- 12 -
Protocols
e
Stain
TM
Protein Staining System
www.genscript.com
- 11 -
e
Stain
TM
Protein Staining System
www.genscript.com
Recommendations
1. Check the Power Cord supplied with the unit to ensure that the cord is
compatible with the local socket format.
2. Place the
e
Stain
TM
Protein Staining Device on a level laboratory bench.
Keep the area around the device clear to ensure proper ventilation of the
unit.
3. For your safety: Position the device properly such that the power switch
and the AC inlet located on the rear of the unit are easily accessible.
4. Ensure the AC power switch is in the
Off
position.
5. Attach the power cord to the AC inlet and then to the electrical outlet.
Use only properly grounded AC outlets and power cords.
6. When the electrophoresis of your samples is almost complete, press the
power switch (located on the rear of the device) to turn
ON
the
e
Stain
TM
Protein Staining Device. The upper status light is on indicating you are
using staining mode. Digital Display shows the default parameters (000).
To obtain the best results follow these recommendations:
·
Wear gloves at all times during the entire staining procedures to prevent
contamination of pads and gels.
·
Avoid using expired
e
Stain
TM
Protein Staining Pads. Always use the pads
before the specified expiration date printed on the package.
·
Always keep the Ice Pack supplied with
e
Stain
TM
Device stored at -20
℃
or
below to facilitate cooling electrodes procedure.
Installing the
eStain
TM
Device
You are ready to use the
e
Stain
TM
Protein Staining Device for staining
applications.
Quick Reference Guide
Mode
e
Stain
TM
plugged in
e
Stain
TM
device and
staining pad assembled
Time selection
Run
End of run
Checking using
frequency
of graphite anode
Replacement of worn
graphite anode
Action
Connect
e
Stain
TM
Device to an electrical
outlet and power
switch is on
Place staining pads
on the device and close
lid
Press
Time
button to
select desired running
time
Press
Start/Stop
button
Automatic
Press and hold
Time
button for 2 seconds
Switch off the device
and replace the worn
graphite anode with a
new one
Sound
–
–
–
–
Continuous beeping
for 2 minutes
–
–
Light
Steady upper
light
Steady upper
light
Steady upper
light
flashing upper
light
Steady upper
light
Steady upper
and lower light
–
Display
Default
running
time (000)
Default
running
time (000)
Running
time
defined
Count down
time
Default running
time (000)
Staining times
the graphite
anode has
been used for
–
A quick reference guide for operating the
e
Stain
TM
Protein Staining Device
is provided below.
Introduction