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Troubleshooting Guide
Problem
Things to try:
Low DNA
recovery
Run some of the untreated and RapidTip2 purified
PCR on a gel to check your amplicon band
intensity.
Note: Purify at least 45 μl of your PCR reaction to
optimize DNA recovery.
Slow or
difficult
aspiration
Check that pipettor volume is
50 or 60 μl??
.
Check that tips are firmly attached to the pipettor.
Note: Diffinity RapidTip2 is incompatible with
detergents, mineral oil, and ready-to-load PCR mixes
that contain density increasing compounds.
Fluid
remains
in the
RapidTip2
It’s normal for a small amount of liquid (~10 μl) to
remain inside the Diffinity RapidTip2.
Note: Overdispense or blowout all fluid on the last
dispense cycle.
Failure to
remove
impurities
Verify that particles are at the bottom of the
tip prior to sample treatment; the pipettor is
operating at the proper (slow) speed settings;
and that particles are effectively mixing with the
sample during pipetting.
Note: Remove trapped air bubbles by gently “flicking”
the pipettor downward for additional aspirate/dispense
cycles.
Visit www.diffinitygenomics.com/FAQs.asp for additional
information.
