Gilson F81013, Руководство пользователя

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USER’S GUIDE
Chapter 9
GUIDELINES FOR GOOD PIPETTING
Aspirate and Dispense
Fit new PIPETMAN DIAMOND Tips for the best results (refer
to Chapter 18
Specifications
on page 29).
Single Channel Model
For single channel models, push the tip holder into the tip
using a slight twisting motion to ensure a firm and airtight seal.
MultiChannel Model
PIPETMAN DIAMOND tips are best fitted with the
ROCKY RACK technique, invented by Gilson, available only
in our TIPACK and TOWERPACK.
Pre-Wet the Tips
All Models
Pre-wetting the tips before pipetting helps prepare the tips for the best
pipetting performance. Ideally, the pre-wet includes both immersing the tip
in the liquid and performing one pipetting step.
Pre-wetting the tips helps ensure
that volumes that you pipette will
achieve accuracy and precision within
specifications.
Hold the pipette vertically and immerse
the tip in the liquid (refer to page 14).
Press the push button to aspirate the set
volume of liquid. Wait a few seconds (time
depends on model, refer to page 14); then
withdraw the pipette tip from the liquid.
You may wipe any droplets away from
the outside of the tip using a medical
wipe; however if you do so, take care to
avoid touching the orifice of the tip.
Place the end of the tip against the inside
wall of the recipient vessel (at an angle of
10° to 40°). Press the push button. Wait
for at least a few seconds before releasing
the push button to expel any residual
liquid from the tip. While removing the
pipette draw the tip along the inside
surface of the vessel.
Eject the tip by pressing firmly on the tip
ejector button.
MODEL
IMMERSION
DEPTH (MM)
WAIT TIME
(SECONDS)
P10M 1
1
P20M 2 – 3 1
P100M 2 – 4 1
P200M 2 – 4 1
P300M 2 – 4 1
P1200M 2 – 4 2–3
P5000M 3
–
6 4–5
P10mLM 5
–
7
4–5
P8x10M/P12x10M 1 1
P8x20M/P12x20M 2 – 3 1
P8x100M/P12x100M 2 – 4 1
P8x200M/P12x200M 2 – 3 1
P8x300M/P12x300M 2 – 3 1
P8x1200M/P12x1200M 2
–
4 2–3
Table 1
Immersion depth and wait time
Chapter 9