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Operation

3

Monitor UV-900 User Manual 18-1120-05 Edition AF

19

3.7

Autozero

The autozero function sets the detected absorbance to zero when the

OK

button is pressed. All three wavelengths are autozeroed. Autozero is

recommended after wavelength changes in a method and before the sample

is injected.
1 Select main menu

Autozero

, press

OK

. The normal absorbance value

display is then shown.

In UNICORN, Autozero is set with the instruction

AutoZeroUV

in

System

Control:Manual:Alarm&Mon

.

3.8

Storage and shut-down

Overnight:

The flow cell can be left filled with buffer.

Weekend and long term storage:

Flush the flow cell with distilled water and

then fill it with 20% ethanol.
The flow cell can also be stored dry by flushing as above with distilled water

and then blowing a compressed inert gas such as nitrogen (N

2

) through the cell.

Replace the red protective caps. Never use compressed air as this may contain

droplets of oil.

Autozero

CAUTION!

Do not allow solutions which contain dissolved salts, proteins or

other solid solutions to dry out in the flow cell. Do not allow particles to

enter the flow cell as damage to the flow cell may occur.

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Содержание Monitor UV-900

Страница 1: ...ÄKTA Monitor UV 900 User Manual GE Healthcare ...

Страница 2: ......

Страница 3: ...g Declaration of Conformity is available on request The CE symbol and corresponding declaration of confor mity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations descr...

Страница 4: ......

Страница 5: ...ge 13 2 7 Connecting the tubing 13 2 8 Mounting the cell holder cover 14 3 Operation 3 1 On off 15 3 2 Menu selection and settings 15 3 2 1 Menu selection 15 3 2 2 Return to main menu 16 3 2 3 Select value 16 3 3 Main menu overview 17 3 4 Setting lamp on off 17 3 5 Reading absorbance values 18 3 6 Setting wavelength 18 3 7 Autozero 19 3 8 Storage and shut down 19 3 9 Using an external chart record...

Страница 6: ...ycling 28 5 Trouble shooting 5 1 General 29 5 2 Faults and actions 29 5 3 Error messages 30 Reference information A Description 31 A 1 Instrument 31 A 2 Flow cell 32 A 3 Monitor principle 32 B Menus 34 B 1 Check menu 34 B 2 Setup menu 36 B 3 Setting and using the alarm timer 37 B 4 Service displays 37 B 5 Menu overview 38 C Technical specifications 39 D Accessories and spare parts 42 Short instruc...

Страница 7: ...nitor for use in liquid chromatography Monitor UV 900 features Variable wavelength for detection in the range 190 700 nm Up to 3 wavelengths can be monitored simultaneously Two alternative flow cells with path length 2 mm and 10 mm Accurate and reliable monitoring through self test self calibration and fiber optics Monitor UV 900 ...

Страница 8: ...ny item on the instrument WARNING The instrument uses high intensity ultra violet light Do not disconnect the optical fibers while the monitor is running CAUTION The flow cell must not be used at pressures above 2 MPa 20 bar 290 psi At higher pressures the flow cell may break WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves ...

Страница 9: ...autions The instrument should not be installed in a corrosive atmosphere or in an atmosphere where deposits are liable to form on the optical surfaces The instrument should be located in a place of low temperature variations away from heat sources draughts and direct sunlight Theinstrumentmaybeoperatedatnormalambienttemperaturesintherange 4 to 40 C The instrument should be installed on a stable la...

Страница 10: ...e the red protective caps from the inlet or outlet or the black protective caps on the optical fiber connectors 2 Slide the rear white clip on the cell holder to its inner position for a 2 mm cell and to its outer position for the 10 mm cell 3 Place the flow cell in the opening between the white clips The cell should be positioned with the text and serial number facing upwards and to the front Pre...

Страница 11: ...heminto thesocketsandtighteningthenutsfingertightusingthefiberdetachment tool supplied 4 Slide the rubber sleeves on the 2 optical fibers onto the connectors Make sure that the sleeves are pushed tight to the housing to prevent dust or fluid entering the connection Serial number Normal position Serial number Alternative position CAUTION Do not touch the tips of the optical fibers with your fingers...

Страница 12: ...ons as a short circuit may disturb the measurements 2 Set the recorder to 0 1 V input full scale 0 V offset 2 5 Connecting to communication link ThemonitorcanbeusedinÄKTAexplorerorÄKTApurifierandcontrolledfroma PC running UNICORN version 3 21 or higher using UniNet cables Analogue out 0 1V Remote UniNet 1 Mains On off λ1 Wire 1 Wire 2 λ2 Wire 3 Wire 4 λ3 Wire 5 Wire 6 CAUTION The mains power to th...

Страница 13: ...1 Make sure the on off switch is in the OFF position O 2 Connect a mains cable between the instrument and a grounded mains socket TheinstrumentisdeliveredwithbothEuropeanandUStypemains cables as standard Any voltage 100 240 V AC 50 60 Hz can be used The instrument contains no user replaceable fuse 2 7 Connecting the tubing 1 Remove the red protective caps from the inlet and outlet of the flow cell...

Страница 14: ...nual 18 1120 05 Edition AF 14 2 8 Mounting the cell holder cover The cover is a simple push fit onto the cell holder Two small lugs on the cover locatein holes atthe frontandrearofthecell holder Thecoveris thenlowered over the cell holder ...

Страница 15: ...s OK or wait for about 45 seconds to return automatically 5 Ifnosuchwarningmessagesappear thedisplayautomaticallyshowsthe Main operating menu after approximately 1 minute The instrument is ready to use All parameters are factory set to default values 3 2 Menu selection and settings 3 2 1 Menu selection A specific menu is selected by turning the front selection dial clockwise or counter clockwise W...

Страница 16: ...To decrease the value turn the dial counter clockwise Whensettingnumericalvalues thecursormoves uptothenextdigitifthedial is turned quickly in one direction to simplify entering large values The cursor moves back one place to the right every two seconds if the dial is not turned The text or numerical value displayed is accepted by pressing the OK button To cancel press the ESC button Sub menu 3 3 ...

Страница 17: ...language unit number etc See section B 2 Setup menu Set different timer options See section B 3 Setting and using the alarm timer 3 4 Setting lamp on off The lamp should be switched off when no measurement is made to conserve the lamp operating time The lamp is switched on when a run is started No warm up time is required 1 Select the mode changing menu 2 Switch the lamp on Run or off End by press...

Страница 18: ...set at any time 1 Select main menu Set Wavelength press OK 2 Select sub menu Set Wavelength λ1 press OK 3 Set the value press OK 4 The next wavelength λ2 is now shown Set the value or turn the wavelengthoff byturningthedialcounterclockwiseuntilthevaluepasses through190nm Ifonlyonewavelengthis tobeused λ2 andλ3 shouldbe set to off Press OK The first wavelength λ1 can never be set to off 5 Repeat st...

Страница 19: ...m Control Manual Alarm Mon 3 8 Storage and shut down Overnight The flow cell can be left filled with buffer Weekend and long term storage Flush the flow cell with distilled water and then fill it with 20 ethanol The flow cell can also be stored dry by flushing as above with distilled water andthenblowingacompressedinertgassuchasnitrogen N2 throughthecell Replacetheredprotectivecaps Neverusecompres...

Страница 20: ...Set the value and press OK This value determines where the zero absorbance level should be in relation to full scale on the recorder The instrument has an automatic overrange function If during a peak the monitor signal reaches the full scale value the signal will drop instantly to 0 V and give an accurate display of the peak starting from this position 3 9 2 Event mark Event marks can be set for ...

Страница 21: ...e averaging time should be as short as possible see the table below On delivery the averaging time is set to 2 56 s 1 Select menu Set Averaging Time press OK 2 Set the value press OK Use the fixed values between 5 12 and 0 08 s When monitoring more than one wavelength the recommended averaging time is a minimum of 1 28 s In UNICORN the averaging time is set with the instruction AveragingTime in Sy...

Страница 22: ...oving an old calibration setting If the instrument already has a calibrated cell length set that differs from the nominal length a warning message appears on the UV monitor display at start up Another message displaying the calibrated cell length also appears The display toggles between these messages The display automatically returns to the main operating menu after about 45 seconds or if pressin...

Страница 23: ...itch on the UV lamp see 3 4 Setting lamp on off 2 Select a main menu on the monitor display that shows wavelength 254 nm 3 Fill one of the supplied syringes with 1 5 2 ml of the 0 AU cm solution Make sure that there is no air in the syringe 4 FitthesyringeintheUnionLuerconnectorandinjectthesolution Do NOT remove the syringe 5 Wait until the monitored absorbance value is stable 6 Perform an Autozer...

Страница 24: ...The Set Calibr Length menu appears Press OK 2 Enter the Real cell length value from the Excel file and press OK 3 The Set UV Cell Type menu appears Check the cell type value If the value is correct turn the selection dial clockwise to go to next menu If the value has to be changed press OK to change it 4 At the Set Cell Serial No menu press OK 5 Enterthe serial number ofthecell see thelabelonthefl...

Страница 25: ...erval Action see procedures below Every 3 month Check the instrument Every 6 month or more often if required Clean the flow cell and optical fiber connectors WARNING Removeliquidordirtfromthesystemsurfaceusing aclothand if necessary a mild cleaning agent WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemi...

Страница 26: ...n Time If the lamp ontime is 4000 hours contact GE Healthcare for lamp replacement 4 3 3 Flip time The flip time is the time the monochromator stepper motor has been in operation 1 Select menu Check press OK 2 Select menu Check Flip Time If the flip time is 2000 hours contact GE Healthcare for maintenance 4 3 4 UV flow cell This menushows the setpath length and the serial numberofthe UV flow cell ...

Страница 27: ...nd flush the cell with distilled water 10 ml 4 4 2 Cleaning the optical fiber connectors Wipe with 30 isopropanol on lens paper 4 4 3 Instrument housing 4 5 Cleaning the system Wipe the surface regularly with a damp cloth Do not allow spilt liquid to dry on the instrument Remove dirt from the surface using a cloth and a mild cleaning agent Let the system dry completely before using it Wipe the ins...

Страница 28: ...This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment ...

Страница 29: ...water There may be air in the flow cell Check that the flow restrictor gives a back pressure of 0 2 MPa in ÄKTAexplorer and 3 5 MPa in ÄKTApurifier If there is a lot of air in the water degas the buffer continuously we recommend helium sparging Check the connections of the UV cell optical fibers Clean the UV cell see section 4 4 Ghost peaks Air in the eluents Degas if necessary we recommend helium...

Страница 30: ... back to on or choose Recalibrate in the Check menu Change lamp Call GE Healthcare for Xe lamp exchange Mode fiber failure Reference fiber failure Lamp module failure Low light intensity Block filter is defect Call GE Healthcare High intensity in Reference fiber Recalibrate by switching the instrument off and on High intensity in Sample fiber 1 Disconnect the optical fiber connectors 2 Recalibrate...

Страница 31: ...s only Connector switch Function Analogue out 0 1 V Recorder output 3 channels 0 1 V Remote Digital signal inputs lamp on off autozero event mark UniNet 1 Computer network Mains Supply voltage grounded On off Instrument on off switch Monitor UV 900 Analogue out 0 1V Remote UniNet 1 Mains On off 1 2 3 4 5 6 7 8 9 Pin Signal Function Active status low or closed terminal to pin 5 0V 1 Remote on off A...

Страница 32: ...filter entrance slit and a concave aberration corrected holographic grating Monochromatic light from the grating is directed to an optical fibre The grating is turned by a stepping motor for wavelength selection between 190 700 nm in steps of 1 nm Up to 3 wavelengths can be monitored simultaneously For wavelengths between360 700nm a blocking filter is moved into the light path to filter out unwant...

Страница 33: ...able and because the optical unit is located away from the lamp and electronics noise and drift caused by temperature variations is avoided At calibration the instrument automatically finds 2 persistent lines in the spectrumofXenon Thewavelengthsoftheseknownlinesareusedtocalibrate the stepper motor that turns the grating Detectors and electronics Flash lamp 100 Hz Monochromator Beam splitter S R C...

Страница 34: ...ter approx 20 seconds the message Wavelength OK or Wavelength not OK isshowntogetherwiththedeviationvalues IfnotOK recalibrationcanbe selected If a deviation in wavelength occurs repeatedly when checked contact Amersham Biosciences Recalibrate If the instrument is left switched on for a long period 10 days it may be necessary to recalibrate it This calibration is identical to that done when the in...

Страница 35: ...isplay 2 Select sub menu Check Service Mode by pressing OK 3 The service telephone number is displayed Turn the selection dial clockwise to view the next display 4 Theservicecontractnumberisdisplayed Turntheselectiondialclockwise to view the next display 5 The instrument serial number is displayed Turn the selection dial clockwise to view the next display 6 The instrument name and software version...

Страница 36: ... It should correspond to the number set in UNICORN for the UV monitor The number should be set to 0 if one UV monitor is used If more than one UV monitor is used they must all have different identification numbers 1 Select main menu Setup press OK 2 Select sub menu Setup Unit Number press OK 3 Select unit number 0 25 Setup display angle The display angle can be set to compensate for different view...

Страница 37: ...s OK to enter the countdown value in the form HH MM SS pressing the OK button after entering each time unit 5 PressESCbuttontoreturntotheAlarm Timermenuwhichnowshowsthe set alarm time or count down time as BzzHH MM SS 6 Whenthealarm timeis dueorthe count down timerreaches 00 00 00 an alert display is shown and the instrument beeps until the OKbutton is pressed The alarm timer is based on the inter...

Страница 38: ...4 Set Wavelength λ2 215nm 260 Set Range 2 000AUFS 0 01 Set zero Level 10 of FS 20 Set Averaging Time 2 56s 2 56 Check Setup Checking Wavelength Please wait Wavelengths OK 230 0 459 0 542 0 OK Wavelengths not OK Recalibrate OK Recalibrating Please wait Recalibration finished OK Check Analogue Out off on Setup Unit Number 0 Eventmark λ3 215 0 00000 AU 280nm 254nm 215nm 0 000 0 000 0 000 Set Clock 00...

Страница 39: ...0 5 AU at 230 nm 6x10 5 AU at 230 nm 2x10 4 AU at 230 and 254 nm 2x10 4 AU at 230 and 254 nm Drift2 2x10 4 AU h at 254 nm Environment 4 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure 1 Measured with water at 1 ml min time constant 1 s 2 Typical values at room temperature after varm up Flow cell Max flow rate 100 ml min Max pressure 2 MPa 20 bar 290 psi Backpressure M...

Страница 40: ...240 V AC 50 60 Hz Analogue output 3 signals 0 1 V full scale overrange function Digital inputs 5 V 1 mA current sinking lamp on off autozero event mark Display 2 rows with 20 characters each Dimensions H xW xD 200 x 260 x 370 mm Weight 8 5 kg Degree of protection IP 20 Compliance with standards The declaration of conformity is valid for the instrument only if it is used in laboratory locations use...

Страница 41: ...he following harmonized standards EN 61326 emission and immunity EN 55011 GR 2 Class A emission This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation ...

Страница 42: ...8 1112 51 Union 1 16 female M6 male 6 18 1112 57 Union M6 female 1 16 male 8 18 1112 58 Union 1 16 male 1 16 male i d 0 25 mm 2 18 1120 92 Union 1 16 male 1 16 male i d 0 50 mm 2 18 1120 93 PEEK tubing i d 0 15 mm o d 1 16 2 m 18 1156 59 PEEK tubing i d 0 25 mm o d 1 16 2 m 18 1120 95 PEEK tubing i d 0 50 mm o d 1 16 2 m 18 1113 68 PEEK tubing i d 0 75 mm o d 1 16 2 m 18 1112 53 PEEK tubing i d 1 ...

Страница 43: ...to the installation instructions 1 Switch on the module with the mains switch on the rear panel 2 Switch on the UV lamp by selecting Run 3 Select wavelength for one two or three wavelengths 4 Autozero the module by pressing OK 5 The main operating menu RUN menu is shown Monitor UV 900 Select OK ESC Calibrating Please wait End Press OK to Run Set Wavelength 215 254 280 nm Set Wavelength λ1 215nm Se...

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Страница 46: ...lthcare AB Sanken Bldg 3 25 1 Huakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Paci c Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 57606 1619 Fax 01 57606 1627 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 800 567 1008 Central East South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45 16 24...

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