and Procedure
Box (7) includes 3 groups of commands which determine different as-
pects of experimental procedures: the <Status>, <Basics>, and <Pro-
gram> field: tabs allow switching between the latter two fields.
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