DORIC Fiberless and Wireless Optogenetically Synchronized Electrophysiology System, Руководство пользователя

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development), the measurement sequence when a high TTL signal (
>
4 V) is detected, and will stop when
a low TTL signal (
<
0.4 V) is detected.
This source can either be Manual , or from one of the four Digital I/O channels. The Trigger Mode can be
either Triggered or Gated .
c) The Pre-processing (Fig. 3.2 ) filters define the high and low-pass frequency cutoff for electrical signal re-
ceived by the headstage.
3. The LED options (Fig. 3.2 ) are all parameters used to control the light source of the cannula connected to the Fi-Wi
headstage .
a) The Channel options (Fig. 3.2 ) are used to control the LED mode and current.
i. The Mode (Fig. 3.2 ) allows the selection of the pulse sequence mode. At time of writing, only Square
mode is available.
ii. The Maximum current (Fig. 3.2 ) defines the current sent to the cannula LED. For proper function of the
cannula, the current should always be greater than 10 mA.
iii. The Baseline (Fig. 3.2 ) leaves a small offset to the current sent to the LED. It is reccomended to use a
small offset, as a complete shut-down of the LED will induce a spike in the electrical acquisition signal.
b) The Sequence options (Fig. 3.2 ) box is where LED pulse sequence parameters are defined.
i. The Starting Delay (Fig. 3.2 ) sets the delay (in hh:mm:ss:zzz format) before the first pulse.
ii. The Frequency/Period (Fig. 3.2 ) sets the frequency (in Hz) or period (in ms) for the pulse sequence.
For example, a signal at 10 Hz (frequency) will output one pulse every 100 ms (period), whereas a pulse
sequence at 0.5 Hz (frequency) will output one pulse every 2000 ms (period).
iii. The Time ON/Duty Cycle (Fig. 3.2 ) sets the time (in ms) or the duty cycle (in %) for each pulse. The Time
ON must be lower than (1/fre0.005 ms, while the Duty cycle must be below 100 %. These
squares will appear red should an impossible Frequency / time ON be selected.
iv. The Smoothing option is used to change the pulse slope in square pulse sequences. The Edit Edges
button opens the Smoothing Edge(s) window (Fig. 3.3 ).
Figure 3.3: Light Source Smoothing Edge(s) Window
A. The Rise Time box is used to define the duration to rise from 0 to the pulse maximum.
B. The Plateau Time box is used to defined the duration the pulse is at its maximum value.
C. The Fall Time box is used to define the duration to descend from the pulse maximum to 0.
D. The Pulse Graph displays the pulse shape.
E. The Active Time box displays the total duration of the pulse. While the Smoothing option is active,
the Time ON is fixed at this value.
(need to include section
v. The Pulses per sequence (Fig. 3.2 ) sets the number of pulses per sequence. If it is set to 0, the pulse will
be repeated indefinitely.
vi. The Number of sequences (Fig. 3.2 ) sets the number of times that the sequence will be repeated. If it is
set to 0, the sequence will be repeated indefinitely.
vii. The Delay between sequences (Fig. 3.2 ) sets the delay (in hh:mm:ss:zzz format) between each sequence
if the Number of Sequences is greater than 1.
viii. The Total Duration (Fig. 3.2 ) displays the total time of the experiment. The different values can be Inf
for infinite, a valid time value or Err if the Time ON value is greater than 1/frequency.
c) The Preview box shows a preview of the pulse sequence.
Chapter 3. Doric Neuroscience Studio 14