- 10 -
Introduction,
continued
Introduction,
continued
e
Stain
TM
Protein Staining System
www.genscript.com
System Components,
continued
System Mechanism
System Mechanism,
continued
- 9 -
e
Stain
TM
Protein Staining System
www.genscript.com
Features
Important features of the
e
Stain
TM
Protein Staining System are listed below:
·
Unique electric staining technology created for fast, reliable and efficient
protein gel staining in as few as 7 minutes.
·
User-friendly electric staining unit allowing for easy and convenient
procedures.
·
Consumable staining pads offering convenience without the need for
additional solutions and buffers.
·
High staining efficiency as conventional Coomassie blue staining method.
·
Compatible for use with various types of mini polyacrylamide gels.
Schematic mechanism of
e
Stain
TM
Protein Staining System showing the
movement of Coomassie blue dye:
The
e
Stain
TM
Protein Staining System is based on the electric staining concept
which utilizes the unique and patent-pending technology developed by GenScript.
To use the
e
Stain
TM
Protein Staining System for in-gel protein staining,
assemble the
e
Stain
TM
Protein Staining Pad with your pre-run gel on the
e
Stain
TM
Protein Staining Device. Similar to semi-dry blotting, the
e
Stain
TM
Cathode Pad
and
e
Stain
TM
Anode Pad act as ion reservoirs that contain the appropriate anode
and cathode buffers incorporated into the gel matrix. Simultaneously the
e
Stain
TM
Cathode Pad presoaked with proprietary cathode buffer containing CBB dye
R-250 or G250, also supplies negatively charged staining reagents. The high
electric force generated between graphite anode and titanium cathode allows
for rapid and directional movement of negatively charged Coomassie blue dye
reagents into the gel matrix to stain the proteins and also the homogenous
movement of the free staining reagents out of the gel matrix to destain the gel
matrix within only 7 minutes or less. Therefore the
e
Stain
TM
Protein Staining
System provides fast, convenient and reliable in-gel protein staining without the
need to prepare any solutions and buffers.
eStain
TM
Graphite Electrode
The
e
Stain
TM
Graphite Electrode serves as the replaceable anode of the
e
Stain
TM
Protein Staining Device. For the common use, one
e
Stain
TM
Graphite Electrode
(11mm) is pre-installed inside the
e
Stain
TM
Protein Staining Device to constitute a
complete electric staining unit. Each
e
Stain
TM
Graphite Electrode is produced with
special process and can be steadily used for 200 times of electric staining.
e
Stain
TM
Cathode Pad
e
Stain
TM
Anode Pad
Pre-run gel
E
E
E
E
CBB
dye
CBB
dye
CBB
dye
CBB
dye
CBB
dye
CBB
dye
Titanium Cathode
2e
-
+ 2H
2
O H
2
+ 2OH
-
Graphite Anode
4OH
-
- 4e
-
O
2
+ 2H
2
O
