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Guidelines — Pipetting with the RapidTip2
Careful pipetting is important to achieve effective purification
with the RapidTip2. The following pipetting guidelines will help
ensure optimal results:
1. Pre-wet the particles
On the first aspirate step, aspirate approximately the first
half of the sample and pause 5 seconds to ensure complete
wetting of the particles before mixing. Then aspirate the rest
of the sample and dispense.
2. Mix effectively
For effective mixing, it is important that the particles
remain in contact with the sample during each aspirate and
dispense cycle.
Aspirate slowly for the first several cycles until the particles
are completely dispersed into the PCR sample.
Avoid long delays between successive aspirate and dispense
cycles that allow the particles to settle out of solution for
more than 1–2 seconds.
3. Maintain tip-to-sample contact
During aspiration, maintain continuous contact between
the end of the pipette tip and your sample to avoid
aspirating air bubbles in the sample.
