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X-Cite
®
200DC User Guide
035-00446R rev. 4
9.2 Liquid Light Guides
Tips to Prevent Premature Degradation of Light Guides
9.2.1 Liquid light guides have a typical useful life of 2-3 years/4000 operational hours
in the X-Cite
®
200DC when installed and handled properly. The formation of
bubbles is one of the most common reasons for a light guide to degrade
prematurely and result in a sudden reduction in illumination intensity. Bubbles
form without warning, usually due to overheating and/or mechanical stress to the
light guide. Below are some simple tips to avoid overheating and stressing the
light guide.
9.2.2 The light guide has a minimum bend radius of 1.6 inches (40.0mm) however, a
bend radius of at least 75mm is recommended for a light guide while “in use”.
Sharper bends can cause heat to build up and cause problems longer term.
Bending or coiling the light guide tighter than this radius will result in permanent
damage to the light guide
.
Minimum bend radius to prevent immediate damage to
the LLG is 40mm,.
9.2.3 Make sure the light guide is cooled properly during use, and prevent overheating:
a. Always fully insert the light guide in to the X-Cite
®
200DC; this ensures
contact with a heat sink to conduct heat away from the light guide.
b. Never obstruct the air vents on the X-Cite
®
200DC. Vents are located at the
rear and underside of the unit.
c. Do not remove the rubber feet on the X-Cite
®
200DC or otherwise
reduce/block the space between the bottom of the unit and bench top. This
may compromise airflow through the unit.
d. Ensure that the air being used to ventilate the X-Cite
®
200DC is
approximately “room temperature” (e.g. do not place the X-Cite
®
unit on top
of another heat-producing instrument).
e. If a heated environmental chamber is being used for live cell imaging, make
sure that the X-Cite
®
200DC and the light guide are located outside of the
chamber.
9.2.4 Do not expose the light guide to extreme temperatures (above 35°C, below -5°C)
for extended periods of time during use, transport or storage; this may cause
degradation of the seals and allow air bubbles to form in the liquid.
9.2.5 Never kink, bend, crush, or stretch the light guide; this type of mechanical stress
may cause bubbles to form in the liquid and/or damage to the outer sheath
a. Always allow adequate clearance between the light guide port of the X-Cite
®
200DC and other objects to prevent excessive bending.
b. Place the X-Cite
®
200DC close enough to the microscope so that there is
some slack in the light guide and no sharp bends.
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