8
First stop
Second stop
aspirate
Press the push-button to the
first stop
(this
corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip in the
liquid (see immersion depth table, page 10). Release
the push-button slowly and smoothly (to
top
posi-
tion) to aspirate the set volume of liquid. Wait one
second (time depends on model, see table) ; then
withdraw the pipette-tip from the liquid. you may
wipe any droplets away from the outside of the tip
using a medical wipe, however if you do so take care
to avoid touching the tip’s orifice.
dispense
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the
first stop
.
Wait for at least a second, then press the push-button
to the
second stop
to expel any residual liquid from
the tip. keep the push-button pressed fully down and
(while removing the pipette) draw the tip along the
inside surface of the vessel.