9
Release the push-button, smoothly. Eject the tip by
pressing firmly on the tip-ejector button.
6 - generaL guIdeLInes For good PIPettIng
Make sure that you op-
erate the push-button
slowly and smoothly.
When aspirating, keep
the tip at a constant
depth below the sur-
face of the liquid (refer
to the table).
Change the tip before
aspirating a different
liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the
tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should never enter the tip-holder; to prevent
this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, pre-
rinse the tip.
For volatile solvents you should saturate the air-
cushion of your pipette by aspirating and dispensing
the solvent repeatedly before aspirating the sample.
When the temperature of the liquid is different from
the ambient temperature, pre-rinse the tip several
times before use.
you may remove the tip-ejector (
see Chapter 11 -
Maintenance
) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids
that emit vapors, remove the tip-holder, rinse the
Table - Immersion Depth
and Wait Time
Model
Immersion Wait Time
Depth (mm) (seconds)
P2N 1 1
P10N 1 1
P20N 2-3 1
P100N 2-4 1
P200N 2-4 1
P1000N 2-4 2-3
8x20, 12x20
2-3
1
8x200, 12x200
2-3
1
