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Unit Calibration
– Calibrating the Unit
If the system is not detecting colonies it will require calibration. The Wizard detects
colonies based on the contrast between the background color and the colonies. It
then determines if the colony is to be counted by checking it against a color and size
profile. The color is made up of Blue, Green and Red values. There are two methods
to calibrate the unit effectively.
1. Quick Sensitivity Adjustment
– If a unit is not detecting colonies, the Sensitivity
can be quickly adjusted in the EDIT screen from the Sensitivity Button.
However, it is
recommended to perform a complete calibration as listed in the next steps below.
This method is designed to be a quick work around for “on the fly” sensitivity
adjustments which are normally accomplished with a complete calibration. This
method will not change how a colony is categorized, it will only change if it is
recognized so a more in-depth method is required if the colony is not categorized
correctly. Press to proceed with the adjustment.
2. Configuration Menu Method
– The Configuration Menu Method breaks the calibra-
tion into two parts. This is the recommended method for configuring the unit for
quality control prior to use.
a.
Sensitivity Screen
– Determines what is a colony or detected object based on
contrast sensitivity and size. A boost function is added for enhanced detection of
pinpoint colonies and may be calibrated separately in the same screen.
b.
Colony Type Screen
– The Colony Type looks at all detected objects and deter-
mines if the object is the colony of interest based on color and size. For modes that
detect multiple types of organisms (YM and EC), multiple Colony Type Screens are
used. A boost function is added for enhanced detection of pinpoint colonies and
may be calibrated separately in the same screen(s).
Summary
– If a colony is not detected, the Sensitivity requires adjustment. If a colony
is detected but it is classified incorrectly then the Colony Type Screen will be used to
calibrate the unit. Both adjustments are required to complete a full calibration for
quality control. An added boost function for enhanced sensitivity may be calibrated
during normal calibrations.
Содержание The Wizard CompactDry Reader
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