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qCMOS Camera C15550-20UP Instruction manual_Ver.1.0

(10) Camera readout modes

The camera has three kinds of readout mode, Area readout mode, Lightsheet readout mode and

Photon number resolving mode. The camera also has two scan speed (Standard scan and Ultra

quiet scan), and two readout direction in Lightsheet readout mode.

(Camera readout modes)

(Scan speed)

(Readout direction)

Area readout mode

Standard scan

Ultra quiet scan

Lightsheet readout mode

Standard scan

Top to Bottom

Bottom to Top

Photon number resolving mode

Ultra quiet scan

(11) Camera operation modes

The camera has three operation modes: 1) Free running mode, in which the exposure and readout

timing are controlled by the internal microprocessor, 2) External trigger mode, in which the

exposure and readout timing are decided by an external trigger, and 3) Start trigger mode is used

to start operating the camera by a trigger input for a continuous imaging.

(Camera readout modes)

(Camera operation modes)

(External trigger modes)

Area readout mode /

Photon number resolving mode

Free running mode

External trigger mode

Edge trigger mode

Start trigger mode

Global reset

Edge trigger mode

Level trigger mode

Global reset

Level trigger mode

Synchronous readout

trigger mode

Light sheet readout mode

Free running mode

External trigger mode

Edge trigger mode

Start trigger mode

Содержание C15440-20UP

Страница 1: ...id personal injury and damage to property when using this camera The manual describes the correct handling method of the camera and provides instructions that should be followed to avoid accidents Rea...

Страница 2: ...Blank page...

Страница 3: ...and even death Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property This symbol indicates a cautionary item that should be f...

Страница 4: ...can lead to electric shock Do not attempt to dismantle or modify the camera Doing so can also lead to damage and even injury as some internal components become very hot or high voltage Do not touch p...

Страница 5: ...mount of the camera Doing so can scratch the protective glass Some wide angle lenses in particular can have a thread of 7 mm or more Shipping precautions When transporting the camera by truck ship air...

Страница 6: ...les These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate rad...

Страница 7: ...water other than soft water as recommended for example antifreeze etc refer to description of cooling water which is written in 12 MAINTENANCE or contact a Hamamatsu subsidiary or your local distribu...

Страница 8: ...15550 20UP Instruction manual CD ROM 1 QC sheet 1 Option Cooling water hose 2 hoses A10788 04 SMA BNC cable A12106 05 SMA SMA cable A12107 05 CoaXPress interface board M9982 30 CoaXPress interface cab...

Страница 9: ...e to a strong source of magnetism or radio waves Places where there are vibrations Places where the camera may come into contact with corrosive gases such as chlorine or fluorine Places where there is...

Страница 10: ...24 9 1 OPERATING PRECAUTIONS 24 9 2 PREPARATING FOR IMAGING 25 9 3 IMAGING 25 9 4 END OF IMAGING 25 9 5 STARTUP DCAM CONFIGURATOR 26 9 6 UPDATE PROCEDURE OF COAXPRESS INTERFACE BOARD 28 10 DESCRIPTIO...

Страница 11: ...3 TROUBLESHOOTING 63 13 1 IMAGE IS NOT TRANSFERRED 63 13 2 ALTHOUGH IMAGES ARE TRANSFFERED 63 14 SPECIFICATIONS 64 14 1 CAMERA SPECIFICATIONS 64 14 2 CONDENSATION 67 14 3 SPECTRAL RESPONSE CHARACTERIS...

Страница 12: ...logies and realizes extremely low readout noise 0 27 electrons r m s 2 Photon number resolving capability Photon number resolving is realized within each pixel enabling superior low light performance...

Страница 13: ...rection feature to further improve image quality The correction can be performed in real time without sacrificing any of the readout speed 9 Interface This camera has both CoaXPress interface and USB...

Страница 14: ...he camera has three operation modes 1 Free running mode in which the exposure and readout timing are controlled by the internal microprocessor 2 External trigger mode in which the exposure and readout...

Страница 15: ...tilation openings Lens mount C15550 20UP can be attached to C mount lens or an optics system Note The depth of the C mount is 7 mm Screwing in the C mount too deeply might scratch the glass surface WA...

Страница 16: ...MING 1 2 3 This is used when peripheral device s require synchronization with the camera Output is 3 3 V LVCMOS level Output impedance is 33 Note Determine termination according to cable length and so...

Страница 17: ...taneously Air inlet This is the inlet for the heat ventilation If the camera is being operated in an enclosed environment ensure to keep clearance at least 10 cm from both intake and exhaust vents whe...

Страница 18: ...ON Refer to the figure when connecting the various cables 1 CoaXPress interface Figure 7 1 2 USB interface Figure 7 2 CoaXPress interface board to connector 1 4 Circuit water cooler a b c Camera Rear...

Страница 19: ...interface is changed from CoaXPress to USB and vice versa the application software must be closed and the camera must be turned off Do not connect CoaXPress and USB interface simultaneously Note Hamam...

Страница 20: ...d for example antifreeze etc refer to description of cooling water which is written in 12 MAINTENANCE or contact a Hamamatsu subsidiary or your local distributor 3 Recommendation temperature Hamamatsu...

Страница 21: ...igure 8 1 If the hose size on circulating water cooler is the same as blue hose remove gray hose from the joint part The gray hose can be removed when blue hose is pulled with pushing the button of th...

Страница 22: ...on the microscope If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope 4 Connect t...

Страница 23: ...water or water drop inside the hose and the camera by air Blow air from one side of hose Prepare water absorption sheet such as Waste Towel or so and catch pan on another side of hose in order to avo...

Страница 24: ...ted before start operating the camera in water cooling A fan inside the camera does not rotate See 8 WATER COOLING for instruction of water cooling Do not switch to water cooling method when water coo...

Страница 25: ...rating properly and air is circulating When cooling method of the camera is set by water cooling method the fan does not start rotating 9 2 2 WHEN USING WATER COOLING 1 Connect the equipment as shown...

Страница 26: ...startup DCAM Configurator 1 Open Setup exe in the DCAM API software s folder If the DCAM API software is not installed on your computer insert the media of DCAM API software in the slot of your comput...

Страница 27: ...f the camera s power supply is turned off the state of setting is kept The state of setting can confirm according to Hardware icon on DCAM Configurator window After the startup operate DCAM Configurat...

Страница 28: ...ion of the interface board the following message prompting update of the interface board may be displayed When this message is displayed you need to update the firmware of the interface board to use t...

Страница 29: ...scientific CMOS image sensor used in this camera has an on chip CDS correlated double sampling circuit which plays an important role in achieving low noise In addition column amplifiers and A Ds are...

Страница 30: ...g in white spots Currently this phenomenon is not preventable If CMOS image sensor is at a fixed temperature recurrence of the white spot increases proportionally with the exposure time so this can be...

Страница 31: ...om camera individually for all pixels 2 Binning readout 2 2 4 4 readout With this camera 2 2 binning readout and 4 4 binning are available by adding the signal of adjacent pixels in the digital domain...

Страница 32: ...SPEED SCAN SPEED The Standard scan readout speed can achieve a frame rate of 120 fps for full resolution with low noise 0 43 electrons r m s and the Ultra quiet scan readout speed can achieve extremel...

Страница 33: ...1 External trigger mode Edge trigger Level trigger Global reset edge trigger Global reset level trigger 1 Vn2 Exp2 3 1H 4096 2304 119 2048 134 1024 266 512 524 256 1013 8 10 683 4 12 626 External trig...

Страница 34: ...modes Binning Calculation formula Hn Vn Max frame rate fps Free running mode 1 1 If Exp2 13889 1 Vn2 Vn5 1H Others 1 Exp2 1H 4096 2304 17 6 2048 2048 39 8 1024 1024 158 512 512 532 256 256 1044 256 8...

Страница 35: ...8 1152 47 1 4 4 1024 576 94 3 External trigger mode Edge trigger Level trigger Global reset edge trigger Global reset level trigger 1 1 If Vn2 Exp2 2 V4 1 Vn4 1 1H Others 1 Vn2 Exp2 3 1H 4096 2304 23...

Страница 36: ...1H Others 1 Vn2 Exp2 3 1H 4096 2304 35 3 2048 2048 79 5 1024 1024 266 512 512 524 256 256 1013 256 8 10 683 256 4 12 626 2 2 2048 1152 70 7 4 4 1024 576 119 External trigger mode Synchronous readout t...

Страница 37: ...3 4 826 External trigger mode Edge trigger Level trigger Global reset edge trigger Global reset level trigger 1 Vn2 Exp2 3 1H 4096 2304 4 99 2048 5 61 1024 11 1 512 22 0 256 42 8 8 526 4 643 External...

Страница 38: ...e setting 1 Standard scan Exp1 7 2 s to 1800 s input in units of seconds 1H 7 2 s Exp2 ROUNDUP Exp1 1H Round up to integer Calculation formula Exp2 1H 2 Ultra quiet scan Exp1 172 8 s to 1800 s input i...

Страница 39: ...he normal readout mode is suitable for observation monitoring field of view and focus adjustment and animation because it can operate the fastest frame rate with full resolution In case of standard sc...

Страница 40: ...r signal level when signal overflow happens due to too much input photons in normal readout mode In this mode the fastest frame rate is 120 fps Standard scan via CoaXPress 17 6 fps standard scan via U...

Страница 41: ...timing information 1 Edge trigger mode The Edge trigger mode is used so that the exposure starts according to an external signal Exposure time is set In this mode the exposure of the first line begin...

Страница 42: ...ge of all pixels at the same time Then all pixels can start exposure at the same time With this Global reset Edge trigger mode the exposure of all pixels begins on the edge rising falling timing of th...

Страница 43: ...mple below is for the trigger level High The exposure of the first and second lines begin when the trigger signal becomes High and the exposure of the third and fourth lines begin after the readout ti...

Страница 44: ...of all pixels at the same time Then all pixels can start exposure at the same time The example below is for the trigger level High With this Global reset Level trigger mode the exposure of all pixels...

Страница 45: ...ecuring as long exposure time as possible while controlling the exposure start timings by external trigger signals Normal operation when the Trigger Time is set as 1 The Synchronous readout trigger mo...

Страница 46: ...controlled by specifying the number of timing pulses to determine the exposure time The input trigger is valid only during the trigger ready is enabled The following figure shows the exposure timing...

Страница 47: ...the highest frame rate because it is operated in internal trigger mode In Start trigger mode the camera starts exposure and switches to internal trigger mode by the edge of an external trigger signal...

Страница 48: ...Note There is no output signal when the exposure time is less than the frame rate 2 Any row exposure timing output Global exposure timing output shows the global exposure timing where all lines expos...

Страница 49: ...s working in the Edge trigger mode the next frame can start after the previous frame exposure is done Thus the camera cannot accept a trigger for the next frame during the exposure period To reduce us...

Страница 50: ...top to the bottom line Figure 11 13 or from the bottom to the top line Figure 11 14 in Lightsheet Readout Mode Figure 11 13 Normal area mode Figure 11 14 Lightsheet Readout Mode 11 2 2 ABOUT READOUT...

Страница 51: ...Free running mode 1 Vn2 Exp2 2 1H 4096 2304 119 2048 134 1024 267 512 526 256 1021 8 11 574 4 13 888 External trigger mode Edge trigger 1 Vn2 Exp2 3 1H 4096 2304 119 2048 134 1024 266 512 524 256 101...

Страница 52: ...p2 Vn2 2 1H 4096 2304 17 6 2048 2048 39 8 1024 1024 158 512 512 526 256 256 1021 256 8 11 574 256 4 13 888 External trigger mode Edge trigger If Exp2 Vn2 2 Vn3 1 Vn3 1 1H Others 1 Exp2 Vn2 3 1H 4096 2...

Страница 53: ...67 512 512 526 256 256 1021 256 8 11 574 256 4 13 888 Note The frame rate value is valid 3 digits and rounded down to 4th digit Note The calculation formula and the frame rate value of Start trigger m...

Страница 54: ...Ver 1 0 54 11 2 5 TIMING DIAGRAM 1 Free running mode Figure 11 15 Ex Top to bottom readout 2 Edge trigger mode External trigger mode Figure 11 16 Ex rising edge Top to bottom readout Delay Jitter 4H M...

Страница 55: ...qCMOS Camera C15550 20UP Instruction manual_Ver 1 0 55 3 Start trigger mode Figure 11 17 Ex rising edge Top to bottom readout Delay Jitter 4H Maximum 1H Standard scan 1H 7 2 s to 237 6 s...

Страница 56: ...rammable timing output By using the programmable timing output synchronizing with external devices is simple A system which needs simple timing signal does not require a delay unit or pulse generator...

Страница 57: ...ose Hsync for the reference of programmable timing output camera can output some pulses before start the exposure It is called as Pre Hsync You can set the number of Pre Hsync 3 Trigger ready output T...

Страница 58: ...ime pixel correction features to improve image quality The correction is performed in real time without sacrificing the readout speed at all This function can be turned ON and OFF Default is ON User c...

Страница 59: ...8 bit digital output the amount of data can be the half of original 16 bit data and each pixel have 256 steps of gradation When you use USB 3 0 interface the frame rate can be faster with 12 bit and 8...

Страница 60: ...FF The master pulse supports free running mode start trigger mode and burst mode The range of interval time is 5 s to 10 s and the step is 1 s for the master pulse 1 Free running mode The camera gener...

Страница 61: ...ver center area of the window gently 3 Confirm whether dust is not left Attach the camera to an optics and check if there is dust or not under the uniform light condition If there is dust on the image...

Страница 62: ...m it is possible to use distilled water or deionized water by conducting periodical check However notice it increases possibility of corrosion inside cooling water path lowering flow rate or water flo...

Страница 63: ...asures Chapter Scratches or discoloration visible on the screen Lens is dirty Wipe the lens 12 Image is blurred Lens is not focused Contact a Hamamatsu subsidiary or your local distributor 17 Condensa...

Страница 64: ...43 electrons Ultra quiet scan 0 27 electrons Quantum efficiency 1 475 nm 90 900 nm 33 Full well capacity 1 7000 electrons Dynamic range 1 25 900 1 2 Dark current 1 Cooling temperature 20 C 0 016 elec...

Страница 65: ...s 1 s step Trigger times Synchronous readout trigger 1 to 10 000 Trigger output Global exposure timing output Any row exposure timing output Trigger ready output 3 programmable timing outputs High out...

Страница 66: ...Typical value 2 The water temperature is 20 C and the ambient temperature is 20 C 4 Power supply specifications Camera Input power supply DC 12 V Typical output Power consumption 84 W AC adapter Inpu...

Страница 67: ...nder the environment where condensation will not take place referring to the following graph 0 10 20 30 40 50 60 70 80 0 10 20 30 40 50 60 70 80 15 20 25 30 35 40 Ambient humidity Ambient temperature...

Страница 68: ...qCMOS Camera C15550 20UP Instruction manual_Ver 1 0 68 15 DIMENSIONAL OUTLINES 15 1 C15550 20UP Unit mm...

Страница 69: ...rs during the warranty period in the event of a natural disaster or if you handle the camera contrary to the instructions in this manual use it without due caution or try to modify it 3 We will repair...

Страница 70: ...jp Local contact information worldwide can be found at www hamamatsu com The contents of this manual are subject to change without notice The unauthorized duplication or distribution of all or part o...

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