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Rev. B 2022/02

Operation

CyBio FeliX

7.6

Software-controlled operation

The CyBio FeliX is controlled by a software (CyBio Composer), which allows the simple and

fast compilation of specific laboratory routines

(Explanations - see the following table, the following sections or the Help menu of the

software).

7.6.1

General commands (overview)

Sym-

bol

Function

Explanation

Ansaugen

Aspirate

• A volume is aspirated. The aspiration vol-

ume indicates how much liquid is to be
aspirated during the cycle. The permissi-
ble volume range depends on the head
type and tips used

• Optional overstroke volume is possible

Ausstoßen

Dispense

• The volume is discharged normally or with

residual discharge/blow-out.

Kolben in Nullposition

Piston to zero position

• Pistons are moved to their zero position

Spülen/Mischen

Rinse/Mix

• Rinsing the pipette tips or

mixing a volume

Pipettiergeschwindigkeit

Pipetting speed

• Setting the pipetting speed

Überwachung

Supervision

• Volume cycle monitoring

Deckposition anfahren

Move to deck position

• X and Y axis movement with respect to the

specified deck position

• Reference point of the Z axis corresponds

to
absolute zero

• (Switch reference point between absolute

zero and deck position reference*)

Labware anfahren

Move to labware

• X, Y and Z axis movement in relation to

the labware

• The reference point of the Z axis is the

bottom or top of the well of the respective
plate

Vertikalantrieb

Vertical drive

• Movement of the pipetting head in the Z

direction

Содержание Analytik Jena CyBio FeliX

Страница 1: ...CyBio FeliX Operating instructions...

Страница 2: ...may be dupli cated photocopied saved to a storage system or transferred to electronic media without prior written permission of the pub lisher Manufacturer Analytik Jena GmbH Konrad Zuse Stra e 1 077...

Страница 3: ...Rev B 2022 02 III CyBio FeliX...

Страница 4: ...IV Rev B 2022 02 CyBio FeliX...

Страница 5: ...ective 2006 42 EC In addition the product is in conformity with the EC Directive relating to electromagnetic compatibility 2014 30 EU The safety objectives of the Low Voltage Directive are taken into...

Страница 6: ...addition the product is in conformity with the EC Directive relating to electromagnetic compatibility 2014 30 EU The safety objectives of the Low Voltage Directive are taken into account Harmonized S...

Страница 7: ...11 CAN CSA C22 2 No 61010 1 2012 A1 2018 11 UL 61010 2 081 2019 CAN CSA C22 2 No 61010 2 081 2019 This product was voluntarily tested to the relevant safety requirements referenced on this certificat...

Страница 8: ...VIII Rev B 2022 02 CyBio FeliX...

Страница 9: ...ion Installation 17 3 8 Safety Notes for Operation 18 3 8 1 General 18 3 8 2 Explosion Protection Fire Prevention 18 3 8 3 Electrics 18 3 8 4 Basic Maintenance Care 18 3 8 5 Handling of Dangerous Subs...

Страница 10: ...7 6 2 Attach remove transport lock 58 7 6 3 Preparation of head change switch off 59 7 6 4 Movements in X Y and Z directions 60 7 6 5 Tip pick up change pipetting head T 62 7 6 6 Tip pick up change pi...

Страница 11: ...arts 87 10 1 Accessories 87 10 1 1Pipette tips 87 10 2 Pipetting Heads 88 10 3 Liquid handling CHOICE adapter 89 10 4 Holder 89 10 5 Further Accessories 90 10 6 Spare parts 90 11 Disposal 91 11 1 Cons...

Страница 12: ...XII Rev B 2022 02 CyBio FeliX...

Страница 13: ...51 Fig 24 Head retainer clamping lever swiveled forward 51 Fig 25 Axes of motion 52 Fig 26 Commands 58 Fig 27 Settings Set tool information 58 Fig 28 Settings for depositing 58 Fig 29 Component windo...

Страница 14: ...XIV Rev B 2022 02 CyBio FeliX...

Страница 15: ...yze possible causes of malfunc tions and determine suitable measures for eliminating them The operating instructions must be accessible to the operating and maintenance personnel at all times 1 2 Targ...

Страница 16: ...the following table for the design of the cross references Table 1 Design of the cross references Type of cross reference Display Comment Reference to an illustra tion see Fig 4 Illustration is locat...

Страница 17: ...rdous situation which if not prevented can result in death or most serious injuries CAUTION Dangerous situation Potential consequences Mild or minor injuries NOTE Dangerous situation Potential consequ...

Страница 18: ...cal laboratories In the fields of medicine and diagnostics its use is limited to research The basic functions are to aspirate and dispense fluids into and out of microplates reser voirs columns indivi...

Страница 19: ...ent and the conduct of personnel handling substances of this nature and on the practices in place for cleanness sterilization environmental protection and waste disposal For the use of the device with...

Страница 20: ...of damage Warranty and liability claims for personal injury and property damage are excluded if the device is not operated in accordance with section Conforming Use on page 4 1 7 Scope of delivery Th...

Страница 21: ...height x depth approx 650 x 665 7001 x 450 mm Weight Basic unit 30 5015 100 24 Device ready for operation approx 50 kg approx 58 kg Microplate specifications Formats SBS standard 96 384 shallow well M...

Страница 22: ...ight x depth approx 650 x 6451 6652 x 450 mm Weight 30 5015 500 24 30 5015 401 24 approx 39 kg approx 50 kg Microplate specifications Formats SBS standard 96 384 shallow well MP 96 384 deep well MP Tu...

Страница 23: ...041 1 High Breaking Capacity Fuse Buerklin 42 G 1786 Current consumption 2 A Overvoltage category II Degree of contamination 2 Interfaces Sub D 9 pin socket RS 232 IN Sub D 9 pin plug RS 232 forwardin...

Страница 24: ...10 Rev B 2022 02 Technical Specifications CyBio FeliX...

Страница 25: ...efective state or with safety and protection devices installed in a nonconforming manner Operate the device only with the mains voltage that corresponds to the information on the label Observe prescri...

Страница 26: ...n machinery and the EC declaration of conformity has been issued in accordance with appen dix II A 2 All safety data refer to the currently valid regulations of the European Union Other specific natio...

Страница 27: ...components and must be strictly observed Before switching on the device check that warning notes and safety symbols are com plete and intact Do not put the device into operation if warning notes or s...

Страница 28: ...particular way 2 If necessary refer to EN 166 Personal eye protection specifications 3 If necessary refer to EN ISO 374 Protective gloves against dangerous chemicals and micro organisms Warning note...

Страница 29: ...Rev B 2022 02 11 CyBio FeliX Safety instructions Fig 2 Warning signs on the device Fig 3 Warning sign on pipetting head...

Страница 30: ...motor driven displacement in X and Z direction 2 Deck movements motor Y displacement Observe the following general instructions Do not place your hands or fingers including objects you are holding int...

Страница 31: ...tential dangers that emanate from sub stances being processed If necessary appropriate body protection equipment must be used Before a break in operation or on completion of work adequate measures sho...

Страница 32: ...electrical and electronic parts of the system and its components may only be carried out by a suitably qualified electrician according to latest binding electrical regulations Never start up the devi...

Страница 33: ...uals and property when handling radioactive infec tious toxic caustic flammable and other hazardous substances For operating the device with hazardous substances the operator is recommended to elabora...

Страница 34: ...bstances compatible with the materials listed The following components have direct contact with the processed substances Table 7 Components Component Material Pipette tips PP Piston seals1 PE HD Reage...

Страница 35: ...Ozone Oxidative solutions Sodium hypochlorite Halogens Highly concentrated alkaline solutions 1 This table is not exhaustive Disinfection method Disinfectant1 Comment Spray disinfection Not applicabl...

Страница 36: ...00 ALPAQUA MAGNUM FLX Enhanced Universal Magnet Plate OL3317 11 285 Waste box I small 844 00430 0 Tip transfer tool 96 250 l DW filter 96 1000 l OL3396 352 25 OL3396 25 354 TipRack 96 1000 l OL3317 11...

Страница 37: ...e that the following requirements are met The operating staff must be familiar with the location of safety equipment accident and danger alarms as well as first aid and rescue equipment as well as the...

Страница 38: ...20 Rev B 2022 02 Safety instructions CyBio FeliX...

Страница 39: ...ting status indicator on page 31 6 Head mount Head mount on page 33 7 Bar code reader port see Accessory instructions 8 Head locking lever Pos 3 see Fig 14 on page 33 9 Type plate Type plate on page 2...

Страница 40: ...Connection value label Overview operating data conditions on page 9 8 Electrical connections Connector panel on page 35 9 Type plate Type plate on page 27 10 LED operating status indicator and button...

Страница 41: ...from a hazard Danger areas on page 12 from mov ing assemblies as well as from samples chemicals etc Optional addition to the enclosure is a decontamination device UV The louver is made of transparent...

Страница 42: ...zine as well as all types of micro plates and reservoirs in SBS format Marking of conforming use Movements are always performed without force acting on the upper decks In the case of receiving dispens...

Страница 43: ...and the following table for detailed information Table 10 Compilation NOTE Dangerous situation potential consequences Material damage If no height observation adjustment is performed there is the dan...

Страница 44: ...able for detailed information Table 11 Compilation Deck Heigh t Description Remarks A 115 mm Height 110 mm Usable height accessory B a C 70 mm Height 65 mm Usable height labware for all tip types exce...

Страница 45: ...e when the louver is closed Table 12 Red green LED Table 13 Yellow LED Indicator Comment GREEN1 The device is operational It can be operated via the PC GREEN FLASHING Device is in operation and runs a...

Страница 46: ...The upper LED is an operating status indicator the lower LED has an additional button function for manual operation Table 14 Upper LED Table 15 Lower LED button Indicator Comment GREEN The device is...

Страница 47: ...Dovetail guide 2 Electrical connection 3 Head locking lever Note Swivel the head locking lever Pos 3 see Fig 14 forward if no pipetting head is attached the pipetting head may only be pushed on in th...

Страница 48: ...yBio RoboTip Trays and liquid handling adapters CHOICE head The pipetting head has a mounting mechanism for automatic mounting of CHOICE adapt ers Additional information on the design and mode of oper...

Страница 49: ...onnectors and interfaces are located at the side of the device Fig 16 Connectors 1 USB socket type B 2 Sub D socket 9 pin COM IN 3 Network socket Fig 17 Connectors 1 Sub D plug 9 pin COM OUT 2 M8 4 pi...

Страница 50: ...PC ETHERNET Network connection 1 Communication interfaces TIP Consult the manufacturer regarding dedicated interfaces see Fig 17 see Fig 17 on page 351 Description COM OUT Forwarding interface to ano...

Страница 51: ...llowing operating modes are possible PC control automatic Software controlled operation on page 56 Manual mode control of the Z axis by means of a button Move the pipetting head in the Z direction on...

Страница 52: ...38 Rev B 2022 02 Technical description CyBio FeliX...

Страница 53: ...ystem components Adequate precautions should be taken to protect all components from environmental impacts mechanical shock or formation of condensed water during transportation or shipment Temporary...

Страница 54: ...re fastened 1 Check that the head mount is in the cen ter 2 Check that the lower deck is pushed to the rearmost position and the upper decks are fully apart 3 Insert transport lock of the decks 4 Inse...

Страница 55: ...longer period of time it should preferentially be stored in its original packing case The following requirements are placed on the climatic conditions in the storage room of the device Temperature ran...

Страница 56: ...42 Rev B 2022 02 Packing transport and storage CyBio FeliX...

Страница 57: ...ct exposure to sunlight and radiation emitted by heaters Provide air condi tioning for the room if necessary Always ensure free accessibility to the device and do not under any circumstances obstruct...

Страница 58: ...pted there is risk of fatal injury due to electric shock Never connect the mains plug of the device to a mains socket without a protective earth contact Make sure that the protection effect is not ren...

Страница 59: ...el authorized by them Initial start up essentially includes Installation and adjustment of the device components Providing cable connections and connecting power supply cables Software installation fa...

Страница 60: ...eak Test Leak testing is required in order to make sure that the pipettor head is free from points of leakage The test is performed by aspirating a specified volume of dye solution into the pipette ti...

Страница 61: ...r LED Red flashing on continuously An internal error is signaled Error analysis LED flashing red on page 73 Green on continuously Completion of initialization display of readiness for opera tion 5 Mak...

Страница 62: ...quid from tips Switch off the device and switch it on again after a short pause if necessary restore the operational readiness of the PC and start an empty CyBio Composer method The device initializes...

Страница 63: ...et of 30 mm starting from the top vertical position for replacing the head CAUTION Dangerous situation Risk of crushing as the louver1 is opened and closed 1 Does not apply to the version without encl...

Страница 64: ...ward Fig 19 Head mount clamping lever swiveled forward 3 Insert the pipetting head Fig 20 Push on the pipetting head 4 Secure the pipetting head i e swivel the clamping lever back Fig 21 Pipetting hea...

Страница 65: ...en louver1 3 Swivel the clamping lever forward Fig 22 Pipetting head fastened 4 Remove the pipetting head Fig 23 Take off the pipetting head Fig 24 Head retainer clamping lever swiveled forward 5 Clos...

Страница 66: ...electric adjustment options When moving manually pay attention to the following Movement is possible in off and on state Movement only with a moderate application of force Proceed with caution The ele...

Страница 67: ...tton 4 Press or and hold observe the pipetting head is moved Pipetting head is moving to the top vertical position down to the end position Stop pipetting head 1 Release or To reactivate the typing se...

Страница 68: ...mode LED button ver sion Only possible when the louver2 and or the external E STOP are open The Adjustment mode function is intended for software controlled safe operation with the louvers open e g t...

Страница 69: ...s are available for column by column work For working row by row the tips should be positioned at positions 1 2 or 3 Deck B is available for microplates and reservoirs Working by row or by line is pos...

Страница 70: ...discharged normally or with residual discharge blow out Kolben in Nullposition Piston to zero position Pistons are moved to their zero position Sp len Mischen Rinse Mix Rinsing the pipette tips or mi...

Страница 71: ...zeuginformation setzen Set tool info Selection of the components attached to the pipetting head or selection of the tip type in combination with liquid handling adapter Antriebsgeschwindigkeit Drive s...

Страница 72: ...llows with the help of the software 1 Check that the device and PC are switched on 2 Check whether the pipetting head is inserted correctly 3 Select the Unload command 4 Set a position on deck A and a...

Страница 73: ...switch off Perform the following steps 1 Select the Load command Fig 29 Component window 2 Perform the following configuration Fig 30 Attach transport lock NOTE Dangerous situation Insert the transpo...

Страница 74: ...s top vertical position Offset adjustable for all axes Fig 31 Window Positioning Move to labware Select position as reference point of X Y and Z axis Z axis Reference point Well bottom or Well top Off...

Страница 75: ...negative value results for X e g 3 5 mm For the back ward movement Y becomes positive e g 3 5 mm When these coordinates are entered the pipette tips are positioned at the upper left edge Depending on...

Страница 76: ...ghten the tips or the transport lock this is done with the Load and Unload commands Only the corresponding CyBio TipTrays are used see chapter Accessories in the pipetting head instructions Perform th...

Страница 77: ...th CHOICE adapters Only processing by column and line or single channel processing of microplates is possible To accommodate a liquid handling adapter a suitable support must be positioned on deck A w...

Страница 78: ...icking up an adapter the information about the pipette tips attached to the adapter picked up must be set as follows 1 Select the command Setting tool info 2 Make a selection under the appropriate ada...

Страница 79: ...s to pipette tips with filters filter tips where a small net volume may be available Tip type Description CyBio TipRack 96 1000 l 1 8 12 channel CHOICE adapter 10 l 1000 l CyBio TipBox 96 250 l 1 8 12...

Страница 80: ...ep the pipette tips and then if necessary the adapter is to be depos ited In both cases the Unload command must be selected When you deposit tips the adapter type or in case of an adapter the head typ...

Страница 81: ...waiting time of two seconds proves long enough with most watery solutions A complete volume cycle contains the commands Suction Discharge partial volumes if necessary the blow out combined with Discha...

Страница 82: ...urplus volume be taken and dispensed back into the source Perform the following steps 1 Suction with overstroke 2 Discharge single volume or subvolumes 3 Residual discharge of the overstroke volume 4...

Страница 83: ...y Any warranty claims shall lapse imme diately WARNING Danger due to electrical voltage Observe the safety rules when troubleshooting cables Error Cause Remedy Despite connectionto the mains socket th...

Страница 84: ...roperly inserted Insert the pipetting head cor rectly Start an empty software method for initialization If the error still occurs contact your ser vice partner or the manufacturer E 7 Internal error C...

Страница 85: ...ff the device Restart the device and start an empty soft ware method for initialization Restart the method for attaching the LH adapter make sure it is positioned correctly If the error still occurs c...

Страница 86: ...Check that there is no voltage on the device Name Type Value1 Mains fuse T 4 A 250V AC 5x20 mm 1 High Breaking Capacity Fuse Details Overview operating data conditions on page 9 1 Switch off the devi...

Страница 87: ...up is possible 7 12 4 Manual axis movement after an abort The pipetting head can be moved manually in the X direction with little effort Likewise the decks can be moved manually while the device can...

Страница 88: ...74 Rev B 2022 02 CyBio FeliX...

Страница 89: ...d adjustment of system modules under electrical voltage may only be carried out by a qualified electrician CAUTION Material damage to electrical and electronic components due to penetrating liquid Mak...

Страница 90: ...levels and hence an increased probability of failure Check for signs of wear and tear on assemblies under mechanical strain and initiate necessary replacements promptly on identifying wear and tear A...

Страница 91: ...e of tubes with disinfectant x Service the head mount on page 80 x Cleaning cones on page 80 X Check replace the O rings on page 81 x Check the function of the louver on page 831 x Perform a precision...

Страница 92: ...as a whole In this case we recommend applying the WHO safety recommendations WHO Labora tory Biosafety Manual For servicing which involves FeliX heads or liquid handling adapters the operator is oblig...

Страница 93: ...ries which were removed for cleaning back into the decks 10 Close the louver and clean the device from the outside 11 Wait until all cleaned surfaces have completely dried 12 Insert the mains plug bac...

Страница 94: ...ipe off any excess grease 8 3 3 Cleaning cones For security reasons and to ensure tightness it is important that the cones of the liquid handling adapters are free of soiling when using these adapters...

Страница 95: ...s If a maintenance contract is in place the Analytik Jena GmbH service department will perform an O ring replacement at each visit Otherwise the O rings should be replaced if there are visible signs o...

Страница 96: ...y a small amount of pressure finally remove the O ring assembly tool and the O ring from the cone 2 If this attempt at O ring removal was unsuccessful there is still an alternative solution for removi...

Страница 97: ...device 2 Operate the louver and check for ease of movement that all movements are interrupted when opening 1 Turn the side of the O ring assembly tool so that the O ring guide pin is fac ing up and pr...

Страница 98: ...84 Rev B 2022 02 Maintenance Care CyBio FeliX...

Страница 99: ...switch or socket bar for this purpose 5 Disconnect power cord from socket bar or line power socket 6 Remove all microplates reservoirs or accessories 7 Clean and disinfect the pipettor and its compon...

Страница 100: ...86 Rev B 2022 02 Shutting Down CyBio FeliX...

Страница 101: ...Order number Head R 96 250 l CyBio RoboTipTray 96 250 l DW OL3810 26 661 CyBio RoboTipTray 96 250 l DW PCR certified pre sterilized OL3810 25 664 CyBio RoboTipTray 96 250 l DW PCR certified pre steri...

Страница 102: ...TipBox 96 250 l PCR certified pre sterilized OL3811 25 637 S CyBio TipBox 96 250 l PCR certified pre sterilized filter OL3811 25 937 F Head R 96 1000 l CHOICE head CyBio TipRack 96 1000 l PCR certifi...

Страница 103: ...0 5 50 l OL3316 11 342 1 channel CHOICE adapter 0 5 50 l OL3316 11 350 16 channel CHOICE adapter 0 5 50 l OL3316 11 380 24 channel CHOICE adapter 0 5 50 l OL3316 11 390 1 channel adapter head R 96 OL...

Страница 104: ...wash basin 96 DW bottom connection OL3317 11 180 Tip wash station 384 DW bottom con nection OL3317 11 190 TIP Detailed information on available accessories can be obtained directly from Analytik Jena...

Страница 105: ...d environmental provisions of law TIP Biological samples must be treated in accordance with locally binding regulations for the handling of infectious material Hazardous substances and related contain...

Страница 106: ...92 Rev B 2022 02 Disposal CyBio FeliX...

Страница 107: ...otection 18 Flashing code 73 Function Tests 46 Fuse Change 72 High Breaking Capacity Fuse 9 18 G Gasoline 78 I Immersion disinfection 22 Incidin Liquid 21 Installation requirements 43 Intended use 4 K...

Страница 108: ...ty markings 13 Safety symbol 13 Scope of delivery 6 Setting tool information 57 Shock indicator 41 Site requirements 43 Spatial requirements 43 Start up 45 Substances 21 T Transport lock Packaging 40...

Страница 109: ...Order number OL5015 505 25 Description and details refer to page 98 Both automation solutions ensure comprehensive personal protection TIP Safety an aspect you must always consider Also consider the...

Страница 110: ...et1 1 Safety workbench 2 CyBio FeliX 3 Door monitoring set Table 29 Components 1 2 3 1 Schematic diagram Fig 48 Description Description Comments 1 Safety workbench Model Safe 2020 Maxisafe 20201 2 CyB...

Страница 111: ...4 Fig 49 Description Description Comments 1 Transponder PSEN cs1 11 2 Switch PSEN cs1 1p2 3 Type plate Specifications Manufacturer product designation trade name identification model serial number ye...

Страница 112: ...8 Rev B 2022 02 CyBio FeliX A 1 2Version II This version is equipped with an active optoelectronic protective device safety light cur tain Fig 50 Safety workbench 1 Safety workbench 2 Light curtain 1...

Страница 113: ...is intended for safety workbenches e g model Safe 2020 Maxisafe 2020 1 2 5 4 3 1 Schematic diagram of version OL3317 23 460 Fig 51 Description Description Comments 1 2 Light curtains 3 Protected area...

Страница 114: ...people The device is intended for integration into laboratory or safety workbenches and auto mated systems The workspace must be limited To minimize the risk of injury from moving parts it must be int...

Страница 115: ...chinery CyBio FeliX 30 5015 500 24 The safety objectives of the Low Voltage Directive are taken into account We commit to transmit in response to a reasoned request by the market surveillance authorit...

Страница 116: ...ESTOP IN connection Table 32 Description An opening switching element between PIN 2 and PIN 4 interrupts the current flow result ing in an E STOP Description Description Comments PIN 1 5 V supply PIN...

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