- 16 -
Protocols,
continued
Protocols,
continued
e
Stain
TM
Protein Staining System
www.genscript.com
Assembling Staining
Stack with eStain
TM
Pads
and Pre-run Gel,
continued
- 15 -
e
Stain
TM
Protein Staining System
www.genscript.com
8. Pull back the handle bar to close the lid of
e
Stain
TM
Device while keeping
the bar vertical to the lid, and then return the bar to horizontal position.
Note: Gel Window is used as the spacer between anode pad and cathode pad to prevent
short circuit. The edges of the pre-run gel may also be covered by Gel Window and
will probably be stained to deep blue after running an electric staining program.
6. Select appropriate Gel Window according to actual size of the gel (see the
table below). Place Gel Window on the gel. Ensure that the Gel Window fully
cover the margin of the anode pad.
Pre-run gel
Gel with size 66 x 56mm to 80 x 60 mm
Gel with size <66 x 56 mm
Gel Window
Gel Windows 76 x 64mm
Gel Windows 66 x 56mm
Gel Thickness
0.75 mm
1.0 mm
1.5 mm
Running Time
6 minutes
7 minutes
9 minutes
7. Tear the sealing of the 1 x
e
Stain
TM
Cathode Pad package. Remove the
e
Stain
TM
Cathode Pad from the package and place it on the gel.
Performing electric
staining
2. Press the
Start/Stop
button to activate the electric staining program. The
running time begins to count down and upper status light keeps flashing
during the whole staining program.
After assembling the staining stack, perform electric staining as described
below.
1. Press the
Time
button to set appropriate running time based on the gel
thickness (see table below). If an undesired running time is set by mistake,
press
Reset
button to clear the wrong time, and then press again the
Time
button to choose the desired running time.
Note: During electric staining process, the graphite electrode may be heated to 60-70
℃
.
For continuous use and best results, it is necessary to cool down both graphite
anode and titanium cathode to room temperature before the next run
．
(see next page for cooling procedures)
