TEMPHION INSTRUCTIONS
Seametrics • 253.872.0284
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General Calibration Information
The TempHion should be calibrated before first use and
periodically thereafter. It should also be calibrated if moving
to a different sampling environment where readings will be
significantly different than the current environment.
Environmental conditions of turbulence and temperature
swings, as well as local likelihood for bio-fouling or
mineral deposition, can vary considerably from site to site.
Therefore, where the sensor is to be used for long-term
monitoring, it is recommended that the calibration be
initially checked frequently until a performance history is
established.
Aqua4Plus/Aqua4Plus Lite provides an easy-to-use
calibration calculator for performing one- or two-point in-
field calibrations. Two-point calibrations are more accurate
and should be used whenever possible. A Calibration Kit
is available from Seametrics, which includes a beaker,
pipette, measuring beaker, and stand.
Field calibration is performed on each channel separately.
To calibrate a specific channel, select Settings and
Configuration from the Configure Menu in Aqua4Plus
(click the Settings button in Aqua4Plus Lite), and then
click on the channel to be calibrated.
Step-by-step instructions will be displayed on the right
side of the window.
Note that the temperature channel rarely needs adjustment.
If you think your temperature channel needs calibrating,
contact your service representative.
Use the calibration calculator to perform one- and two-
point calibrations.
SETTINGS AND CALIBRATION
IMPORTANT CALIBRATION NOTES!
Data Files
• Calibration can only be done when
there is no data stored on the sensor.
Retrieve any data you want and then
erase the data before continuing.
Filling Solution Reservoir
• In order for the TempHion Smart
Sensor to calibrate and function
correctly, the filling solution reservoir
must be properly filled with reference
solution. For details, see Maintenance
section.
• Be sure the o-ring on the black
reference housing has been moved to
the lower groove, exposing the liquid
junction port.
Calibration Buffers and Solutions
• Refer to the following sections for
specific solutions and buffers.
• Important! The sensor and all
calibration buffers and solutions
should be at the same temperature
before and during calibration.
Reconditioning
• If your reference electrode has been
stored dry or in KCl solution, you
should let it sit at least a half hour
after filling with reference solution
before using or calibrating.
• If your pH sensor has been stored
with the bulb dry, you should soak it
in your first pH buffer for about four
hours to recondition the bulb before
calibrating.
• If your ISE sensor has been stored with
the element dry, you should soak it
in your first calibration solution for
at least a half hour before using or
calibrating.
