1. Check the Power Cord supplied with the unit to ensure that the cord is
compatible with local socket format.
2. Place the
e
Stain
®
2.0 Protein Staining Device on a leveled laboratory bench.
Keep the area around the device clear to ensure proper ventilation of the
unit.
3. For your safety: Position the device properly such that the power switch
and the AC inlet located on the rear of the unit are easily accessible.
4. Ensure that the AC power switch is in the
Off
position.
5. Open the closed lid of the
e
Stain
®
2.0 Protein Staining Device by pressing
the
Open
button. Place one or two pieces of Sponge Cushion in the anode
tank depending on gel thickness. For 1.5 mm gel, use one piece of Sponge
Cushion; for 0.75 and 1.0 mm gel, use two pieces of Sponge Cushion.
6. Insert the
e
Stain
®
2.0 Graphite Electrode into the anode tank as described
in Section "Replacing the
e
Stain
®
2.0 Graphite Electrode", then close the
lid of the device.
e
Stain
®
2.0 Protein Staining System
- 12 -
e
Stain
®
2.0 Protein Staining System
www.genscript.com
- 11 -
www.genscript.com
Protocols
Recommendations
Installing the
eStain
®
2.0 Device
Follow these recommendations for best results:
1.
Wear gloves at all times during the entire staining procedures to prevent
contamination of pads and gels.
2.
Avoid using expired
e
Stain
®
Protein Staining Pads. Always use the pads
before the specified expiration date printed on the package.
Protocols,
continued
Installing the
eStain
®
2.0 Device,
continued
Note: For a new eStain
®
2.0 Protein Staining Device, two pieces of Sponge Cushion and
one unit of eStain
®
2.0 Graphite Electrode have already been installed inside the
unit for immediate use. If staining 0.75 and 1.0mm gel, use one or two pieces of
Sponge Cushion; If staining 1.5mm gel, use one or zero piece of Sponge Cushion.
If use the gel of Invitrogen, must remove the bump at the bottom of the gel.
9. When the electrophoresis of your samples is almost completed, press
the power switch (located on the rear of the device) to turn
ON
the
e
Stain
®
2.0 Protein Staining Device. The right status light is on indicating
staining mode. The lower four digits of the digital display show the default
running time (00:00).
You are ready to use the
e
Stain
®
2.0 Protein Staining Device for staining
applications.
7. Pull out the waste tray from the right side of the device. Place a new
Absorbent Filter Paper inside the tray and then push the tray back in.
Note: After 20 times of electric staining, replace the used Absorbent Filter Paper with
a new one. One piece of new Absorbent Filter Paper is included in each box of
eStain
®
Protein Staining Pads.
8. Attach the power cord to the AC inlet, then, to the electrical outlet.
Use only properly grounded AC outlets and power cords.
Note:
After
20 times of electric staining, replace the used Sponge Cushions with new ones.
A pair of new Sponge Cushions are included in each box of eStain
®
Protein Staining
Pads.
