
Rev. B 2022/02
67
CyBio FeliX
Operation
7.6.7
Pipetting
Each piston movement creates a negative or positive pressure during suction or discharge.
In order to achieve the corresponding precision, the pressure equalization in the tips must
be waited for.
In terms of the program, the "Pause" command is used for this purpose. The time required
to reach this state depends - among other factors - on the properties of the liquid being
handled. A waiting time of two seconds proves long enough with most watery solutions.
A complete volume cycle contains the commands "Suction", "Discharge" (partial volumes if
necessary), the blow-out (combined with "Discharge" or separately) and the command
"Piston in zero position". Two methods are distinguished for a liquid transfer - pipetting of
a single volume and reverse pipetting.
Pipetting (single volume)
Perform the following steps:
1.
Suction without overstroke.
2.
Discharge with residual discharge.
3.
Move to the zero position (without liquid).
Fig. 43:
Course of events
NOTE
Dangerous situation!
Always move to zero position without liquid to avoid damage!
Содержание Analytik Jena CyBio FeliX
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