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www.diffinitygenomics.com
Diffinity
™
Technology Principle
The Diffinity RapidTip2 contains everything you need for PCR
purification. The tip is filled with our proprietary adsorption
technology, which has a differential affinity for PCR reaction
components. The impurities are removed from the solution as
it enters the pipette tip. Dispensing the solution yields purified,
high quality DNA ready to use for downstream applications.
You won’t need additional equipment, reagents, buffers, or
enzymes — only a pipettor!
Diffinity RapidTip2 purifies PCR
solutions and removes DNA
polymerase comparably to
popular spin column methods.
The positive control (+) con-
tains polymerase and all PCR
reagents but did not undergo
purification and therefore has
a 600bp band. The control
purification (C) and RapidTip2
purification (R) do not have
bands showing that polymerase
was removed during purifica-
tion.
Diffinity RapidTip2 effectively
removes dNTPs (not visible on
gel), primers (20bp), and primer-
dimers (50bp). The PCR product
was divided and subjected to PCR
purification with either competitor’s
products (C) or RapidTip2 (RT2: 1,
2, 3). RapidTip2 has high yield: the
PCR amplicon (2Kb) shows no loss
of band intensity after purification.
20bp--
50bp--
2Kb--
U C C 1 2 3
---RT2---
+
-
C R
Standard
Hot Start
Proofreading
