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OSMOMAT 050 User Guide

Page 15 of 51

Version 1.1 (2011-10-12)

4 Preparing the Measuring cell for Measurement

To prepare the measuring cell, you need the tools and accessories included with the standard acces-

sories as well as a suitable solvent, distilled water and the prepared semipermeable membrane and

filter paper

(see chapter 4.4)

.

4.1 Making the Tube Connections during Initial Startup

The tubes included with the standard accessories are connected according to

Figure 4-1: Making the

tube connections

.

Figure 4-1: Making the tube connections

Use only the Tygon tubes supplied by the manufacturer. Other tubing material is per-

meable to air, which causes air bubbles that may severely impact or prevent the

measurement.

Bore for pressure

compensation

Ringer's solution

Supply bottle

plunger

Waste, minimum

fill level 1 cm

Waste bottle

plunger

Connection tube between

ground and waste bottle

Connection tube between

supply bottle and pump

Ground

Connection tube between measuring cell

and ground

Upper measuring cell

T screw

Lower measuring cell

Septum screw

Tube nipples

Connection tube between pump

and measuring cell

Tube pump

Pump tube

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Содержание OSMOMAT 050

Страница 1: ...www gonotec com User Guide Colloid Osmometer Oncometer OSMOMAT 050 Osmolality Measurement in medical and pharmaceutical field...

Страница 2: ......

Страница 3: ...marked as follows This symbol warns of the danger of corrupt ing measurement results for example by improper use of the measuring cell This symbol warns of the danger of damag ing the unit or the meas...

Страница 4: ...050 Colloid Osmometer 9 1 6 Packaging Contents 9 2 1 Overview of Display and Connectors 10 2 2 Power Supply 12 2 3 Dimensions and Weight 12 2 4 Safety and Handling Information 12 2 5 Known Risks Assoc...

Страница 5: ...cks 6 of Medical Devices Operator Ordinance 38 8 3 Measurement Checks 11 of Medical Devices Operator Ordinance 38 8 4 Internal Quality Control of the OSMOMAT 050 39 8 5 Replacing a Defective Power Fus...

Страница 6: ...tions The OSMOMAT 050 colloid osmometer determines the colloid osmotic pressure COP of blood plasma The main diagnostic considerations for determining the colloid osmotic pres sure in medical applicat...

Страница 7: ...ressure e g serum or plasma to the sample cell permeation of solvent causes underpressure in the reference cell based on the laws of osmosis because the membrane is impenetrable to proteins this can o...

Страница 8: ...ide Version 1 1 2011 10 12 1 4 Reproducibility of the OSMOMAT 050 Measurement display 3 digits Measuring range 73 mm HG 100 cm water column or 9 99 kPa Resolution 0 1 mm HG over the entire measurement...

Страница 9: ...the contents of your shipment are complete We cannot accept responsibility for any missing items reported at a later date Accessories and consumables included in the shipment Item number 20 9 0100 1 p...

Страница 10: ...AND BY OSMOMAT 050 Colloid Osmometer 23 2 Connection tube between supply bottle and upper half of measuring cell Bottle tray Supply bottle Waste bottle Connection tube between waste bottle and ground...

Страница 11: ...power supply Characteristics for device fuse to be used Manual pump control RS232 output Analog output Alignment potentiometer for analog port Serial number plate with information on serial number pow...

Страница 12: ...e performed Always switch on device when performing work on the measuring cell or the pressure trans ducer see chapter 3 Open measuring cell and remove membrane and filter paper Repeatedly rinse measu...

Страница 13: ...switched on and in calibration mode can be set by pressing the CALI BRATION button Excess pressure which may result in the destruction of the pressure transducer is indicated on the display This happ...

Страница 14: ...s solution and empty the waste bottle regularly If the device is left in standby mode for an extended period of time without topping of the Ringer s solution the measuring cell will dry out quickly w...

Страница 15: ...re 4 1 Making the tube connections Figure 4 1 Making the tube connections Use only the Tygon tubes supplied by the manufacturer Other tubing material is per meable to air which causes air bubbles that...

Страница 16: ...nd waste bottles are passed through a bore of the unscrewed cap and pushed onto the corresponding plungers Figure 4 2 The plungers hold the tubes on the bottom of the bottles The second opening on the...

Страница 17: ...contain any amount of solved gas air If necessary it must first be degassed by heating up just under the boiling point or by degassing under vacuum We recommend using the 30 mL plastic syringe includ...

Страница 18: ...pore size or pore size range is the typical factor for the retention and is called cut off The mem branes used for membrane osmometry usually have a cut off between 5 000 and 30 000 dalton The pore si...

Страница 19: ...rom a few seconds to an hour Next the membrane is completely submerged into the water and ready for installation into the mem brane osmometer for measurement of aqueous solvents It is important to ins...

Страница 20: ...use in the measuring cell Degassing the filter paper requires a lot of care because all air bubbles must be removed from the paper It is recommended to degase use to degas the filter paper together wi...

Страница 21: ...sounds stop unscrewing immediately After pressure com pensation the alarm is silenced and you can proceed to open carefully Loosen the tension screw flip back the clamping yoke and remove the upper h...

Страница 22: ...r bubbles form Next remove the water from the measuring cell using a paper tissue Figure 4 8 Adding the degassed solvent The lower half of the measuring cell is now cleaned and you can add the solvent...

Страница 23: ...f the measuring cell without air bubbles Remove the conditioned semipermea ble membrane from the water flip it once and place it on top of the filter paper in the measuring cell with the active side f...

Страница 24: ...ed together carefully in small intervals If the pressure increases to above 99 9 cm water column the display starts flashing Figure 4 12 If the pressure increases further to approx 550 cm water column...

Страница 25: ...semipermeable membrane becomes slightly transparent and a uniform somewhat dark ring is visible at the edge of the membrane Figure 4 14 The displaced Ringer s solution collected in the overflow chann...

Страница 26: ...performed directly by creating a hydrostatic pressure difference To fill all tubes without air bubbles press the Pumpe button at the back panel until the tube going to the waste bottle is also filled...

Страница 27: ...tube system has to be filled without air bubbles 3 Move the bottle from the tray to the table surface The height difference between tray and ta ble surface is exactly 10 cm Setting a stable measuremen...

Страница 28: ...es for Measurement The OSMOMAT 050 prepared according to item 4 and calibrated according to item 5 as well as a precision syringe with special cannula for injecting the sample included with the access...

Страница 29: ...ssible without significant changes to the colloid osmotic pressure How ever the sample should not be frozen 2 The minimum sample volume required is 150 L The samples must be homogeneous and should hav...

Страница 30: ...cannula through the septum as far as it will go It is impossible to puncture the membrane during this procedure Inject the sample into the measuring cell using three equal cycles of approx 50 L Fig 6...

Страница 31: ...ize in the lower measuring cell filled with solvent This balanced state is detected by the electronics and the measurement value is shown on the digital display Figure 6 6 At the same time the LED 2 a...

Страница 32: ...ristic see Page 34 Figure 6 9 After the RESULT LED and LED 3 light up the OSMOMAT 050 waits again for the same time elapsed from injection until display of the first measurement value to accept and st...

Страница 33: ...g to curve 2 If obtaining the result takes a very long time due to a sluggish adjustment characteristic curve 3 Fig ure 6 9 the measurement value is accepted after 4 5 minutes and the second measureme...

Страница 34: ...value 1st measurement value 2nd measurement value 2nd measurement value 1st measurement value 2nd measurement value Curve 3 Adjustment characteristic of the osmotic pressure when the pores of the semi...

Страница 35: ...oups identifying the function of each component its potential malfunctions the effects of the malfunctions on the measurement sys tem the possible causes of the malfunctions and the procedure for corr...

Страница 36: ...value is unsatisfactory mea surement value too low Pump tube too old flagged Part of the injected sample flows toward the pump System partially clogged between measur ing cell and waste bottle Strong...

Страница 37: ...aks from the tube connection be tween measuring cell and pump when in jecting the sample Entry of measuring cell is clogged Open and clean measuring cell Pressure transducer Device rinses the measurin...

Страница 38: ...inance 8 2 Safety Checks 6 of Medical Devices Operator Ordinance The OSMOMAT 050 is not listed in Annex 1 of the Medical Devices Operator Ordinance Public au thorities do not require the unit to under...

Страница 39: ...ctive power fuse unplug the unit from the power supply Risk of electric shock To replace the fuses use a small screwdriver to remove the fuse holder on the rear of the unit The two fuses can now be re...

Страница 40: ...gram is sent Idle level 3V U 7 5V Active level 3V U 7 5V DSR Dataset Ready input This signal can prevent transmission of a telegram The OSMOMAT 050 starts transmitting only after DSR becomes active Th...

Страница 41: ...with special cannula for septum injection 1 50 9 0130 Plastic bottles 250 mL supply waste bottles 2 50 9 0140 Tool for widening the tube ends 1 50 9 0150 Tweezers for membranes 1 50 9 0160 Plunger for...

Страница 42: ...atement OSMOMAT 050 Gonotec GmbH GSG Hof Reuchlinstr 10 11 D 10553 Berlin We hereby declare that the OSMOMAT 050 colloid osmometer complies with Directive 98 79 EEC Compliance is declared per Annex II...

Страница 43: ...nal warranty services such as on site service may be available from an authorized Gonotec sales partner B Limitation of the Warranty TO THE EXTENT PERMISSIBLE UNDER THE APPLICABLE LOCAL LAWS NEITHER G...

Страница 44: ...returning the equipment 4 If the equipment is being returned for credit you must include all accessories power cord software disks manuals etc 5 Return the equipment in its original packaging If you...

Страница 45: ...ibration direct pressure calibration through hydrostatic solvent col umn or using solutions of known concentration Measurement display 3 digit Recorder output 2 mV digit adjustable 10 for strip chart...

Страница 46: ...pen 3 The solvent added to the opened measuring cell flows downward through the pressure transducer The pressure transducer can be replaced by trained medical technicians by following these instruc ti...

Страница 47: ...50 Seite 47 von 51 Version 1 1 2011 10 12 13 2 Instructions 13 2 1 Disassembly 1 Unscrew and remove lower front panel 6 screws 2 Unplug pressure transducer connector from board 3 Release LED cable by...

Страница 48: ...housing 5 Remove the lower measuring cell and the large O ring 63 22 x 1 78 mm 6 Loosen the counter nut and unscrew and remove the pressure transducer from the measuring cell Remove the small O ring...

Страница 49: ...er is delivered with a cable tie and a new O ring For devices up to serial number 101122 where the pressure transducer is replaced for the first time the lower measuring cell must be replaced as well...

Страница 50: ...h cleaning solution and rinse it with water very carefully Install the new O ring 2 mm in the lower measuring cell 3 Screw the pressure transducer into the lower half of the measuring cell It may be n...

Страница 51: ...e the large O ring 63 22 x 1 78 mm in the lower half of the cell and reinstall the cell half into the housing from below using the two screws Insert the yellow LED into the designated bore and secure...

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