Gilson F81013, Руководство пользователя

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8
DESCRIPTION
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PIPETMAN® M
1. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use
PIPETMAN DIAMOND Tips for assurance of accurate and precise results, refer to
Chapter 8 PIPETMAN DIAMOND Tips on page 13).
2. Press the push button to aspirate the selected volume.
3. To dispense: click (press and release) the push button.
Three things happen: sample dispense, automatic
purge, and piston reset to zero. Or, keep your thumb
pressing the push button until the end of the dispense
cycle: the piston reset takes place after releasing the
push button again. This feature enables you to remove
the tip from the liquid, without aspirating anything.
4. Eject tip by pressing the ejection button.
Your PIPETMAN M is ready for the next pipetting cycle while
maintaining the volume and the last settings.
Continue pipetting as you would with your mechanical PIPETMAN; it’s as simple as
that to use your new, electronic pipette!
REPETITIVE Mode
The REPETITIVE Mode allows you to distribute the same volume repeatedly in a
predefined number (N) of equal aliquots. You supply the aliquot volume
(AV) and the pipette automatically calculates the number of aliquots
possible from the nominal (maximum) volume (NV) of the pipette as
follows:
N = NV/AV (e.g., 120 μL x 10 for P1200M).
You also can decrease the number of repetitions (e.g., 120 μL x 10-(n ≥ 1) for P1200M).
NOTICE Always fit a tip before using any pipette.
START button
Optional STOP
0
V
Purge
Figure 2
PIPET Mode
NOTE
The REPETITIVE Mode is ideal for dispensing aliquots, e.g., dispensing
a PCR master mix into PCR tubes or 96–well, pipetting elution buffer for
DNA extraction, preparing a target for spectrometric analyses, distributing
loading buffer into samples, etc.
1. Press mode button to access system menu, where you can choose between
different pipette modes. Select
REPETITIVE Mode
by turning the push button.
To confirm your selection click on the push button.
2. Turn the push button half way, the screen will display:
“Click to
change volume.”
3. Press the push button; the volume on the display will start to blink, and you can
now adjust the volume.
Set the aliquot volume
1. Click the push button, set the volume by turning the push button and click again
to confirm your settings.
2. Set the aliquot number: the maximal number of aliquots has been calculated by
your PIPETMAN M. The number of aliquots flashes and you can decrease this
number (N – (n ≥ 1)) by turning the push button.
3. Press again to confirm your settings.