- 16 -
e
Stain
®
2.0 Protein Staining System
www.genscript.com
- 15 -
e
Stain
®
2.0 Protein Staining System
www.genscript.com
Protocols,
continued
Gel Thickness
0.75 mm
1.0 mm
1.5 mm
Running Time
6 - 7 minutes
7 - 8 minutes
7 - 8 minutes
Performing Electric
Staining
2. Press the
Start/Stop
button to activate the electric staining program. The running
time begins to count down and right status light keeps flashing during the whole
staining program.
After assembling the staining stack, perform electric staining as described below.
Perform electric staining within 15 minutes after assembling the pads with the gel.
1. Use the following parameters as starting points for electric staining based on gel
thickness.
Note: For best staining results, it is recommended to use eStain
®
2.0 Protein Staining System at
room temperature (22 - 28
℃
). If the ambient temperature is below 22
℃
, you may need to
extend the running time to obtain satisfied staining results based on gel thickness.
Press the
Min.
and
Sec.
buttons to set appropriate running time. If an
undesired running time is set by mistake, press
Reset
button to clear the
wrong time, and then press again the
Min.
and
Sec.
buttons to choose the
desired running time.
3. At the end of the staining, current automatically shuts off and the
e
Stain
®
2.0 Protein Staining Device signals the end of staining with repeated
beeping sounds. The right status light stops flashing and the lower four
digits show text (00:00).
4. Press any button on the control panel to stop the beeping.
5. Proceed to disassemble the stack and clean the device.
To obtain consistent staining results, disassemble the staining stack right
away after ending the staining procedure.
1. Open the closed lid by pressing the
Open
button.
2. Carefully separate the stained gel from the staining stack and proceed
to document the gel image.
3. Discard the used
e
Stain
®
Protein Staining Pad.
Note: Do Not reuse the eStain
®
Protein Staining Pad. Discard after each use.
4. Take the
e
Stain
®
2.0
Graphite Electrode out of the device, dry the contact
rods with a dry cloth or tissue paper. Take care not to lose the spring on
the rod located near the triangle symbols before placing back into the
device.
Caution: Do Not clean the eStain
®
2.0 Graphite Electrode immediately after ending the
staining program since the graphite electrode is still hot after a run. Wait for 3
to 5 minutes before proceeding to cleaning.
5. Clean the titanium cathode and the anode tank with a dry cloth or tissue
paper.
6. Replace the used Sponge Cushions with new ones after 20 times of
electric staining.
7. Replace the used Absorbent Filter Paper in the waste tray with a new
one after 20 times of electric staining.
At this point, the
e
Stain
®
2.0 Protein Staining Device is ready for another run.
If you are not using the device for a period of time, turn off the power switch
located on the back of the device, take the graphite anode out, and keep the
lid open to let any solution left in the holes for the contact rods dry out.
For any other repairs and services, contact
Technical Support
. Do not perform
any repairs or services on the
e
Stain
®
2.0 Protein Staining Device by yourself
to avoid damaging the device.
Disassembling and
Cleaning the
eStain
®
2.0 Device
Protocols,
continued
Performing Electric
Staining,
continued
