Diffinity RapidTip2
Page 13
3. Place pipette tip into 50 μl PCR solution.
4. Pre-wet the particles on first aspirate (see page 10).
Aspirate about half the sample and then pause for 5 seconds to ensure
complete wetting of the particles before mixing. Aspirate the rest of the
sample and then dispense.
5. Set timer and mix for 60 seconds (approximately 15
aspirate/dispense cycles).
Please note that pipetting will be slower than normal — wait for liquid
to completely fill the tip to begin the next mix. It is not necessary to
drive liquid completely out of the tip on every dispense. The particles
should mix completely with the solution and make it appear cloudy
while inside the tip.
6. Dispense all solution into a clean tube when mixing
is complete. Use your pipettor’s blowout mode for
maximum liquid recovery.
Your purified PCR Amplicon is now ready for your downstream
application.
Quantifying DNA Recovery
If you wish to confirm purification, run unpurified and purified
sample in adjacent lanes on a gel to confirm the amplicon
band. To estimate percent recovery, analyses of the samples
pre and post-purification are necessary. For this process, we
recommend either a PicoGreen
®
2 type assay or visualization on
agarose gel.
